Product Info Summary
SKU: | M00434 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-TOP1/Topoisomerase I Rabbit Monoclonal Antibody
View all Topoisomerase I Antibodies
SKU/Catalog Number
M00434
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-TOP1/Topoisomerase I Rabbit Monoclonal Antibody catalog # M00434. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TOP1/Topoisomerase I Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00434)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FIG-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human TOP1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00434 is reactive to TOP1 in Human, Mouse
Applications
M00434 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
160-180 kDa
Calculated molecular weight
90726 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
![M00434 TOP1 primary antibodies WB testing 1 M00434 TOP1 primary antibodies WB testing 1](https://www.bosterbio.com/media/catalog/product/M/0/M00434-TOP1-primary-antibodies-WB-testing-1.jpg)
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Western blot analysis of TOP1 expression in Jurkat cell lysate.
![m00434 top1 primary antibodies ihc testing 2 m00434 top1 primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/m/0/m00434-top1-primary-antibodies-ihc-testing-2.jpg)
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Immunohistochemical analysis of paraffin-embedded human colon, using TOP1 Antibody.
![m00434 top1 primary antibodies ihc testing 3 m00434 top1 primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/m/0/m00434-top1-primary-antibodies-ihc-testing-3.jpg)
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Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Topoisomerase I antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
![m00434 wb7 m00434 wb7](https://www.bosterbio.com/media/catalog/product/m/0/m00434-wb7.jpg)
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All lanes use the Antibody at 1:3W dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For TOP1 (Source: Uniprot.org, NCBI)
Gene Name
TOP1
Full Name
DNA topoisomerase 1
Weight
90726 MW
Superfamily
type IB topoisomerase family
Alternative Names
DNA topoisomerase 1; DNA topoisomerase I; EC 5.99.1.2; TOPI; topoisomerase (DNA) I; type I DNA topoisomerase TOP1 TOPI DNA topoisomerase I DNA topoisomerase 1|Scl-70 antigen|topoisomerase (DNA) I|type I DNA topoisomerase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TOP1, check out the TOP1 Infographic
![TOP1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TOP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TOP1/Topoisomerase I Rabbit Monoclonal Antibody (M00434)
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No publications found for M00434
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Customer Q&As
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17 Customer Q&As for Anti-TOP1/Topoisomerase I Rabbit Monoclonal Antibody
Question
My colleagues were happy with the WB result of your anti-TOP1/Topoisomerase I Rabbit Monoclonal antibody. However we have seen positive staining in peripheral blood nucleus using this antibody. Is that expected? Could you tell me where is TOP1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
Based on literature, peripheral blood does express TOP1. Generally TOP1 expresses in nucleus, nucleolus. Regarding which tissues have TOP1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Peripheral blood, Pubmed ID: 7882333, 2461859
Trachea, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-03-18
Question
We have observed staining in human liver. What should we do? Is anti-TOP1/Topoisomerase I Rabbit Monoclonal antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
According to literature liver does express TOP1. According to Uniprot.org, TOP1 is expressed in trabecular bone tissue, trachea, ovarian carcinoma, peripheral blood, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have TOP1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Peripheral blood, Pubmed ID: 7882333, 2461859
Trachea, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-02-21
Question
I was wanting to use your anti-TOP1/Topoisomerase I Rabbit Monoclonal antibody for WB for mouse liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse liver identification?
J. Mangal
Verified customer
Asked: 2020-02-11
Answer
As indicated on the product datasheet, M00434 anti-TOP1/Topoisomerase I Rabbit Monoclonal antibody has been tested for IF, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-11
Question
Will anti-TOP1/Topoisomerase I Rabbit Monoclonal antibody M00434 work on primate IF with trabecular bone tissue?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
Our lab technicians have not tested anti-TOP1/Topoisomerase I Rabbit Monoclonal antibody M00434 on primate. You can run a BLAST between primate and the immunogen sequence of anti-TOP1/Topoisomerase I Rabbit Monoclonal antibody M00434 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate trabecular bone tissue in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-13
Question
Will M00434 anti-TOP1/Topoisomerase I Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
As indicated on the product datasheet, M00434 anti-TOP1/Topoisomerase I Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-15
Question
We are currently using anti-TOP1/Topoisomerase I Rabbit Monoclonal antibody M00434 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
The anti-TOP1/Topoisomerase I Rabbit Monoclonal antibody (M00434) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-28
Question
Here is the WB image, lot number and protocol we used for liver using anti-TOP1/Topoisomerase I Rabbit Monoclonal antibody M00434. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-11
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-TOP1/Topoisomerase I Rabbit Monoclonal antibody M00434. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-16
Question
We ordered your anti-TOP1/Topoisomerase I Rabbit Monoclonal antibody for IF on liver in the past. I am using human, and I plan to use the antibody for WB next. I was wanting to use examining liver as well as cervix carcinoma in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
I took a look at the website and datasheets of our anti-TOP1/Topoisomerase I Rabbit Monoclonal antibody and I see that M00434 has been validated on human in both IF and WB. Thus M00434 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-06-13
Question
Will anti-TOP1/Topoisomerase I Rabbit Monoclonal antibody M00434 work for WB with liver?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
According to the expression profile of liver, TOP1 is highly expressed in liver. So, it is likely that anti-TOP1/Topoisomerase I Rabbit Monoclonal antibody M00434 will work for WB with liver.
Boster Scientific Support
Answered: 2019-06-11
Question
I would like using your anti-TOP1/Topoisomerase I Rabbit Monoclonal antibody for dna replication studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-01-16
Answer
We appreciate your inquiry. This M00434 anti-TOP1/Topoisomerase I Rabbit Monoclonal antibody is validated on jurkat cell lysate. It is guaranteed to work for IF, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-01-16
Question
Do you have a BSA free version of anti-TOP1/Topoisomerase I Rabbit Monoclonal antibody M00434 available?
Verified Customer
Verified customer
Asked: 2017-12-28
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-TOP1/Topoisomerase I Rabbit Monoclonal antibody M00434 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-12-28
Question
Is a blocking peptide available for product anti-TOP1/Topoisomerase I Rabbit Monoclonal antibody (M00434)?
D. Jha
Verified customer
Asked: 2017-12-26
Answer
We do provide the blocking peptide for product anti-TOP1/Topoisomerase I Rabbit Monoclonal antibody (M00434). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-12-26
Question
My lab would like to test anti-TOP1/Topoisomerase I Rabbit Monoclonal antibody M00434 on mouse liver for research purposes, then I may be interested in using anti-TOP1/Topoisomerase I Rabbit Monoclonal antibody M00434 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-05-30
Answer
The products we sell, including anti-TOP1/Topoisomerase I Rabbit Monoclonal antibody M00434, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-05-30
Question
Is this M00434 anti-TOP1/Topoisomerase I Rabbit Monoclonal antibody reactive to the isotypes of TOP1?
M. Moore
Verified customer
Asked: 2015-02-20
Answer
The immunogen of M00434 anti-TOP1/Topoisomerase I Rabbit Monoclonal antibody is A synthesized peptide derived from human TOP1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-02-20
Question
I have a question about product M00434, anti-TOP1/Topoisomerase I Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Bhatt
Verified customer
Asked: 2013-11-29
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00434 anti-TOP1/Topoisomerase I Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-11-29
Question
I see that the anti-TOP1/Topoisomerase I Rabbit Monoclonal antibody M00434 works with WB, what is the protocol used to produce the result images on the product page?
W. Miller
Verified customer
Asked: 2013-05-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-05-17