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Product Info Summary
| SKU: | A00604 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-ADAM17 Antibody Picoband®
View all TACE/ADAM17 Antibodies
SKU/Catalog Number
A00604
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ADAM17 Antibody Picoband® catalog # A00604. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ADAM17 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00604)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human ADAM17 recombinant protein (Position: R215-Y433).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00604 is reactive to ADAM17 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
78 kDa
Calculated molecular weight
93.021kDa
Background of TACE/ADAM17
ADAM17 (ADAM metallopeptidase domain 17), also called TACE (tumor necrosis factor-α-converting enzyme), is a 70-kDa enzyme that belongs to the ADAM protein family of disintegrins and metalloproteases. Expression studies showed that the encoded protein cleaves precursor tumor necrosis factor-alpha to its mature form. Northern blot analysis revealed that the gene was expressed as a 5-kb mRNA in all tissues examined. ADAM17 is understood to be involved in the processing of tumor necrosis factor alpha (TNF-α) at the surface of the cell, and from within theintracellular membranes of the trans-Golgi network. This process, which is also known as 'shedding', involves the cleavage and release of a soluble ectodomain from membrane-bound pro-proteins (such as pro-TNF-α), and is of known physiological importance. ADAM17 was the first 'sheddase' to be identified, and is also understood to play a role in the release of a diverse variety of membrane-anchored cytokines, cell adhesion molecules,receptors, ligands, and enzymes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00604 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: mouse thymus tissue, human Hela whole cell, human placenta tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ADAM17 using anti-ADAM17 antibody (A00604).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse thymus tissue lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human placenta tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAM17 antigen affinity purified polyclonal antibody (Catalog # A00604) at 0.5 ug/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAM17 at approximately 78KD. The expected band size for ADAM17 is at 97KD.
Protein Target Info & Infographic
Gene/Protein Information For ADAM17 (Source: Uniprot.org, NCBI)
Gene Name
ADAM17
Full Name
Disintegrin and metalloproteinase domain-containing protein 17
Weight
93.021kDa
Alternative Names
Disintegrin and metalloproteinase domain-containing protein 17; ADAM 17; 3.4.24.86; Snake venom-like protease; TNF-alpha convertase; TNF-alpha-converting enzyme; CD156b; ADAM17; CSVP, TACE; ADAM17 ADAM18, CD156B, CSVP, NISBD, NISBD1, TACE ADAM metallopeptidase domain 17 disintegrin and metalloproteinase domain-containing protein 17|ADAM metallopeptidase domain 18|TNF-alpha convertase|TNF-alpha convertase enzyme|TNF-alpha converting enzyme|a disintegrin and metalloproteinase 17|cartilage snake venom-like protease|snake venom-like protease|tumor necrosis factor, alpha, converting enzyme
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ADAM17, check out the ADAM17 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ADAM17: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ADAM17 Antibody Picoband® (A00604)
Hello CJ!
A00604 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Anti-arthritis effect of a novel quinazoline derivative through inhibiting production of TNF-α mediated by TNF-α converting enzyme in murine collagen-induced arthritis model
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-ADAM17 Antibody Picoband®
Question
Can you help my question with product A00604, anti-ADAM17 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00604 anti-ADAM17 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-03
Question
Is this A00604 anti-ADAM17 antibody reactive to the isotypes of ADAM17?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
The immunogen of A00604 anti-ADAM17 antibody is E. coli-derived human ADAM17 recombinant protein (Position: R215-Y433). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-03
Question
I see that the anti-ADAM17 antibody A00604 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-02
Question
Our lab want to know about to test anti-ADAM17 antibody A00604 on human cartilage for research purposes, then I may be interested in using anti-ADAM17 antibody A00604 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Kulkarni
Verified customer
Asked: 2019-11-18
Answer
The products we sell, including anti-ADAM17 antibody A00604, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-18
Question
Is a blocking peptide available for product anti-ADAM17 antibody (A00604)?
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
We do provide the blocking peptide for product anti-ADAM17 antibody (A00604). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-22
Question
I was wanting to use your anti-ADAM17 antibody for ELISA for human cartilage on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cartilage identification?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
As indicated on the product datasheet, A00604 anti-ADAM17 antibody has been tested for ELISA, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cartilage in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-25
Question
We have been able to see staining in rat cartilage. What should we do? Is anti-ADAM17 antibody supposed to stain cartilage positively?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
Based on literature cartilage does express ADAM17. Based on Uniprot.org, ADAM17 is expressed in oocyte, leukocyte monocyte, cartilage, cervix carcinoma, erythroleukemia, liver, among other tissues. Regarding which tissues have ADAM17 expression, here are a few articles citing expression in various tissues:
Cartilage, Pubmed ID: 9574564, 12058067
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Erythroleukemia, Pubmed ID: 23186163
Leukocyte, and Monocyte, Pubmed ID: 9034191
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2019-07-31
Question
Here is the WB image, lot number and protocol we used for cartilage using anti-ADAM17 antibody A00604. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-14
Question
I just want to ask; Is there any cross-reactivity known for this A00604 Anti-ADAM17 Antibody to other species other than human, or is there none to be expected?
Verified Customer
Verified customer
Asked: 2018-06-15
Answer
The A00604 Anti-ADAM17 Antibody cross-reacts with mouse and rat. Other species have not been tested.
Boster Scientific Support
Answered: 2018-06-15
Question
We are currently using anti-ADAM17 antibody A00604 for rat tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-07
Answer
The anti-ADAM17 antibody (A00604) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-07
Question
Is there a BSA free version of anti-ADAM17 antibody A00604 available?
Verified Customer
Verified customer
Asked: 2018-01-12
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ADAM17 antibody A00604 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-01-12
Question
We purchased anti-ADAM17 antibody for WB on oocyte in a previous project. I am using rat, and We intend to use the antibody for ELISA next. I am interested in examining oocyte as well as cartilage in our next experiment. Do you have any suggestion on which antibody would work the best for ELISA?
H. Krishna
Verified customer
Asked: 2017-11-17
Answer
I viewed the website and datasheets of our anti-ADAM17 antibody and I see that A00604 has been validated on rat in both WB and ELISA. Thus A00604 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-11-17
Question
Would A00604 anti-ADAM17 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-07-27
Answer
It shows on the product datasheet, A00604 anti-ADAM17 antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-07-27
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cartilage using anti-ADAM17 antibody A00604. Let me know if you need anything else.
D. Jackson
Verified customer
Asked: 2016-12-01
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-12-01
Question
Our team were satisfied with the WB result of your anti-ADAM17 antibody. However we have been able to see positive staining in cervix carcinoma membrane using this antibody. Is that expected? Could you tell me where is ADAM17 supposed to be expressed?
A. Krishna
Verified customer
Asked: 2016-08-08
Answer
Based on literature, cervix carcinoma does express ADAM17. Generally ADAM17 expresses in membrane. Regarding which tissues have ADAM17 expression, here are a few articles citing expression in various tissues:
Cartilage, Pubmed ID: 9574564, 12058067
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Erythroleukemia, Pubmed ID: 23186163
Leukocyte, and Monocyte, Pubmed ID: 9034191
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2016-08-08
Question
Would anti-ADAM17 antibody A00604 work for ELISA with cartilage?
M. Walker
Verified customer
Asked: 2016-02-02
Answer
According to the expression profile of cartilage, ADAM17 is highly expressed in cartilage. So, it is likely that anti-ADAM17 antibody A00604 will work for ELISA with cartilage.
Boster Scientific Support
Answered: 2016-02-02
Question
Hello Boster; your datasheet in A00604 Anti-ADAM17 Antibody states that this antibody cross-reacts with mouse tissue. Could you please tell me in which mouse cell types this antibody was tested in Western blot applications? Thank you
Verified Customer
Verified customer
Asked: 2014-11-03
Answer
The A00604 Anti-ADAM17 Antibody has been tested in mouse lung, brain, liver, kidney, spleen, and 3T3 cells.
Boster Scientific Support
Answered: 2014-11-03
