Anti-Zebrafish Ush2a Antibody

Product Name

Anti-Zebrafish Ush2a Antibody

View all USH2A Antibodies

SKU/Catalog Number

DZ01481

Size

200 μl/vial

Form

Liquid

Description

Boster Bio Polyclonal Anti-Ush2a Antibody catalog # DZ01481. This antibody reacts with Zebrafish.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Zebrafish Ush2a Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # DZ01481)

Host

Rabbit

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

DZ01481 is reactive to USH2A in Zebrafish

Applications

DZ01481 is guaranteed for Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Validation Images & Assay Conditions

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Figure 1. IF analysis of Ush2a using anti-Ush2a antibody (DZ01481).
Ush2a was detected in cryosections of wildtype zebrafish retina tissue without prior fixation. The tissue section was blocked with Blocking buffer (10% NGS, 2% BSA, 0.1% Tween in PBS) for 30 minutes. The tissue section was then incubated with rabbit anti-Ush2a Antibody (DZ01481) in blocking solution at 1:200 dilution overnight at 4°C. Alexa Fluor® 568 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody a and incubated for 1 hour at room temperature in blocking solution. Post immuno-fixation with 4% PFA in PBS for 10 minutes. Mount in Prolong gold.

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Figure 2. IF analysis of Ush2a using anti-Ush2a antibody (DZ01481).
Ush2a was detected in cryosections of Ush2a mutant zebrafish retina tissue without prior fixation. The tissue section was blocked with Blocking buffer (10% NGS, 2% BSA, 0.1% Tween in PBS) for 30 minutes. The tissue section was then incubated with rabbit anti-Ush2a Antibody (DZ01481) in blocking solution at 1:200 dilution overnight at 4°C. Alexa Fluor® 568 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody a and incubated for 1 hour at room temperature in blocking solution. Post immuno-fixation with 4% PFA in PBS for 10 minutes. Mount in Prolong gold.

Gene/Protein Information For USH2A (Source: Uniprot.org, NCBI)

Alternative Names

Usherin USH2A RP39, US2, USH2, dJ1111A8.1 usherin usherin|Usher syndrome 2A (autosomal recessive, mild)|usher syndrome type IIa protein|usher syndrome type-2A protein

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