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Product Info Summary
| SKU: | PA1647 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-5HT1A Receptor/HTR1A Antibody Picoband®
SKU/Catalog Number
PA1647
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-5HT1A Receptor/HTR1A Antibody catalog # PA1647. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-5HT1A Receptor/HTR1A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1647)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human 5HT1A Receptor, different from the related rat and mouse sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1647 is reactive to HTR1A in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
46 kDa
Calculated molecular weight
46107 MW
Background of 5-HT1A
HTR1A (5-HYDROXYTRYPTAMINE RECEPTOR 1A), also called SEROTONIN 5-HT-1A RECEPTOR or BETA-2-ADRENERGIC RECEPTOR-LIKE PROTEIN G-21, is a subtype of 5-HT receptor that binds the endogenous neurotransmitter serotonin. It is a G protein-coupled receptor (GPCR) that is coupled to Gi/Go and mediates inhibitory neurotransmission. HTR1A denotes the human gene encoding for the receptor.The HTR1A gene is located at 5q12.3. The decreases in 5-HT-1A receptor densities correlated with decreased glucose utilization as measured by PET scan. Activation of 5-HT-1A receptors has been demonstrated to impair cognition, learning, and memory by inhibiting the release of glutamate and acetylcholine in various areas of the brain. 5-HT-1A receptors in the dorsal raphe nucleus are co-localized with neurokinin 1 (NK1) receptors and have been shown to inhibit the release of substance P, their endogenous ligand.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1647 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Rat, By Heat
Positive Control
WB: human hepatocellular carcinoma tumor tissue (HCCT), rat liver tissue
IHC: human endometrial adenocarcinoma tissue, human lung cancer tissue, rat lymphaden tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of HTR1A using anti-HTR1A antibody (PA1647).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates,
Lane 2: rat liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HTR1A antigen affinity purified polyclonal antibody (Catalog # PA1647) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HTR1A at approximately 46 kDa. The expected band size for HTR1A is at 46 kDa.
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Figure 2. IHC analysis of HTR1A using anti-HTR1A antibody (PA1647).
HTR1A was detected in a paraffin-embedded section of human endometrial adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HTR1A Antibody (PA1647) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 3. IHC analysis of HTR1A using anti-HTR1A antibody (PA1647).
HTR1A was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HTR1A Antibody (PA1647) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of HTR1A using anti-HTR1A antibody (PA1647).
HTR1A was detected in a paraffin-embedded section of rat lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HTR1A Antibody (PA1647) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For HTR1A (Source: Uniprot.org, NCBI)
Gene Name
HTR1A
Full Name
5-hydroxytryptamine receptor 1A
Weight
46107 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
5-hydroxytryptamine receptor 1A;5-HT-1A;5-HT1A;G-21;Serotonin receptor 1A;HTR1A;ADRB2RL1, ADRBRL1; HTR1A 5-HT-1A, 5-HT1A, 5HT1a, ADRB2RL1, ADRBRL1, G-21, PFMCD 5-hydroxytryptamine receptor 1A 5-hydroxytryptamine receptor 1A|5-HT1a receptor|5-hydroxytryptamine (serotonin) receptor 1A, G protein-coupled|guanine nucleotide-binding regulatory protein-coupled receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HTR1A, check out the HTR1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HTR1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-5HT1A Receptor/HTR1A Antibody Picoband® (PA1647)
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Customer Q&As
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7 Customer Q&As for Anti-5HT1A Receptor/HTR1A Antibody Picoband®
Question
Will anti-5HT1A Receptor/HTR1A antibody PA1647 work for WB with brain?
Verified Customer
Verified customer
Asked: 2020-03-26
Answer
According to the expression profile of brain, HTR1A is highly expressed in brain. So, it is likely that anti-5HT1A Receptor/HTR1A antibody PA1647 will work for WB with brain.
Boster Scientific Support
Answered: 2020-03-26
Question
Do you have a BSA free version of anti-5HT1A Receptor/HTR1A antibody PA1647 available?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-5HT1A Receptor/HTR1A antibody PA1647 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-13
Question
Will anti-5HT1A Receptor/HTR1A antibody PA1647 work on goat IHC-P with brain?
Verified Customer
Verified customer
Asked: 2019-12-17
Answer
Our lab technicians have not tested anti-5HT1A Receptor/HTR1A antibody PA1647 on goat. You can run a BLAST between goat and the immunogen sequence of anti-5HT1A Receptor/HTR1A antibody PA1647 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat brain in IHC-P, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-17
Question
We are interested in to test anti-5HT1A Receptor/HTR1A antibody PA1647 on human brain for research purposes, then I may be interested in using anti-5HT1A Receptor/HTR1A antibody PA1647 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-19
Answer
The products we sell, including anti-5HT1A Receptor/HTR1A antibody PA1647, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-19
Question
We are currently using anti-5HT1A Receptor/HTR1A antibody PA1647 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
J. Kulkarni
Verified customer
Asked: 2018-05-21
Answer
The anti-5HT1A Receptor/HTR1A antibody (PA1647) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-21
Question
Is this PA1647 anti-5HT1A Receptor/HTR1A antibody reactive to the isotypes of HTR1A?
Verified Customer
Verified customer
Asked: 2018-04-03
Answer
The immunogen of PA1647 anti-5HT1A Receptor/HTR1A antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human 5HT1A Receptor(404-422aa NKDFQNAFKKIIKCKFCRQ), different from the related rat and mouse sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-04-03
Question
Does PA1647 anti-5HT1A Receptor/HTR1A antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
L. Moore
Verified customer
Asked: 2017-09-12
Answer
You can see on the product datasheet, PA1647 anti-5HT1A Receptor/HTR1A antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-09-12
