Rat EPO/Erythropoietin PicoKine® Quick ELISA Kit

Introduction

Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Product Name

Rat EPO/Erythropoietin PicoKine® Quick ELISA Kit

See all Erythropoietin/EPO products

SKU/Catalog Number

FEK1351

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat EPO/Erythropoietin PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Rat Epo in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Rat EPO/Erythropoietin PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1351)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: E.coli; Immunogen sequence: T44-K226

Sensitivity

<10 pg/ml

Assay Range

46.9 pg/ml - 3,000 pg/ml

Standard Dilution Instructions

See datasheet of FEK1351 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK1351 is reactive to Epo in Rat samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin).

Application Guarantee

FEK1351 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Erythropoietin/EPO

Erythropoietin, EPO, also known as hematopoietin or hemopoietin, is a glycoprotein hormone that controls erythropoiesis, or red blood cell production. It is a cytokine for erythrocyte (red blood cell) precursors in the bone marrow. This gene is mapped to 7q22. It is said that the EPO gene encodes a deduced 193-amino acid propolypeptide. This hormone can be found in kidney and liver. It is the hormone that regulates red blood cell production. And it plays an important role in the brain's response to neuronal injury. What’s more, EPO is also involved in the wound healing process.

Kit Components

Catalog number	Description	Quantity
FEK1351-CAP	96-well plate precoated with anti-Rat Erythropoietin/EPO antibody	1
EK1351-ST	lyophilized recombinant Rat Erythropoietin/EPO standard	10 ng/tube
FEK1351-DA	Biotinylated anti-Rat Erythropoietin/EPO detection antibody (50x)	100 ul
-	Avidin-Biotin-Peroxidase Complex (30x)	400 µl
AR1106-1	Sample diluent	30 ml
AR1106-2	Antibody diluent	12 ml
-	Avidin-Biotin-Peroxidase Diluent	12 ml
AR1106-5	Wash Buffer (25x)	20 ml
AR1104	Color Developing Reagent (TMB)	10 ml
AR1105	Stop Solution	10 ml
PLA-SEA	Plate Sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	46.9	93.8	187.5	375	750	1500	3000
O.D.	0.000	0.008	0.016	0.066	0.162	0.449	1.420	2.290

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat EPO/Erythropoietin PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Erythropoietin/EPO in ELISA kits from four different production batches/lots.

Gene/Protein Information For Epo (Source: Uniprot.Org, NCBI)

Alternative Names

Erythropoietin;Epo; EPO DBAL, ECYT5, EP, MVCD2 erythropoietin erythropoietin|epoetin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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