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Key=entity_id, value=55018
Key=attribute_set_id, value=4
Key=type_id, value=simple
Key=sku, value=EKC2582
Key=has_options, value=0
Key=required_options, value=0
Key=created_at, value=2019-02-12 00:21:02
Key=updated_at, value=2024-09-26 01:02:09
Key=mst_search_weight, value=0
Key=status, value=Enabled
Key=name, value=RIPK2 (Phospho-Ser176) Colorimetric Cell-Based ELISA Kit
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Key=price, value=540.0000
Key=weight, value=1.0000
Key=meta_title, value=RIPK2 (Phospho-Ser176) Colorimetric Cell-Based ELISA Kit Price
Key=meta_description, value=RIPK2 (Phospho-Ser176) Colorimetric Cell-Based ELISA Kit EKC2582
Key=image, value=/b/o/boster-box.png
Key=small_image, value=/b/o/boster-box.png
Key=thumbnail, value=/b/o/boster-box.png
Key=url_key, value=ripk2-phospho-ser176-colorimetric-cell-based-elisa-kit-ekc2582-boster
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Key=image_label, value=Boster Kit Box
Key=small_image_label, value=Boster Kit Box
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Key=size, value=1 kit, containing two 96-well plates and all necessary reagents
Key=gene_full_name, value=Receptor-interacting serine/threonine-protein kinase 2
Key=uniprot_id, value=O43353
Key=assay_range, value=> 5000 cells/well
Key=sensitivity
Key=applications, value=ELISA
Key=reactivity, value=Human, Mouse
Key=source_company, value=AssayBiotech CBP1771; Cost: 300
Key=molecular_weight, value=61195 MW
Key=product_category, value=Cell-Based ELISA Kits
Key=rating_value, value=95
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Key=special_from_date
Key=description, value=The RIPK2 (Phospho-Ser176) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor RIPK2 (Phospho-Ser176) protein expression profile in cells. The kit can be used for measuring the relative amounts of RIPK2 (Phospho-Ser176) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on RIPK2 (Phospho-Ser176).
Key=short_description, value=The RIPK2 (Phospho-Ser176) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor RIPK2 (Phospho-Ser176) protein expression profile in cells. The kit can be used for measuring the relative amounts of RIPK2 (Phospho-Ser176) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on RIPK2 (Phospho-Ser176).
Key=gene_name, value=RIPK2UNQ277/PRO314/PRO34092
Key=synonyms, value=Receptor-interacting serine/threonine-protein kinase 2;2.7.11.1;CARD-containing interleukin-1 beta-converting enzyme-associated kinase;CARD-containing IL-1 beta ICE-kinase;RIP-like-interacting CLARP kinase;Receptor-interacting protein 2;RIP-2;Tyrosine-protein kinase RIPK2;2.7.10.2;RIPK2;CARDIAK, RICK, RIP2;UNQ277/PRO314/PRO34092;
Key=kit_components, value=RIPK2 (Phospho-Ser176)
Key=subcellular_localization, value=Cytoplasm.
Key=tissue_specificity, value=Detected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node.
Key=protein_function, value= Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation.
Key=storage, value=Store at 4°C for up to 6 months.
Key=custom_attribute_1, value=RIPK2
Key=custom_attribute_2, value=Phospho-Ser176
Key=options
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Key=extension_attributes
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Key=tier_price_changed
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RIPK2 (Phospho-Ser176) Colorimetric Cell-Based ELISA Kit
RIPK2 ELISA kit, phospho-specific, cell-based
The RIPK2 (Phospho-Ser176) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor RIPK2 (Phospho-Ser176) protein expression profile in cells. The kit can be used for measuring the relative amounts of RIPK2 (Phospho-Ser176) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on RIPK2 (Phospho-Ser176).
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RIPK2 (Phospho-Ser176) Colorimetric Cell-Based ELISA Kit Validation Images Gallery
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Product Info Summary
SKU:
EKC2582
Size:
1 kit, containing two 96-well plates and all necessary reagents
Reactive Species:
Human, Mouse
Application:
ELISA
Product Name
RIPK2 (Phospho-Ser176) Colorimetric Cell-Based ELISA Kit
See all RIPK2 products
SKU/Catalog Number
EKC2582
Size
1 kit, containing two 96-well plates and all necessary reagents
Description
The RIPK2 (Phospho-Ser176) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor RIPK2 (Phospho-Ser176) protein expression profile in cells. The kit can be used for measuring the relative amounts of RIPK2 (Phospho-Ser176) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on RIPK2 (Phospho-Ser176).
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
RIPK2 (Phospho-Ser176) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2582)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC2582 is reactive to RIPK2UNQ277/PRO314/PRO34092 in Human, Mouse samples
Application Guarantee
EKC2582 is guaranteed for ELISA in Human, Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
Reagent
Quantity
Container
96-Well Cell Culture Clear-Bottom Microplate
2 Plates
-
10x TBS
24 ml (10x)
Clear
Quenching Buffer
24 ml (1x)
Clear
Blocking Buffer
50 ml (1x)
Clear
15x Wash Buffer
50 ml (15x)
Clear
100x Anti-RIPK2 Phospho-Ser176 Antibody (Rabbit Polyclonal)
60 µl (100x)
Red
100x Anti-RIPK2 Antibody (Rabbit Polyclonal)
60 µl (100x)
Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal)
60 µl (100x)
Green
HRP-Conjugated Anti-Rabbit IgG Antibody
12ml (1x)
Glass
HRP-Conjugated Anti-Mouse IgG Antibody
12ml (1x)
Glass
Primary Antibody Diluent
12 ml (1x)
Clear
Ready-to-Use Substrate
12 ml (1x)
Brown
Stop Solution
12 ml (1x)
Clear
Crystal Violet Solution
12ml (1x)
Glass
SDS Solution
24 ml (1x)
Clear
Adhesive Plate Seals
2 Seals
-
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional) Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources Deionized or sterile water Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer Graph paper or computer software capable of generating or displaying logarithmic functions Absorbent papers or vacuum aspirator Test tubes or microfuge tubes capable of storing 1 ml Orbital shaker Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
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Western blot analysis of extracts from 293 cells treated with UV 15'
Gene/Protein Information For RIPK2UNQ277/PRO314/PRO34092 (Source: Uniprot.Org, NCBI)
Gene Name
RIPK2UNQ277/PRO314/PRO34092
Full Name
Receptor-interacting serine/threonine-protein kinase 2
Superfamily
protein kinase superfamily
Alternative Names
Receptor-interacting serine/threonine-protein kinase 2;2.7.11.1;CARD-containing interleukin-1 beta-converting enzyme-associated kinase;CARD-containing IL-1 beta ICE-kinase;RIP-like-interacting CLARP kinase;Receptor-interacting protein 2;RIP-2;Tyrosine-protein kinase RIPK2;2.7.10.2;RIPK2;CARDIAK, RICK, RIP2;UNQ277/PRO314/PRO34092; RIPK2 CARD3, CARDIAK, CCK, GIG30, RICK, RIP2 receptor interacting serine/threonine kinase 2 receptor-interacting serine/threonine-protein kinase 2|CARD-carrying kinase|CARD-containing IL-1 beta ICE-kinase|CARD-containing interleukin-1 beta-converting enzyme (ICE)-associated kinase|RIP-2|growth-inhibiting gene 30|receptor-interacting protein (RIP)-like interacting caspase-like apoptosis regulatory protein (CLARP) kinase|receptor-interacting protein 2|tyrosine-protein kinase RIPK2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on RIPK2UNQ277/PRO314/PRO34092, check out the RIPK2UNQ277/PRO314/PRO34092 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for RIPK2UNQ277/PRO314/PRO34092: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected] .
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