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Product Info Summary
| SKU: | M00604 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, ICC, WB |
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Product info
Product Name
Anti-ADAM17/Tace Rabbit Monoclonal Antibody
View all TACE/ADAM17 Antibodies
SKU/Catalog Number
M00604
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ADAM17/Tace Rabbit Monoclonal Antibody catalog # M00604. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ADAM17/Tace Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00604)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AOIO-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ADAM17
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00604 is reactive to ADAM17 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
93 kDa
Calculated molecular weight
93 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00604 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50
FC 1:100
Positive Control
WB: human Jurkat whole cell, rat liver tissue, mouse liver tissue
ICC/IF: Hela cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ADAM17 using anti-ADAM17 antibody (M00604).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: rat liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAM17 antigen affinity purified polyclonal antibody (Catalog # M00604) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAM17 at approximately 93 kDa. The expected band size for ADAM17 is at 93 kDa.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunofluorescent analysis of Hela cells, using ADAM17 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For ADAM17 (Source: Uniprot.org, NCBI)
Gene Name
ADAM17
Full Name
Disintegrin and metalloproteinase domain-containing protein 17
Weight
93 kDa
Alternative Names
Disintegrin and metalloproteinase domain-containing protein 17;ADAM 17;3.4.24.86;Snake venom-like protease;TNF-alpha convertase;TNF-alpha-converting enzyme;CD156b;ADAM17;CSVP, TACE; ADAM17 ADAM18, CD156B, CSVP, NISBD, NISBD1, TACE ADAM metallopeptidase domain 17 disintegrin and metalloproteinase domain-containing protein 17|ADAM metallopeptidase domain 18|TNF-alpha convertase|TNF-alpha convertase enzyme|TNF-alpha converting enzyme|a disintegrin and metalloproteinase 17|cartilage snake venom-like protease|snake venom-like protease|tumor necrosis factor, alpha, converting enzyme
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ADAM17, check out the ADAM17 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ADAM17: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ADAM17/Tace Rabbit Monoclonal Antibody (M00604)
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Customer Q&As
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17 Customer Q&As for Anti-ADAM17/Tace Rabbit Monoclonal Antibody
Question
Is the immunogen sequence for M00604 raised against the N or C terminal?
Verified customer
Asked: 2022-05-16
Answer
The immunogen sequence for Anti-ADAM17/Tace Rabbit Monoclonal Antibody (M00604) was raised against the C terminal aa767-780.
Boster Scientific Support
Answered: 2022-05-17
Question
We have seen staining in human liver. Do you have any suggestions? Is anti-ADAM17/Tace Rabbit Monoclonal antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
Based on literature liver does express ADAM17. Based on Uniprot.org, ADAM17 is expressed in oocyte, leukocyte monocyte, cartilage, cervix carcinoma, erythroleukemia, liver, among other tissues. Regarding which tissues have ADAM17 expression, here are a few articles citing expression in various tissues:
Cartilage, Pubmed ID: 9574564, 12058067
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Erythroleukemia, Pubmed ID: 23186163
Leukocyte, and Monocyte, Pubmed ID: 9034191
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2020-04-27
Question
We are currently using anti-ADAM17/Tace Rabbit Monoclonal antibody M00604 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
The anti-ADAM17/Tace Rabbit Monoclonal antibody (M00604) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-16
Question
Would anti-ADAM17/Tace Rabbit Monoclonal antibody M00604 work for Flow Cytometry with oocyte?
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
According to the expression profile of oocyte, ADAM17 is highly expressed in oocyte. So, it is likely that anti-ADAM17/Tace Rabbit Monoclonal antibody M00604 will work for Flow Cytometry with oocyte.
Boster Scientific Support
Answered: 2020-03-20
Question
We ordered your anti-ADAM17/Tace Rabbit Monoclonal antibody for IP on erythroleukemia a few months ago. I am using rat, and We are going to use the antibody for ICC next. I am interested in examining erythroleukemia as well as liver in our next experiment. Could you please give me some suggestion on which antibody would work the best for ICC?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
I have checked the website and datasheets of our anti-ADAM17/Tace Rabbit Monoclonal antibody and I see that M00604 has been tested on rat in both IP and ICC. Thus M00604 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-01-24
Question
Does M00604 anti-ADAM17/Tace Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
You can see on the product datasheet, M00604 anti-ADAM17/Tace Rabbit Monoclonal antibody as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-11
Question
I see that the anti-ADAM17/Tace Rabbit Monoclonal antibody M00604 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-21
Question
See attached the WB image, lot number and protocol we used for oocyte using anti-ADAM17/Tace Rabbit Monoclonal antibody M00604. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-15
Question
I was wanting to use using your anti-ADAM17/Tace Rabbit Monoclonal antibody for positive regulation of g1/s transition of mitotic cell cycle studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
Thanks for your inquiry. This M00604 anti-ADAM17/Tace Rabbit Monoclonal antibody is tested on hela cells. It is guaranteed to work for Flow Cytometry, IP, IF, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-08-12
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for oocyte using anti-ADAM17/Tace Rabbit Monoclonal antibody M00604. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-13
Question
My boss were well pleased with the WB result of your anti-ADAM17/Tace Rabbit Monoclonal antibody. However we have seen positive staining in leukocyte monocyte membrane using this antibody. Is that expected? Could you tell me where is ADAM17 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-11-16
Answer
From literature, leukocyte monocyte does express ADAM17. Generally ADAM17 expresses in membrane. Regarding which tissues have ADAM17 expression, here are a few articles citing expression in various tissues:
Cartilage, Pubmed ID: 9574564, 12058067
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Erythroleukemia, Pubmed ID: 23186163
Leukocyte, and Monocyte, Pubmed ID: 9034191
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2018-11-16
Question
Is this M00604 anti-ADAM17/Tace Rabbit Monoclonal antibody reactive to the isotypes of ADAM17?
Verified Customer
Verified customer
Asked: 2018-09-27
Answer
The immunogen of M00604 anti-ADAM17/Tace Rabbit Monoclonal antibody is A synthesized peptide derived from human ADAM17. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-09-27
Question
I would like to test anti-ADAM17/Tace Rabbit Monoclonal antibody M00604 on human oocyte for research purposes, then I may be interested in using anti-ADAM17/Tace Rabbit Monoclonal antibody M00604 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-09-14
Answer
The products we sell, including anti-ADAM17/Tace Rabbit Monoclonal antibody M00604, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-09-14
Question
Is a blocking peptide available for product anti-ADAM17/Tace Rabbit Monoclonal antibody (M00604)?
Verified Customer
Verified customer
Asked: 2018-06-19
Answer
We do provide the blocking peptide for product anti-ADAM17/Tace Rabbit Monoclonal antibody (M00604). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-06-19
Question
Is there a BSA free version of anti-ADAM17/Tace Rabbit Monoclonal antibody M00604 available?
L. Roberts
Verified customer
Asked: 2017-08-04
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ADAM17/Tace Rabbit Monoclonal antibody M00604 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-08-04
Question
Can you help my question with product M00604, anti-ADAM17/Tace Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
T. Edwards
Verified customer
Asked: 2017-03-23
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00604 anti-ADAM17/Tace Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-03-23
Question
I was wanting to use your anti-ADAM17/Tace Rabbit Monoclonal antibody for Flow Cytometry for human oocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human oocyte identification?
J. Collins
Verified customer
Asked: 2013-01-21
Answer
You can see on the product datasheet, M00604 anti-ADAM17/Tace Rabbit Monoclonal antibody has been validated for Flow Cytometry, IP, IF, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human oocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-01-21
