Rat MCP-3/CCL7 PicoKine® Quick ELISA Kit

Product Name

Rat MCP-3/CCL7 PicoKine® Quick ELISA Kit

See all CCL7/MCP-3/MARC products

SKU/Catalog Number

FEK1300

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat MCP-3/CCL7 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Rat Ccl7 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Cite This Product

Rat MCP-3/CCL7 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1300)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: E.coli; Immunogen sequence: Q24-P97

Sensitivity

<10 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Standard Dilution Instructions

See datasheet of FEK1300 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK1300 is reactive to CCL7 in Rat samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Application Guarantee

FEK1300 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of CCL7/MCP-3/MARC

Chemokine(C-C motif) ligand 7(CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3(MCP3). It belongs to the C-C chemokine family. By fluorescence in situ hybridization, mapped the MCP3 gene to chromosome 17q11.2-q12. MCP3 was identified as a physiologic substrate of gelatinase A. Cleaved MCP3 binds to CC-chemokine receptors-1, -2, and -3, but no longer induces calcium fluxes or promotes chemotaxis, and instead acts as a general chemokine antagonist that dampens inflammation, suggested that matrix metalloproteinases are both effectors and regulators of the inflammatory response.

Kit Components

Catalog number	Description	Quantity	Volume
FEK1300-CAP	Anti-tag Pre-coated 96-well Strip Microplate	1	12 strips of 8 wells
EK1300-ST	Rat CCL7/MCP-3/MARC Standard	2	10 ng/tube
FEK1300-A	Rat CCL7 Antibody A	1	3ml
FEK1300-B	Rat CCL7 Antibody B	1	3ml
AR1106-1	Sample Diluent	1	15ml
AR1106-7	TBS-T Wash Buffer (25x)	1	12ml
AR1104	Color Developing Reagent (TMB)	1	10ml
AR1105	Stop Solution	1	10ml
PLA-SEA	Adhesive Plate Sealers	2	Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional)
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.
• Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.025	0.171	0.307	0.552	0.946	1.611	2.060	2.226

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat MCP-3/CCL7 PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CCL7/MCP-3/MARC in ELISA kits from four different production batches/lots.

Gene/Protein Information For CCL7 (Source: Uniprot.Org, NCBI)

Alternative Names

C-C motif chemokine 7; CCL7; chemokine (C-C motif) ligand 7; FIC; MARC; MCP-3; MCP3Monocyte chemotactic protein 3; MCP-3Small-inducible cytokine A7; MGC138465; Monocyte chemoattractant protein 3; NC28SCYA6; SCYA7MGC138463; small inducible cytokine A7 (monocyte chemotactic protein 3) CCL7 FIC, MARC, MCP-3, MCP3, NC28, SCYA6, SCYA7 C-C motif chemokine ligand 7 C-C motif chemokine 7|chemokine (C-C motif) ligand 7|monocyte chemoattractant protein 3|monocyte chemotactic protein 3|small inducible cytokine A7 (monocyte chemotactic protein 3)|small-inducible cytokine A7

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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