Topoisomerase I (TOP1) (NM_003286) Human Over-expression Lysate

Product Name

Topoisomerase I (TOP1) (NM_003286) Human Over-expression Lysate

See all Topoisomerase I products

SKU/Catalog Number

LS007150

Size

100 µg

Description

Transient overexpression lysate of topoisomerase (DNA) I (TOP1)

Storage & Handling

The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.

Cite This Product

Topoisomerase I (TOP1) (NM_003286) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS007150)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

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Gene/Protein Information for TOP1 (Source: Uniprot.org, NCBI)

Alternative Names

DNA topoisomerase 1; DNA topoisomerase I; EC 5.99.1.2; TOPI; topoisomerase (DNA) I; type I DNA topoisomerase TOP1 TOPI DNA topoisomerase I DNA topoisomerase 1|Scl-70 |topoisomerase (DNA) I|type I DNA topoisomerase

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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