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Product Info Summary
| SKU: | MA1008 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Bovine, Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Calcineurin Alpha Ppp3r1 Antibody (Monoclonal, CN-A1)
View all Calcineurin B Antibodies
SKU/Catalog Number
MA1008
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Calcineurin Alpha Ppp3r1 Antibody (Monoclonal, CN-A1) catalog # MA1008. Tested in IHC, WB applications. This antibody reacts with Bovine, Human, Mouse, Rat.
Storage & Handling
At -20˚C for one year and at 4˚C for one month from date of receipt. Avoid repeated freezing and thawing.
Cite This Product
Anti-Calcineurin Alpha Ppp3r1 Antibody (Monoclonal, CN-A1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1008)
Host
Mouse
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clonality
Monoclonal
Clone Number
CN-A1
Isotype
Mouse IgG1
Immunogen
Bovine brain calcineurin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1008 is reactive to Ppp3r1 in Bovine, Human, Mouse, Rat
Reconstitution
Adding 1 ml of PBS buffer will yield a concentration of 100 ug/ml.
Observed Molecular Weight
61 kDa
Calculated molecular weight
19300 MW
Background of Calcineurin B
Calcineurin, the Ca (2+)/calmodulin-regulated protein phosphatase, first detected in skeletal muscle and brain, has been found in all cells from yeast to mammals. Calcineurin A alpha (PPP3CA),is located on human chromosomes 4, Chromosomal mapping of the human genes for the calmodulin-dependent protein phosphatase (calcineurin) catalytic subunit. Calcineurin regulates bone formation by the osteoblast.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1008 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, bovine, mouse, rat, By Heat
Western blot, 0.25-0.5μg/ml, Human, bovine, mouse, rat
Positive Control
WB: rat brain tissue, mouse brain tissue
IHC: mouse brain tissue, rat brain tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Calcineurin Alpha/PPP3R1 using anti-Calcineurin Alpha/PPP3R1 antibody (MA1008).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Calcineurin Alpha/PPP3R1 antigen affinity purified monoclonal antibody (Catalog # MA1008) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Calcineurin Alpha/PPP3R1 at approximately 61 kDa. The expected band size for Calcineurin Alpha/PPP3R1 is at 19 kDa.
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Figure 2. IHC analysis of Calcineurin Alpha/PPP3R1 using anti-Calcineurin Alpha/PPP3R1 antibody (MA1008).
Calcineurin Alpha/PPP3R1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml mouse anti-Calcineurin Alpha/PPP3R1 Antibody (MA1008) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
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Figure 3. IHC analysis of Calcineurin Alpha/PPP3R1 using anti-Calcineurin Alpha/PPP3R1 antibody (MA1008).
Calcineurin Alpha/PPP3R1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml mouse anti-Calcineurin Alpha/PPP3R1 Antibody (MA1008) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Ppp3r1 (Source: Uniprot.org, NCBI)
Gene Name
Ppp3r1
Full Name
Calcineurin subunit B type 1
Weight
19300 MW
Superfamily
calcineurin regulatory subunit family
Alternative Names
Calcineurin subunit B type 1;Protein phosphatase 2B regulatory subunit 1;Protein phosphatase 3 regulatory subunit B alpha isoform 1;Ppp3r1;Cna2, Cnb; PPP3R1 CALNB1, CNB, CNB1 protein phosphatase 3 regulatory subunit B, alpha calcineurin subunit B type 1|calcineurin B, type I (19kDa)|protein phosphatase 2B regulatory subunit 1|protein phosphatase 2B regulatory subunit B alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ppp3r1, check out the Ppp3r1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ppp3r1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Calcineurin Alpha Ppp3r1 Antibody (Monoclonal, CN-A1) (MA1008)
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Customer Q&As
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6 Customer Q&As for Anti-Calcineurin Alpha Ppp3r1 Antibody (Monoclonal, CN-A1)
Question
My question regards to test anti-Calcineurin Alpha antibody (Monoclonal, CN-A1) MA1008 on bovine blood for research purposes, then I may be interested in using anti-Calcineurin Alpha antibody (Monoclonal, CN-A1) MA1008 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
The products we sell, including anti-Calcineurin Alpha antibody (Monoclonal, CN-A1) MA1008, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-29
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-Calcineurin Alpha antibody (Monoclonal, CN-A1) MA1008. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-24
Question
Here is the WB image, lot number and protocol we used for blood using anti-Calcineurin Alpha antibody (Monoclonal, CN-A1) MA1008. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-17
Question
We are currently using anti-Calcineurin Alpha antibody (Monoclonal, CN-A1) MA1008 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says bovine, human, rat. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2017-11-03
Answer
The anti-Calcineurin Alpha antibody (Monoclonal, CN-A1) (MA1008) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-03
Question
Is a blocking peptide available for product anti-Calcineurin Alpha antibody (Monoclonal, CN-A1) (MA1008)?
G. Carter
Verified customer
Asked: 2017-07-05
Answer
We do provide the blocking peptide for product anti-Calcineurin Alpha antibody (Monoclonal, CN-A1) (MA1008). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-07-05
Question
I see that the anti-Calcineurin Alpha antibody (Monoclonal, CN-A1) MA1008 works with IHC, what is the protocol used to produce the result images on the product page?
D. Jones
Verified customer
Asked: 2016-05-06
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-05-06
