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Product Info Summary
| SKU: | A00484-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-EPO Antibody Picoband®
View all Erythropoietin/EPO Antibodies
SKU/Catalog Number
A00484-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-EPO Antibody Picoband® catalog # A00484-1. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EPO Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00484-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse EPO recombinant protein (Position: D34-R192).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00484-1 is reactive to Epo in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
28 kDa
Calculated molecular weight
52221 MW
Background of Erythropoietin/EPO
This EPO gene encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The encoded protein is mainly synthesized in the kidney, secreted into the blood plasma, and binds to the erythropoietin receptor to promote red blood cell production, or erythropoiesis, in the bone marrow. Expression of this gene is upregulated under hypoxic conditions, in turn leading to increased erythropoiesis and enhanced oxygen-carrying capacity of the blood. Expression of this gene has also been observed in brain and in the eye, and elevated expression levels have been observed in diabetic retinopathy and ocular hypertension. Recombinant forms of the encoded protein exhibit neuroprotective activity against a variety of potential brain injuries, as well as antiapoptotic functions in several tissue types, and have been used in the treatment of anemia and to enhance the efficacy of cancer therapies.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00484-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Positive Control
WB: mouse small intestine tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of EPO using anti-EPO antibody (A00484-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse small intestine tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPO antigen affinity purified polyclonal antibody (Catalog # A00484-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPO at approximately 28KD. The expected band size for EPO is at 21KD.
Protein Target Info & Infographic
Gene/Protein Information For Epo (Source: Uniprot.org, NCBI)
Gene Name
Epo
Full Name
Erythropoietin
Weight
52221 MW
Superfamily
EPO/TPO family
Alternative Names
Erythropoietin; Epo EPO DBAL, ECYT5, EP, MVCD2 erythropoietin erythropoietin|epoetin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Epo, check out the Epo Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Epo: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EPO Antibody Picoband® (A00484-1)
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Customer Q&As
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16 Customer Q&As for Anti-EPO Antibody Picoband®
Question
I was wanting to use your anti-EPO antibody for ELISA for rat urine on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat urine identification?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
You can see on the product datasheet, A00484-1 anti-EPO antibody has been tested for ELISA, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat urine in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-30
Question
I have a question about product A00484-1, anti-EPO antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-08
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00484-1 anti-EPO antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-08
Question
We are currently using anti-EPO antibody A00484-1 for rat tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-26
Answer
The anti-EPO antibody (A00484-1) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-26
Question
Our lab used your anti-EPO antibody for ELISA on right lobe of liver in the past. I am using mouse, and We intend to use the antibody for WB next. Our lab want to know about examining right lobe of liver as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
W. Thomas
Verified customer
Asked: 2020-01-27
Answer
I viewed the website and datasheets of our anti-EPO antibody and it seems that A00484-1 has been tested on mouse in both ELISA and WB. Thus A00484-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-01-27
Question
Does anti-EPO antibody A00484-1 work for ELISA with urine?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
According to the expression profile of urine, EPO is highly expressed in urine. So, it is likely that anti-EPO antibody A00484-1 will work for ELISA with urine.
Boster Scientific Support
Answered: 2020-01-17
Question
Will A00484-1 anti-EPO antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
It shows on the product datasheet, A00484-1 anti-EPO antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-18
Question
I see that the anti-EPO antibody A00484-1 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-12
Question
See below the WB image, lot number and protocol we used for urine using anti-EPO antibody A00484-1. Please let me know if you require anything else.
S. Carter
Verified customer
Asked: 2019-10-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-22
Question
We have seen staining in mouse urine. Any tips? Is anti-EPO antibody supposed to stain urine positively?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
From what I have seen in literature urine does express EPO. From what I have seen in Uniprot.org, EPO is expressed in right lobe of liver, brain, urine, among other tissues. Regarding which tissues have EPO expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Urine, Pubmed ID: 3949763, 6698989, 3346214, 3219367
Boster Scientific Support
Answered: 2019-08-09
Question
My team were happy with the WB result of your anti-EPO antibody. However we have seen positive staining in right lobe of liver secreted. using this antibody. Is that expected? Could you tell me where is EPO supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
From what I have seen in literature, right lobe of liver does express EPO. Generally EPO expresses in secreted. Regarding which tissues have EPO expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Urine, Pubmed ID: 3949763, 6698989, 3346214, 3219367
Boster Scientific Support
Answered: 2019-07-22
Question
Is this A00484-1 anti-EPO antibody reactive to the isotypes of EPO?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
The immunogen of A00484-1 anti-EPO antibody is E. coli-derived mouse EPO recombinant protein (Position: D34-R192). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-09
Question
My lab would like to test anti-EPO antibody A00484-1 on rat urine for research purposes, then I may be interested in using anti-EPO antibody A00484-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Johnson
Verified customer
Asked: 2018-02-13
Answer
The products we sell, including anti-EPO antibody A00484-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-02-13
Question
I was wanting to use using your anti-EPO antibody for embryo implantation studies. Has this antibody been tested with western blotting on mouse small intestine tissue lysates? We would like to see some validation images before ordering.
N. Jha
Verified customer
Asked: 2015-12-02
Answer
I appreciate your inquiry. This A00484-1 anti-EPO antibody is tested on mouse small intestine tissue lysates. It is guaranteed to work for ELISA, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-12-02
Question
Is there a BSA free version of anti-EPO antibody A00484-1 available?
P. Williams
Verified customer
Asked: 2014-12-09
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-EPO antibody A00484-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-12-09
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for urine using anti-EPO antibody A00484-1. Let me know if you need anything else.
C. Moore
Verified customer
Asked: 2013-02-06
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-02-06
Question
Is a blocking peptide available for product anti-EPO antibody (A00484-1)?
L. Miller
Verified customer
Asked: 2013-01-18
Answer
We do provide the blocking peptide for product anti-EPO antibody (A00484-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-01-18
