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Key=gene_name, value=RIPK2UNQ277/PRO314/PRO34092
Key=synonyms, value=Receptor-interacting serine/threonine-protein kinase 2;2.7.11.1;CARD-containing interleukin-1 beta-converting enzyme-associated kinase;CARD-containing IL-1 beta ICE-kinase;RIP-like-interacting CLARP kinase;Receptor-interacting protein 2;RIP-2;Tyrosine-protein kinase RIPK2;2.7.10.2;RIPK2;CARDIAK, RICK, RIP2;UNQ277/PRO314/PRO34092;
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Key=subcellular_localization, value=Cytoplasm.
Key=tissue_specificity, value=Detected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node.
Key=protein_function, value= Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation.
Key=storage, value=Store at 4°C for up to 6 months.
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RIPK2 (Phospho-Ser176) Colorimetric Cell-Based ELISA Kit

RIPK2 ELISA kit, phospho-specific, cell-based

The RIPK2 (Phospho-Ser176) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor RIPK2 (Phospho-Ser176) protein expression profile in cells. The kit can be used for measuring the relative amounts of RIPK2 (Phospho-Ser176) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on RIPK2 (Phospho-Ser176).

Product Info Summary

SKU: EKC2582
Size: 1 kit, containing two 96-well plates and all necessary reagents
Reactive Species: Human, Mouse
Application: ELISA

Product Name

RIPK2 (Phospho-Ser176) Colorimetric Cell-Based ELISA Kit

See all RIPK2 products

SKU/Catalog Number

EKC2582

Size

1 kit, containing two 96-well plates and all necessary reagents

Description

The RIPK2 (Phospho-Ser176) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor RIPK2 (Phospho-Ser176) protein expression profile in cells. The kit can be used for measuring the relative amounts of RIPK2 (Phospho-Ser176) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on RIPK2 (Phospho-Ser176).

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

RIPK2 (Phospho-Ser176) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2582)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC2582 is reactive to RIPK2UNQ277/PRO314/PRO34092 in Human, Mouse samples

Application Guarantee

EKC2582 is guaranteed for ELISA in Human, Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 2 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-RIPK2 Phospho-Ser176 Antibody (Rabbit Polyclonal) 60 µl (100x) Red
100x Anti-RIPK2 Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 12ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 12ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 12ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

Gene/Protein Information For RIPK2UNQ277/PRO314/PRO34092 (Source: Uniprot.Org, NCBI)

Gene Name

RIPK2UNQ277/PRO314/PRO34092

Full Name

Receptor-interacting serine/threonine-protein kinase 2

Weight

61195 MW

Superfamily

protein kinase superfamily

Alternative Names

Receptor-interacting serine/threonine-protein kinase 2;2.7.11.1;CARD-containing interleukin-1 beta-converting enzyme-associated kinase;CARD-containing IL-1 beta ICE-kinase;RIP-like-interacting CLARP kinase;Receptor-interacting protein 2;RIP-2;Tyrosine-protein kinase RIPK2;2.7.10.2;RIPK2;CARDIAK, RICK, RIP2;UNQ277/PRO314/PRO34092; RIPK2 CARD3, CARDIAK, CCK, GIG30, RICK, RIP2 receptor interacting serine/threonine kinase 2 receptor-interacting serine/threonine-protein kinase 2|CARD-carrying kinase|CARD-containing IL-1 beta ICE-kinase|CARD-containing interleukin-1 beta-converting enzyme (ICE)-associated kinase|RIP-2|growth-inhibiting gene 30|receptor-interacting protein (RIP)-like interacting caspase-like apoptosis regulatory protein (CLARP) kinase|receptor-interacting protein 2|tyrosine-protein kinase RIPK2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on RIPK2UNQ277/PRO314/PRO34092, check out the RIPK2UNQ277/PRO314/PRO34092 Infographic

RIPK2UNQ277/PRO314/PRO34092 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for RIPK2UNQ277/PRO314/PRO34092: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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