PLGF-1 Placenta Growth Factor-1 Human Recombinant Protein, Sf9

PGF protein, Human

Placenta Growth Factor-1 Human Recombinant produced in insect cells is a homodimer, glycosylated polypeptide chain containing 2 x 131 amino acids and having a total molecular mass of approximately 34 kDa. The PLGF-1 is purified by proprietary chromatographic techniques.

Product Info Summary

SKU: PROTP49763
Size: 2ug, 10ug, 1mg
Origin Species: Human
Source: Insect cells

Product Name

PLGF-1 Placenta Growth Factor-1 Human Recombinant Protein, Sf9

View all PGF recombinant proteins

SKU/Catalog Number

PROTP49763

Size

2ug, 10ug, 1mg

Description

Placenta Growth Factor-1 Human Recombinant produced in insect cells is a homodimer, glycosylated polypeptide chain containing 2 x 131 amino acids and having a total molecular mass of approximately 34 kDa. The PLGF-1 is purified by proprietary chromatographic techniques.

Storage & Handling

Lyophilized Placenta Growth Factor 1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PLGF1 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.

Cite This Product

PLGF-1 Placenta Growth Factor-1 Human Recombinant Protein, Sf9 (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP49763)

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a concentrated (1mg/ml) solution containing 50mM acetic acid.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Predicted MW

24.789kDa

Reconstitution

It is recommended to reconstitute the lyophilized Placenta Growth Factor 1 in sterile 0.1M acetic acid not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Amino Acid Sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Pro-Ala-Val.

Biological Activity

The ED50 as determined by its ability to bind to immobilized rh-sFlt-1 in a functional ELISA. PlGF-1 human Recombinant can bind to immobilized rh-sFlt-1 (100ng/well) with a linear range at 0.5-10ng/ml, corresponding to a Specific Activity of 1x105-2x106units/mg.

Reconstitution

It is recommended to reconstitute the lyophilized Placenta Growth Factor 1 in sterile 0.1M acetic acid not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Validation Images & Assay Conditions

Gene/Protein Information For PGF (Source: Uniprot.org, NCBI)

Gene Name

PGF

Full Name

Placenta growth factor

Weight

24.789kDa

Superfamily

PDGF/VEGF growth factor family

Alternative Names

PIGF; PGF; PLGF-1 PGF D12S1900L, PIGF, PLGF, PlGF-2, SHGC-10760, PGF placental growth factor placenta growth factor|placental growth factor, vascular endothelial growth factor-related protein

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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Size

Total: $250

SKU:PROTP49763

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