Product Info Summary
SKU: | A02979 |
---|---|
Size: | 100ug |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-ABCB5 Antibody
SKU/Catalog Number
A02979
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-ABCB5 Antibody (Catalog # A02979). Tested in ELISA, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store Anti-ABCB5 at -20°C prior to opening. Aliquot antibody and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-ABCB5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02979)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
ABCB5 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues corresponding to an internal region of human ABCB5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02979 is reactive to ABCB5 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Observed Molecular Weight
42 kDa
Calculated molecular weight
42170 MW
Background of Abcb5
This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Anti-ABCB5 Antibody is a ATP-Binding Cassette protein. ABCB5 is a novel member of the human P-glycoprotein family. It functions as a determinant of membrane potential and regulator of cell fusion in physiologic skin progenitor cells. Cell fusion is thought to contribute to tissue differentiation. ABCB5 is predominantly expressed in melanoma cells and is a novel molecular marker for a distinct subset of chemoresistant stem cell phenotype-expressing tumor cells among human epidermal melanocytes. ABCB5 is a rhodamine efflux transporter. This antibody product is intended to be used to confirm cellular localization and expression level of ABCB5. ABCB5 antibody is ideal for Cancer and Signal Transduction research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02979 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:10,000 - 1:50,000
IHC: 1:200
WB: 1:20,000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. IHC analysis of ABCB5 using anti-ABCB5 antibody (A02979).
ABCB5 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ABCB5 Antibody (A02979) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 2. Western blot analysis of ABCB5 using anti-ABCB5 antibody (A02979).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCB5 antigen affinity purified polyclonal antibody (Catalog # A02979) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for ABCB5.
Click image to see more details
Figure 3. IHC analysis of ABCB5 using anti-ABCB5 antibody (A02979).
ABCB5 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ABCB5 Antibody (A02979) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of ABCB5 using anti-ABCB5 antibody (A02979).
ABCB5 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ABCB5 Antibody (A02979) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For ABCB5 (Source: Uniprot.org, NCBI)
Gene Name
ABCB5
Full Name
ATP-binding cassette sub-family B member 5
Weight
42170 MW
Superfamily
ABC transporter superfamily
Alternative Names
ABCB5, ABCB 5, ABCB-5, ATP binding cassette sub family B (MDR/TAP) member 5, ATP binding cassette sub family B member 5, EST422562, P glycoprotein ABCB5 Abcb5|9230106F14Rik|ATP-binding cassette, sub-family B (MDR/TAP), member 5|ATP-binding cassette sub-family B member 5|ABCB5 P-gp|P-glycoprotein ABCB5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ABCB5, check out the ABCB5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ABCB5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ABCB5 Antibody (A02979)
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Customer Q&As
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4 Customer Q&As for Anti-ABCB5 Antibody
Question
Do you have a BSA free version of anti-ABCB5 antibody A02979 available?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ABCB5 antibody A02979 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-25
Question
Is a blocking peptide available for product anti-ABCB5 antibody (A02979)?
M. Wu
Verified customer
Asked: 2018-09-27
Answer
We do provide the blocking peptide for product anti-ABCB5 antibody (A02979). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-09-27
Question
Please see the WB image, lot number and protocol we used for melanoma using anti-ABCB5 antibody A02979. Please let me know if you require anything else.
M. Evans
Verified customer
Asked: 2017-02-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-02-08
Question
Is this A02979 anti-ABCB5 antibody reactive to the isotypes of ABCB5?
R. Johnson
Verified customer
Asked: 2013-01-21
Answer
The immunogen of A02979 anti-ABCB5 antibody is ABCB5 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues corresponding to an internal region of human ABCB5. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-01-21