Click image to see more details
Product Info Summary
| SKU: | A04879-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Wnt2b Antibody Picoband®
SKU/Catalog Number
A04879-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Wnt2b Antibody Picoband® catalog # A04879-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Wnt2b Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04879-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Wnt2b recombinant protein (Position: A7-T391).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04879-1 is reactive to WNT2B in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
60 kDa
Calculated molecular weight
35883 MW
Background of Wnt-2b
WNT2B (Wingless-Type MMTV Integration Site Family Member 2B), is a protein that in humans is encoded by the WNT2B gene. This gene encodes a member of the wingless-type MMTV integration site (WNT) family of highly conserved, secreted signaling factors. Katoh et al. (1996) used fluorescence in situ hybridization to map the WNT13 gene to chromosome 1p13. WNT family members function in a variety of developmental processes including regulation of cell growth and differentiation and are characterized by a WNT-core domain. This gene may play a role in human development as well as human carcinogenesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04879-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human HEK293 whole cell, human HEPG2 whole cell, human Jurkat whole cell, human A549 whole cell, human Raji whole cell, human SW620 whole cell, human SKOV3 whole cell, rat PC-12 whole cell, mouse Neuro-2a whole cell, mouse ANA-1 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Wnt2b using anti-Wnt2b antibody (A04879-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates,
Lane 2: human HEPG2 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: human Raji whole cell lysates,
Lane 6: human SW620 whole cell lysates,
Lane 7: human SKOV3 whole cell lysates,
Lane 8: rat PC-12 whole cell lysates,
Lane 9: mouse Neuro-2a whole cell lysates,
Lane 10: mouse ANA-1 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Wnt2b antigen affinity purified polyclonal antibody (Catalog # A04879-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Wnt2b at approximately 60KD. The expected band size for Wnt2b is at 60KD.
Protein Target Info & Infographic
Gene/Protein Information For WNT2B (Source: Uniprot.org, NCBI)
Gene Name
WNT2B
Full Name
Protein Wnt-2b
Weight
35883 MW
Superfamily
Wnt family
Alternative Names
Protein O-mannosyl-transferase 2; Dolichyl-phosphate-mannose--protein mannosyltransferase 2; POMT2 WNT2B WNT13 Wnt family member 2B protein Wnt-2b|XWNT2, Xenopus, homolog of|wingless-type MMTV integration site family, member 13|wingless-type MMTV integration site family, member 2B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on WNT2B, check out the WNT2B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for WNT2B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Wnt2b Antibody Picoband® (A04879-1)
Hello CJ!
A04879-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xu T,Pan L,Li L,Hu S,Zhou H,Yang C,Yang J,Li H,Liu Y,Meng X,Li J.MicroRNA-708 modulates Hepatic Stellate Cells activation and enhances extracellular matrix accumulation via direct targeting TMEM88. J Cell Mol Med. 2020 Jul;24(13):7127-7140.doi: 10.1111/jc
Species: Human
A04879-1 usage in article: APP:WB, SAMPLE:LX-2 CELLS, DILUTIO N:NA
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Wnt2b Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Wnt2b Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
