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Product Info Summary
| SKU: | A01975 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Actinin Alpha 4 ACTN4 Antibody
View all Alpha Actinin 4 Antibodies
SKU/Catalog Number
A01975
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Actinin Alpha 4 ACTN4 Antibody catalog # A01975. Tested in WB,ICC,IF,IP,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Actinin Alpha 4 ACTN4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01975)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01975 is reactive to ACTN4 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
104854 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01975 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2,000
ICC: 1:100-1:500
IHC: 1:50-1:200
FC: 1:50-1:100
IP: 1:10-1:50
Validation Images & Assay Conditions
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Figure 2. Immunocytochemistry staining of ACTN4 using Anti-Actinin Alpha 4 ACTN4 Antibody (A01975).
ICC staining alpha Actinin 4 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde
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Figure 1. Western blotting validation for Anti-Actinin Alpha 4 ACTN4 Antibody A01975
Western blot analysis of alpha Actinin 4 on different lysates using anti-alpha Actinin 4 antibody at 1/500 dilution.Positive control:
Lane 1: Hela
Lane 2: PC-12
Lane 3: NIH-3T3
Lane 4: Rat liver tissue
Lane 5: A431
Lane 6: HepG2
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For ACTN4 (Source: Uniprot.org, NCBI)
Gene Name
ACTN4
Full Name
Alpha-actinin-4
Weight
104854 MW
Superfamily
alpha-actinin family
Alternative Names
Alpha-actinin-4 ;Non-muscle alpha-actinin 4 ACTN4 ACTININ-4, FSGS, FSGS1 actinin alpha 4 alpha-actinin-4|focal segmental glomerulosclerosis 1|non-muscle alpha-actinin 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ACTN4, check out the ACTN4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ACTN4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Actinin Alpha 4 ACTN4 Antibody (A01975)
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