Abcb5 Antibodies

ATP-binding cassette sub-family B member 5 (Abcb5)

Drug efflux transporter present in several of stem cells that serves as a regulator of cellular differentiation. Willing to mediate efflux from cells of the rhodamine dye as well as the therapeutic drug doxorubicin.

Particularly within limbal stem cells, where it plays an integral role in corneal development and repair. .

Gene Information

Mouse
Gene Name:	Abcb5
Uniprot:	B5X0E4
Entrez:	77706

Family

Belongs to:
ABC transporter superfamily

Alternative Names

ABCB5 P-gp; ABCB5; ABCB5alpha; ABCB5beta; ATP-binding cassette protein; ATP-binding cassette sub-family B member 5; ATP-binding cassette, sub-family B (MDR/TAP), member 5; EC 3.6.3; EC 3.6.3.44; EST422562; P-glycoprotein ABCB5

Molecular Weight

Mass (kDA):
137.397 kDA

Genomic Context

Mouse
Location:	12|12 F2
Sequence:	12;

Tissue Specificity

In developing eye, expressed in basal limbal epithelium but not in central cornea. Acts as a marker of limbal stem cells.

Diseases

• Melanoma
• Neoplasms
• Malignant Neoplasms
• Skin Neoplasms
• Malignant Paraganglionic Neoplasm
• Neoplasm Metastasis
• Cell Transformation, Neoplastic
• Carcinoma
• Tumor Progression
• Malignant Neoplasm Of Breast
• Metastatic Melanoma

• Leukemia
• Metastatic Malignant Neoplasm To The Lung
• Lymphoma
• Mammary Neoplasms
• Adenocarcinoma
• Liver Carcinoma

Pathways

• Drug Resistance
• Transport
• Dna Repair
• Pathogenesis
• Rna Interference
• Cell Dedifferentiation
• Cell Proliferation
• Cell Killing
• Sterol Transport

• Doxorubicin Transport
• Dedifferentiation
• Chromatin Modification
• Secretion

PTMs

• Hydroxylation

• Cleavage

Research Areas

• ABC Transporters
• Lipid and Metabolism

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/Abcb5

Best Uses Of The ABCB5 Marker

Boster Bio has a high-affinity antibody against the ABCB5 gene. ABCB5 is a key drug efflux transporter and gene found in limbal stem cell cells. Boster Bio will provide you with it, regardless of whether you're trying to understand the function of this gene in a disease model or studying its expression.

Boster offers high-affinity primary antibodies to ABCB5

If you're looking for a reliable antibody to analyze the activity of ABCB5, Boster Bio has the right solution for you. The company's primary antibodies have been well validated and cited by the research community for over 25 years. These antibodies have been tested on Western Blotting, Immunohistochemistry, and ELISA, making them a great choice for your research.

ELISA tests are used throughout the development process to confirm antibody quality. Each test-bleed is tested after immunization. For hybridoma-fusion, spleen cells from the best-responding species are taken. After the ELISA assay is complete, positive supernatants must be screened through limiting-dilution cloning until all wells have grown at the same speed. Additional testing is performed on selected antibodies.

ABCB5 refers to a limbal stem cell gene

Scientists have discovered ABCB5 as a gene that codes for limbal stem cells. These cells play a critical role in corneal development and regeneration. Researchers found that LSCs expressing human and murine ABCB5 were capable of successfully restoring the corneas in mice lacking LSCs. They discovered that abcb5 expression is preferentially found in LSCs that retain labels. However, in both human and murine patients, the presence of ABCB5 is reduced. This gene is responsible for corneal differentiation and wound healing defects.

The stromal layer is a layer of specialized squamous epithelia that covers the human corneal rims. For the cornea to remain clear, these cells need to be healthy. This layer must be able to regenerate itself. Additionally to regenerating the cornea limbal stem cells may also be used to treat conjunctivitis. Conjunctivitis is a condition that affects sight.

The researchers isolated corneal epithelial cells from donor corneas and cultured them in DMEM containing 10% FBS. Accutase was used to culture corneas from recipient mice that had deficient limbal stem cells. Donor grafts were also used. The transplanted limbal cells of mice with the defect did not form stratified epithelium.

LSCs were used to extract RNA and then transduce it in vitro. They were then enzymatically separated and seeded on feeder cell with fresh LSC medium. Using a high quality cDNA reverse transcription kit (Applied Biosystems), total RNA from the cells was extracted and transcribed to cDNA. TaqMan Gene Expression Anasays detected p63a CK12 and B2M. This was done in triplets.

The ABCB5 gene is found in the limbus and corneas of both mouse and human eyes. It is necessary for the maintenance, growth, and repair corneal tissues. In addition, knockout mouse models lack the mature epithelium. These cells should not be mistakenly interpreted as a result corneal disease. The limbus will not develop properly if the ABCB5 gene does not function.

Researchers have discovered that the ABCB5 gene is capable of identifying mammalian limbal stem cell. The gene can also be expressed in human epidermal pigment cells. A mouse LSC carrying the ABCB5 genes was identified as a progenitor. ABCB5 is believed to prevent mononuclear cell formation when the gene is blocked. Patients with ABCB5 positive cells are more likely than those with other types to exhibit this phenotype.

ABCB5 can be used to transport drugs.

The multifunctional ABCB5 transducer is expressed in placental embryos and stem cells. It is believed to play a role both in tumorigenesis as well as in trophoblast function. Its biologic relevance will require further research. It is not known what role ABCB5 plays in cancer cell growth and maintenance. Here is a summary of ABCB5's roles in cancer. We also discuss the possible roles of ABCB5 for drug efflux.

ABCB5 is a member of the ATP-binding cassette superfamily. It is found in many human cancers. It has been implicated with chemotherapy failure. Cancer cells that express high levels of ABCB5 also have enhanced metastasis and epithelial-mesenchymal transition. Metastasis is inhibited by knockdown of ABCB5. Further, ZEB1 may be a downstream factor of ABCB5.

The ABCB5 transporter regulates the resistance to doxorubicin in human malignant melanomas. It has also been implicated for colorectal tumors. 5-FU resistance is decreased when the ABCB5 genes are silenced. Further, ABCB5 is implicated in several human cancers, including melanoma, and has other roles in tumorigenesis. Interestingly, ABCB5 regulates melanoma initiating cells' secretion of IL1b. In addition, it promotes epithelial-mesenchymal transition, which inhibits tumor growth in vivo.

ABCC5 regulates ABCB5 and the ability of pemetrexed, in addition to controlling ABCB5 gene expression. ABCC5 overexpression in mice with tumour volumes greater than 150 mm3 resulted in decreased pemetrexed tolerance. The tumor volume was 2.7 times greater than the initial volume. ABCC5 knockdown reduced the survival rate of patients receiving 5-FU treatment.

ABCB5 plays a critical role in melanoma metastasis. It also regulates progenitor cells fusion. In human melanoma, dysfunction of ABCB5 confers chemoresistance. Mutant cells without ABCB5 are more susceptible to doxorubicin. It has been further shown that ABCB5 regulates how human stem cells function. These cells are capable of self regeneration, tumorigenic, and differentiation.

Multidrug resistance is a major obstacle to chemotherapeutic treatment for acute leukemia. Numerous studies have demonstrated the role of ABCB5 as a factor in chemotherapeutic failure. In addition to promoting chemotherapeutic drug efflux, this transporter also inhibits protein synthesis. It can also promote drug metabolic by increasing blood clots' permeability.

In a recent study that used octocrylene as a sunscreen chemical, we investigated the role of ABCB5 and melanoma. OCT has been shown to promote melanoma (the most deadly form of skin cancer). The researchers tested the effects of OCT on melanoma cells using comprehensive metabolomic analysis by phosphorus and proton NMR spectroscopy.

The ABCB5 transportationer is widely found in bacteria, and other microorganisms. The Escherichia Coli genome predicted the existence of 69 ABC transporters. Of these, 11 are drug exporters. The putative drug export transporter YbhFSR has received minimal research. The gene is predicted that it encodes the two ABC transporter components. In addition to YbhF, ybhFSR also encodes a putative drug resistance exporter.