Human sL-Selectin PicoKine® Quick ELISA Kit

Introduction

Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Product Name

Human sL-Selectin PicoKine® Quick ELISA Kit

See all L-Selectin/CD62L products

SKU/Catalog Number

FEK0503

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human sL-Selectin PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human SELL in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Cite This Product

Human sL-Selectin PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0503)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: W39-N332

Sensitivity

<5 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Standard Dilution Instructions

See datasheet of FEK0503 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0503 is reactive to SELL in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate)

Application Guarantee

FEK0503 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of L-Selectin/CD62L

L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17.SELL (L-selectin)is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.1L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point2.

Kit Components

Catalog number	Description	Quantity	Volume
FEK0503-CAP	Anti-tag Pre-coated 96-well Strip Microplate	1	12 strips of 8 wells
EK0503-ST	Human L-Selectin/CD62L Standard	2	10 ng/tube
FEK0503-A	Human SELL Antibody A	1	3ml
FEK0503-B	Human SELL Antibody B	1	3ml
AR1106-1	Sample Diluent	1	15ml
AR1106-7	TBS-T Wash Buffer (25x)	1	12ml
AR1104	Color Developing Reagent (TMB)	1	10ml
AR1105	Stop Solution	1	10ml
PLA-SEA	Adhesive Plate Sealers	2	Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional)
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.
• Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.011	0.262	0.446	0.852	1.492	2.205	2.476	2.617

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human sL-Selectin PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of L-Selectin/CD62L in ELISA kits from four different production batches/lots.

Gene/Protein Information For SELL (Source: Uniprot.Org, NCBI)

Alternative Names

L-selectin;CD62 antigen-like family member L;Leukocyte adhesion molecule 1;LAM-1;Leukocyte surface antigen Leu-8;Leukocyte-endothelial cell adhesion molecule 1;LECAM1;Lymph node homing receptor;TQ1;gp90-MEL;CD62L;SELL;LNHR, LYAM1; SELL CD62L, LAM1, LECAM1, LEU8, LNHR, LSEL, LYAM1, PLNHR, TQ1 selectin L L-selectin|CD62 -like family member L|gp90-MEL|leukocyte surface Leu-8|leukocyte-endothelial cell adhesion molecule 1|lymph node homing receptor|lymphocyte adhesion molecule 1|pln homing receptor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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