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Product Info Summary
| SKU: | A00895-Dyl488 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human SUR1 DyLight® 488 conjugated ABCC8 Antibody
SKU/Catalog Number
A00895-Dyl488
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human SUR1 DyLight® 488 conjugated ABCC8 Antibody catalog # A00895-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human SUR1 DyLight® 488 conjugated ABCC8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00895-Dyl488)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human SUR1, which shares 97.7% amino acid (aa) sequence identity with both mouse and rat SUR1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00895-Dyl488 is reactive to ABCC8 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
176.992kDa
Background of SUR1
ATP-binding cassette transporter sub-family C member 8 is a protein that in humans is encoded by the ABCC8 gene. The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a modulator of ATP-sensitive potassium channels and insulin release. Mutations and deficiencies in this protein have been observed in patients with hyperinsulinemic hypoglycemia of infancy, an autosomal recessive disorder of unregulated and high insulin secretion. Mutations have also been associated with non-insulin-dependent diabetes mellitus type II, an autosomal dominant disease of defective insulin secretion. Alternatively spliced transcript variants have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00895-Dyl488 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Positive Control
FCM: A431 cell
Validation Images & Assay Conditions
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Figure 1. Flow Cytometry analysis of A431 cells using anti-Human SUR1 antibody (A00895-Dyl488).
Overlay histogram showing A431 cells stained with A00895-Dyl488 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Human SUR1 Antibody (A00895-Dyl488,1μg/1x106 cells) for 30 min at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For ABCC8 (Source: Uniprot.org, NCBI)
Gene Name
ABCC8
Full Name
ATP-binding cassette sub-family C member 8
Weight
176.992kDa
Superfamily
ABC transporter superfamily
Alternative Names
ATP-binding cassette sub-family C member 8; Sulfonylurea receptor 1; ABCC8; HRINS; SUR; SUR1 ABCC8 ABC36, HHF1, HI, HRINS, MRP8, PHHI, PNDM3, SUR, SUR1, SUR1delta2, TNDM2 ATP binding cassette subfamily C member 8 ATP-binding cassette sub-family C member 8|ATP-binding cassette transporter sub-family C member 8|ATP-binding cassette, sub-family C (CFTR/MRP), member 8|sulfonylurea receptor (hyperinsulinemia)|sulfonylurea receptor 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ABCC8, check out the ABCC8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ABCC8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human SUR1 DyLight® 488 conjugated ABCC8 Antibody (A00895-Dyl488)
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