Product Info Summary
SKU: | EKC1996 |
---|---|
Size: | 1 kit, containing two 96-well plates and all necessary reagents |
Reactive Species: | Human, Mouse, Rat |
Application: | ELISA |
Sample Types: | Cell lines |
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Product info
Product Name
POLR2A (Phospho-Ser1619) Colorimetric Cell-Based ELISA Kit
See all RNA Polymerase II/POLR2A products
SKU/Catalog Number
EKC1996
Size
1 kit, containing two 96-well plates and all necessary reagents
Description
The POLR2A (Phospho-Ser1619) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor POLR2A (Phospho-Ser1619) protein expression profile in cells. The kit can be used for measuring the relative amounts of POLR2A (Phospho-Ser1619) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on POLR2A (Phospho-Ser1619).
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
POLR2A (Phospho-Ser1619) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1996)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC1996 is reactive to POLR2A in Human, Mouse, Rat samples
Validated Sample Types
Cell lines
Application Guarantee
EKC1996 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
Reagent | Quantity | Container |
---|---|---|
96-Well Cell Culture Clear-Bottom Microplate | 2 Plates | - |
10x TBS | 24 ml (10x) | Clear |
Quenching Buffer | 24 ml (1x) | Clear |
Blocking Buffer | 50 ml (1x) | Clear |
15x Wash Buffer | 50 ml (15x) | Clear |
100x Anti-POLR2A Phospho-Ser1619 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Red |
100x Anti-POLR2A Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
HRP-Conjugated Anti-Rabbit IgG Antibody | 12ml (1x) | Glass |
HRP-Conjugated Anti-Mouse IgG Antibody | 12ml (1x) | Glass |
Primary Antibody Diluent | 12 ml (1x) | Clear |
Ready-to-Use Substrate | 12 ml (1x) | Brown |
Stop Solution | 12 ml (1x) | Clear |
Crystal Violet Solution | 12ml (1x) | Glass |
SDS Solution | 24 ml (1x) | Clear |
Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
Click image to see more details
Boster Kit Box
Click image to see more details
Western blot analysis of extracts from COS7 cells treated with EGF 200ng/ml 30'
Protein Target Info & Infographic
Gene/Protein Information For POLR2A (Source: Uniprot.Org, NCBI)
Gene Name
POLR2A
Full Name
DNA-directed RNA polymerase II subunit RPB1
Weight
217176 MW
Superfamily
RNA polymerase beta' chain family
Alternative Names
DNA-directed RNA polymerase II largest subunit;RNA pol II largest subunit;RNA polymerase II 220 kd subunit;RPB1 POLR2A NEDHIB, POLR2, POLRA, RPB1, RPBh1, RPO2, RPOL2, RpIILS, hRPB220, hsRPB1 RNA polymerase II subunit A DNA-directed RNA polymerase II subunit RPB1|DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit|DNA-directed RNA polymerase II subunit A|DNA-directed RNA polymerase III largest subunit|RNA polymerase II subunit B1|RNA-directed RNA polymerase II subunit RPB1|polymerase (RNA) II (DNA directed) polypeptide A, 220kDa|polymerase (RNA) II subunit A
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on POLR2A, check out the POLR2A Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for POLR2A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For POLR2A (Phospho-Ser1619) Colorimetric Cell-Based ELISA Kit (EKC1996)
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