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Product Info Summary
| SKU: | A00434-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Topoisomerase I/TOP1 Antibody Picoband®
View all Topoisomerase I Antibodies
SKU/Catalog Number
A00434-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Topoisomerase I/TOP1 Antibody Picoband® catalog # A00434-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Topoisomerase I/TOP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00434-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Topoisomerase I recombinant protein (Position: E526-F765).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00434-1 is reactive to TOP1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
100 kDa
Calculated molecular weight
90.726kDa
Background of Topoisomerase I
DNA topoisomerase 1 is an enzyme that in humans is encoded by the TOP1 gene. This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00434-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human HEL whole cell, human Daudi whole cell, human MOLT-4 whole cell, human MCF-7 whole cell, rat brain tissue, mouse brain tissue
ICC/IF: Hela cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Topoisomerase I using anti-Topoisomerase I antibody (A00434-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human Daudi whole cell lysates,
Lane 3: human MOLT-4 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Topoisomerase I antigen affinity purified polyclonal antibody (Catalog # A00434-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Topoisomerase I at approximately 100 kDa. The expected band size for Topoisomerase I is at 91 kDa.
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Figure 2. IF analysis of Topoisomerase I using anti-Topoisomerase I antibody (A00434-1).
Topoisomerase I was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Topoisomerase I Antibody (A00434-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For TOP1 (Source: Uniprot.org, NCBI)
Gene Name
TOP1
Full Name
DNA topoisomerase 1
Weight
90.726kDa
Superfamily
type IB topoisomerase family
Alternative Names
DNA topoisomerase 1; DNA topoisomerase I; TOP1; TOP1 TOPI DNA topoisomerase I DNA topoisomerase 1|Scl-70 |topoisomerase (DNA) I|type I DNA topoisomerase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TOP1, check out the TOP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TOP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Topoisomerase I/TOP1 Antibody Picoband® (A00434-1)
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Customer Q&As
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16 Customer Q&As for Anti-Topoisomerase I/TOP1 Antibody Picoband®
Question
Is a blocking peptide available for product anti-Topoisomerase I/TOP1 antibody (A00434-1)?
A. Edwards
Verified customer
Asked: 2020-04-14
Answer
We do provide the blocking peptide for product anti-Topoisomerase I/TOP1 antibody (A00434-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-14
Question
Is this A00434-1 anti-Topoisomerase I/TOP1 antibody reactive to the isotypes of TOP1?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
The immunogen of A00434-1 anti-Topoisomerase I/TOP1 antibody is E. coli-derived human Topoisomerase I recombinant protein (Position: E526-F765). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-10
Question
Would anti-Topoisomerase I/TOP1 antibody A00434-1 work for WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
According to the expression profile of cervix carcinoma erythroleukemia, TOP1 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-Topoisomerase I/TOP1 antibody A00434-1 will work for WB with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2019-09-16
Question
I see that the anti-Topoisomerase I/TOP1 antibody A00434-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-16
Question
We ordered your anti-Topoisomerase I/TOP1 antibody for WB on cervix carcinoma erythroleukemia in the past. I am using rat, and I plan to use the antibody for ELISA next. I am looking for examining cervix carcinoma erythroleukemia as well as cervix carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
I looked at the website and datasheets of our anti-Topoisomerase I/TOP1 antibody and it seems that A00434-1 has been validated on rat in both WB and ELISA. Thus A00434-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-06-21
Question
Do you have a BSA free version of anti-Topoisomerase I/TOP1 antibody A00434-1 available?
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Topoisomerase I/TOP1 antibody A00434-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-14
Question
My question regarding product A00434-1, anti-Topoisomerase I/TOP1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
C. Jha
Verified customer
Asked: 2018-04-27
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00434-1 anti-Topoisomerase I/TOP1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-04-27
Question
We are interested in using your anti-Topoisomerase I/TOP1 antibody for dna replication studies. Has this antibody been tested with western blotting on a431 whole cell lysates? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-02-16
Answer
Thanks for your inquiry. This A00434-1 anti-Topoisomerase I/TOP1 antibody is validated on human hela, hela whole cell lysates, hepg2 whole cell lysates, a431 whole cell lysates, rat testis tissue, mouse testis tissue. It is guaranteed to work for ELISA, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-02-16
Question
I was wanting to use your anti-Topoisomerase I/TOP1 antibody for WB for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2018-01-23
Answer
It shows on the product datasheet, A00434-1 anti-Topoisomerase I/TOP1 antibody has been validated for ELISA, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-23
Question
Our lab were happy with the WB result of your anti-Topoisomerase I/TOP1 antibody. However we have observed positive staining in peripheral blood nucleolus using this antibody. Is that expected? Could you tell me where is TOP1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-10-09
Answer
According to literature, peripheral blood does express TOP1. Generally TOP1 expresses in nucleus, nucleolus. Regarding which tissues have TOP1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Peripheral blood, Pubmed ID: 7882333, 2461859
Trachea, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-10-09
Question
We are currently using anti-Topoisomerase I/TOP1 antibody A00434-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2017-07-24
Answer
The anti-Topoisomerase I/TOP1 antibody (A00434-1) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-24
Question
We need to test anti-Topoisomerase I/TOP1 antibody A00434-1 on human cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-Topoisomerase I/TOP1 antibody A00434-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
R. Jha
Verified customer
Asked: 2016-12-29
Answer
The products we sell, including anti-Topoisomerase I/TOP1 antibody A00434-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-12-29
Question
Would A00434-1 anti-Topoisomerase I/TOP1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
L. Huang
Verified customer
Asked: 2015-03-23
Answer
You can see on the product datasheet, A00434-1 anti-Topoisomerase I/TOP1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-03-23
Question
See below the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Topoisomerase I/TOP1 antibody A00434-1. Please let me know if you require anything else.
K. Anderson
Verified customer
Asked: 2015-01-14
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-01-14
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Topoisomerase I/TOP1 antibody A00434-1. Let me know if you need anything else.
M. Banerjee
Verified customer
Asked: 2014-08-22
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-08-22
Question
We have observed staining in mouse cervix carcinoma. Do you have any suggestions? Is anti-Topoisomerase I/TOP1 antibody supposed to stain cervix carcinoma positively?
K. Moore
Verified customer
Asked: 2013-10-04
Answer
According to literature cervix carcinoma does express TOP1. According to Uniprot.org, TOP1 is expressed in trabecular bone tissue, trachea, ovarian carcinoma, peripheral blood, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have TOP1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Peripheral blood, Pubmed ID: 7882333, 2461859
Trachea, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2013-10-04
