Product Info Summary
SKU: | PB9124 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, IHC-F, ICC, WB |
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Product info
Product Name
Anti-Angiotensin Converting Enzyme 1/ACE Antibody Picoband®
SKU/Catalog Number
PB9124
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Angiotensin Converting Enzyme 1/ACE Antibody Picoband® catalog # PB9124. Tested in IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Angiotensin Converting Enzyme 1/ACE Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9124)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ACE recombinant protein (Position: K651-Y864). Human ACE shares 73% and 76% amino acid (aa) sequences identity with mouse and rat ACE, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9124 is reactive to ACE in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
180 kDa
Calculated molecular weight
149715 MW
Background of ACE/CD143
Angiotensin-converting enzyme (ACE), an exopeptidase, is a circulating enzyme that participates in the body's renin-angiotensin system (RAS), which mediates extracellular volume (i.e. that of the blood plasma, lymph and interstitial fluid), and arterial vasoconstriction. It is secreted by pulmonary and renal endothelial cells and catalyzes the conversion of decapeptide angiotensin I to octapeptide angiotensin II. Using a DNA marker at the growth hormone gene locus, which they characterized as 'extremely polymorphic' and which showed no recombination with ACE, ACE was mapped to 17q22-q24, consistent with the in situ hybridization mapping to 17q23. ACE, or kininase II, is a dipeptidyl carboxypeptidase that plays an important role in blood pressure regulation and electrolyte balance by hydrolyzing angiotensin I into angiotensin II, a potent vasopressor, and aldosterone-stimulating peptide. The enzyme is also able to inactivate bradykinin, a potent vasodilator.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9124 is guaranteed for IF, IHC, IHC-F, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Positive Control
WB: rat lung tissue, mouse lung tissue, human Raji whole cell
IHC: human lung cancer tissue, human thyroid cancer tissue, mouse lung tissue, rat lung tissue
IHC-F: human placenta tissue, mouse lung tissue
ICC/IF: A431 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ACE using anti-ACE antibody (PB9124).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: mouse lung tissue lysates,
Lane 3: human Raji whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACE antigen affinity purified polyclonal antibody (Catalog # PB9124) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACE at approximately 180KD. The expected band size for ACE is at 150KD.
Click image to see more details
Figure 2. IHC analysis of ACE using anti-ACE antibody (PB9124).
ACE was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ACE Antibody (PB9124) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of ACE using anti-ACE antibody (PB9124).
ACE was detected in paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ACE Antibody (PB9124) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of ACE using anti-ACE antibody (PB9124).
ACE was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ACE Antibody (PB9124) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of ACE using anti-ACE antibody (PB9124).
ACE was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ACE Antibody (PB9124) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 6. IHC analysis of ACE using anti-ACE antibody (PB9124).
ACE was detected in frozen section of human placenta tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ACE Antibody (PB9124) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 7. IHC analysis of ACE using anti-ACE antibody (PB9124).
ACE was detected in frozen section of mouse lung tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ACE Antibody (PB9124) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 8. IF analysis of ACE using anti-ACE antibody (PB9124).
ACE was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ACE Antibody (PB9124) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For ACE (Source: Uniprot.org, NCBI)
Gene Name
ACE
Full Name
Angiotensin-converting enzyme
Weight
149715 MW
Superfamily
peptidase M2 family
Alternative Names
Angiotensin-converting enzyme;ACE;3.2.1.-;3.4.15.1;Dipeptidyl carboxypeptidase I;Kininase II;CD143;Angiotensin-converting enzyme, soluble form;ACE;DCP, DCP1; ACE ACE1, CD143, DCP, DCP1 angiotensin I converting enzyme angiotensin-converting enzyme|CD143 |angiotensin I converting enzyme (peptidyl-dipeptidase A) 1|carboxycathepsin|dipeptidyl carboxypeptidase 1|dipeptidyl carboxypeptidase I|kininase II|peptidase P
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ACE, check out the ACE Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ACE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Angiotensin Converting Enzyme 1/ACE Antibody Picoband® (PB9124)
Hello CJ!
PB9124 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Low concentrations of bilirubin inhibit activation of hepatic stellate cells in vitro
Local renin-angiotensin system is associated with bone mineral density of glucocorticoid-induced osteoporosis patients
Telmisartan protects chronic intermittent hypoxic mice via modulating cardiac renin-angiotensin system activity
Aliskiren has chondroprotective efficacy in a rat model of osteoarthritis through suppression of the local renin-angiotensin system
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Customer Q&As
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5 Customer Q&As for Anti-Angiotensin Converting Enzyme 1/ACE Antibody Picoband®
Question
We are currently using anti-Angiotensin Converting Enzyme 1/ACE antibody PB9124 for rat tissue, and we are well pleased with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
The anti-Angiotensin Converting Enzyme 1/ACE antibody (PB9124) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-14
Question
Our lab used your anti-Angiotensin Converting Enzyme 1/ACE antibody for IHC-P on testis in the past. I am using human, and We want to use the antibody for IF next. you antibody examining testis as well as umbilical vein endothelial cell in our next experiment. Do you have any suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
I have checked the website and datasheets of our anti-Angiotensin Converting Enzyme 1/ACE antibody and it seems that PB9124 has been tested on human in both IHC-P and IF. Thus PB9124 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-07-18
Question
Our lab were well pleased with the WB result of your anti-Angiotensin Converting Enzyme 1/ACE antibody. However we have observed positive staining in lung angiotensin-converting enzyme using this antibody. Is that expected? Could you tell me where is ACE supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
Based on literature, lung does express ACE. Generally ACE expresses in angiotensin-converting enzyme, soluble form:, cell membrane. Regarding which tissues have ACE expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 16625196
Liver, Pubmed ID: 19159218
Lung, Pubmed ID: 2558109
Plasma, Pubmed ID: 16335952
Testis, Pubmed ID: 14702039
Umbilical vein endothelial cell, Pubmed ID: 9642152
Boster Scientific Support
Answered: 2019-07-04
Question
I am interested in using your anti-Angiotensin Converting Enzyme 1/ACE antibody for positive regulation of blood pressure studies. Has this antibody been tested with western blotting on mouse lung tissue? We would like to see some validation images before ordering.
C. Moore
Verified customer
Asked: 2016-08-17
Answer
Thanks for your inquiry. This PB9124 anti-Angiotensin Converting Enzyme 1/ACE antibody is validated on rat lung tissue, mouse lung tissue, human placenta tissue. It is guaranteed to work for IF, IHC-P, IHC-F, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-08-17
Question
We have seen staining in mouse testis. Are there any suggestions? Is anti-Angiotensin Converting Enzyme 1/ACE antibody supposed to stain testis positively?
E. Parker
Verified customer
Asked: 2015-01-13
Answer
From literature testis does express ACE. From Uniprot.org, ACE is expressed in testis, brain, lung, umbilical vein endothelial cell, plasma, liver, among other tissues. Regarding which tissues have ACE expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 16625196
Liver, Pubmed ID: 19159218
Lung, Pubmed ID: 2558109
Plasma, Pubmed ID: 16335952
Testis, Pubmed ID: 14702039
Umbilical vein endothelial cell, Pubmed ID: 9642152
Boster Scientific Support
Answered: 2015-01-13