Anti-G-protein coupled receptor 30/GPER1 Antibody Picoband®

Product Name

Anti-G-protein coupled receptor 30/GPER1 Antibody Picoband®

View all GPER/GPR30 Antibodies

SKU/Catalog Number

PA2235

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-G-protein coupled receptor 30/GPER1 Antibody catalog # PA2235. Tested in WB applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-G-protein coupled receptor 30/GPER1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2235)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human G-protein coupled receptor 30, different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2235 is reactive to GPER1 in Human, Monkey

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

60 kDa

Calculated molecular weight

42248 MW

Background of GPER/GPR30

G-protein coupled estrogen receptor 1 (GPER1), also known as the GPR30 or CEPR is a G protein-coupled receptor that in humans is encode d by the GPER gene. This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum. It is mapped to 7p22.3. The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. Alternate transcriptional splice variants which encode the same protein have been characterized.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA2235 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Monkey

Positive Control

WB: COLO320 Cell, MCF-7 Cell, COS7 Cell

Validation Images & Assay Conditions

Gene/Protein Information For GPER1 (Source: Uniprot.org, NCBI)

Alternative Names

G-protein coupled estrogen receptor 1;Chemoattractant receptor-like 2;Flow-induced endothelial G-protein coupled receptor 1;FEG-1;G protein-coupled estrogen receptor 1;G-protein coupled receptor 30;GPCR-Br;IL8-related receptor DRY12;Lymphocyte-derived G-protein coupled receptor;LYGPR;Membrane estrogen receptor;mER;GPER1;CEPR, CMKRL2, DRY12, GPER, GPR30; GPER1 CEPR, CMKRL2, DRY12, FEG-1, GPCR-Br, GPER, GPR30, LERGU, LERGU2, LyGPR, mER G protein-coupled estrogen receptor 1 G-protein coupled estrogen receptor 1|G protein-coupled receptor 30|IL8-related receptor DRY12|chemoattractant receptor-like 2|chemokine receptor-like 2|constitutively expressed peptide-like receptor|flow-induced endothelial G-protein coupled receptor 1|heptahelix receptor|lymphocyte-derived G-protein coupled receptor|membrane estrogen receptor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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