VEGF Mouse, Vascular Endothelial Growth Factor Mouse Recombinant Protein, Sf9

Product Name

VEGF Mouse, Vascular Endothelial Growth Factor Mouse Recombinant Protein, Sf9

View all VEGFA recombinant proteins

SKU/Catalog Number

PROTQ00731

Size

2ug, 10ug, 1mg

Description

Vascular Endothelial Growth Factor Mouse Recombinant produced in Sf9 insect cells is a double, glycosylated, polypeptide chain containing 164 amino acids and having a molecular mass of 48 kDa. The VEGF is purified by proprietary chromatographic techniques.

Storage & Handling

Lyophilized Vascular Endothelial Growth Factor Sf9 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGF-Sf9 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Cite This Product

VEGF Mouse, Vascular Endothelial Growth Factor Mouse Recombinant Protein, Sf9 (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTQ00731)

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The protein was lyophilized from a concentrated (1mg/ml) solution with no additives.

Purity

Greater than 95.0% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE.

Predicted MW

27.042kDa

Reconstitution

It is recommended to reconstitute the lyophilized Vascular Endothelial Growth Factor-Sf9 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Biological Activity

The ED₅₀ range, determined by the dose-dependent proliferation of human umbilical vein endothelial cells (HUVEC) (measured by ³H-thymidine uptake) is 1-2 ng/ml, corresponding to a specific activity of 1x10⁶ Units/mg.

Reconstitution

It is recommended to reconstitute the lyophilized Vascular Endothelial Growth Factor-Sf9 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Validation Images & Assay Conditions

Gene/Protein Information For VEGFA (Source: Uniprot.org, NCBI)

Alternative Names

Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF; VEGF; MGC70609 VEGFA MVCD1, VEGF, VPF vascular endothelial growth factor A vascular endothelial growth factor A|vascular endothelial growth factor A121|vascular endothelial growth factor A165|vascular permeability factor

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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