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- Table of Contents
Facts about DNA topoisomerase 1.
The scissile phosphodiester is assaulted by the catalytic tyrosine of the enzyme, leading to the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils.
Human | |
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Gene Name: | TOP1 |
Uniprot: | P11387 |
Entrez: | 7150 |
Belongs to: |
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type IB topoisomerase family |
DNA topoisomerase 1; DNA topoisomerase I; EC 5.99.1.2; TOPI; topoisomerase (DNA) I; type I DNA topoisomerase
Mass (kDA):
90.726 kDA
Human | |
---|---|
Location: | 20q12 |
Sequence: | 20; NC_000020.11 (41028822..41124487) |
Endothelial cells.
Nucleus, nucleolus. Nucleus, nucleoplasm. Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumoylated forms found in both nucleoplasm and nucleoli.
Boster Bio's Anti DNA topoisomerase 1 TOP1 marker is available in various formats. These guides will assist you to improve and troubleshoot your experiments. These guides will help you determine the best way to conduct your experiments, and offer suggestions on how to optimize your experiments. Whatever experiment you're conducting there is always the chance of errors. However the proper monitoring and controls can eliminate many of these risks.
The anti-DNA topoisomerase 1 TOP1 (also known as DNA-TOP1) antibody is available from Boster Bio. This antibody reacts with both Human and Mouse proteins and has been tested for IHC, IP and WB applications. It is also available as an inhibitory peptide. If you aren't sure if this antibody is appropriate for your studies, consult the specifications of the product to ensure compatibility with your specific sample.
DNA topoisomerases I and II catalyze disintegration and unwinding process of DNA. These enzymes are targeted by anti-cancer drugs. For instance yeast type II topoimerase is able to bend a 10-base pair of DNA by 150 degrees. Bacterial Gyrase wraps DNA in 180-degree twists. Both of these actions require significant energy and may represent a rate limiting step in the catalytic cycle. TOP1 and TOP2 have the ability to attach to DNA and hydrolyze ATP during catalysis.
In a study carried out by Kumar V and Blaszczyk M They found that the two antibodies were associated with nucleolar antigens and the cytoplasmic 8-2-ribonucleoproteins present in s sera. These results indicate that this marker could be a useful method for detecting early rheumatoid arthritis as well as systemic scleroderma.
The optimalisation of the Boster Bio TIP1 marker requires careful decision-making at each stage. Every step is vital starting with sample preparation and selecting a buffer that blocks. If you are unsure about an aspect of the ELISA, Boster Bio offers troubleshooting tips and guides to aid you in understanding the procedure. This guide also explains the fundamentals of ELISA as well as its protocols and the application of the TOP1 marker. Boster's technical blog includes articles on various aspects of ELISA, from its basic principles and application to recommended products.
It isn't always easy to determine the cause of a problem in your system. A diagnosis is an excellent place to start. This includes symptoms and other unusual circumstances. This is a step to identify the cause of the issue and identify the best way to reproduce it. The solution will usually involve changing or replacing a component and then testing it. Then, you'll have to repeat the process for each component if it is feasible.
It is vital to identify the root of a computer problem before you can resolve it. Troubleshooting methods are a great method to determine the root source of the issue and return your computer to normal. A checklist can be used to determine the most common causes. Then, you can focus on the most appropriate solution by identifying the cause of the issue. In some cases you might need to consult with an expert in technical support however, it's a worthwhile exercise.
The TOP1 marker is extremely dependable in its purpose. It is used to identify sexes in livestock. The TOP1 technique can be employed in a variety of different species too, including chickens, cattle, pigs as well as humans. CRISPR-Cas9 allows transgenesis of many different genetic variants simple. Cattle, chickens, and pigs all have safe-harbour loci to allow transgene integration. While zygote injection may be an option, additional breeding steps are required.
PMID: 2833744 by D'Arpa P., et al. cDNA cloning of human DNA topoisomerase I: catalytic activity of a 67.7-kDa carboxyl-terminal fragment.
PMID: 1851751 by Kunze N., et al. Structure of the human type I DNA topoisomerase gene.