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Product Info Summary
| SKU: | EK0490 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. |
Product info
Product Name
Human PLGF/PGF ELISA Kit PicoKine®
SKU/Catalog Number
EK0490
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human PLGF ELISA Kit PicoKine® (96 Tests). Quantitate Human PGF in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human PLGF/PGF ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0490)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: A21-R149
Sensitivity
<1 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0490 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0490 is reactive to PGF in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.
Application Guarantee
EK0490 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of PGF
The protein, also called PGF, is 149 amino acids long and shares 53% identity with the platelet-derived growth factor-like region of human VPF. And the N-glycosylated PLGF protein is secreted into the medium and that it functions as a dimer1. The PLGF gene is mapped to 14q24-q31. There are 3 isoforms of PGF, designated PGF1, PGF2, and PGF3. Only PGF2 is able to bind heparin. Additionally, PGF regulates inter- and intramolecular cross-talk between the VEGF receptor tyrosine kinases FLT1 and FLK12. It also can stimulate angiogenesis and collateral growth in ischemic heart and limb with at least a comparable efficiency to VEGF3.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0490-CAP | Anti-Human PGF Pre-coated 96-well strip microplate | 1 |
| EK0490-ST | Human PGF Standard | 2 vials, 10 ng/tube |
| EK0490-DA | Human PGF Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.021 | 0.074 | 0.131 | 0.226 | 0.344 | 0.798 | 1.388 | 2.055 |
Data Example Images
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Human PLGF PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect PGF, Dilution ratio of 1:1, concentration in serum and plasma is 0-31 pg/ml..
Some articles we found to cite concentrations of PGF in samples: 24304210 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human PLGF/PGF ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 40 | 221 | 454 | 37 | 215 | 459 |
| Standard deviation | 3.16 | 13.26 | 23.15 | 3.51 | 13.76 | 25.7 |
| CV (%) | 7.9% | 6% | 5.6% | 9.5% | 6.4% | 5.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of PGF in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 40 | 38 | 37 | 42 | 39 | 1.92 | 4.9% |
| Sample 2 | 221 | 250 | 264 | 244 | 244 | 15.51 | 6.3% |
| Sample 3 | 454 | 469 | 445 | 509 | 469 | 24.49 | 5.2% |
Protein Target Info & Infographic
Gene/Protein Information For PGF (Source: Uniprot.Org, NCBI)
Gene Name
PGF
Full Name
Placenta growth factor
Weight
24789 MW
Superfamily
PDGF/VEGF growth factor family
Alternative Names
Placenta growth factor;PlGF;PGF;PGFL, PLGF; PGF D12S1900L, PIGF, PLGF, PlGF-2, SHGC-10760, PGF placental growth factor placenta growth factor|placental growth factor, vascular endothelial growth factor-related protein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on PGF, check out the PGF Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PGF: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human PLGF/PGF ELISA Kit PicoKine® (EK0490)
Hello CJ!
EK0490 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Attenuation of hyperglycemia-induced apoptotic signaling and anti-angiogenic milieu in cultured cytotrophoblast cells
miR-143-3p targeting of ITGA6 suppresses tumour growth and angiogenesis by downregulating PLGF expression via the PI3K / AKT pathway in gallbladder carcinoma
Concentrations of VEGF and PlGF Decrease in Eyes After Intravitreal Conbercept Injection
Liu M,Wang RB,Xing JH,Tang YX.Nested Case–Control Study of Corin Combined with sFlt-1/PLGF in Predicting the Risk of Preeclampsia.Int J Gen Med.2021;14:2313-2320https://doi.org/10.2147/IJGM.S297344
Species: Human
EK0490 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Ratajczak J, Hilkens P, Gervois P, Wolfs E, Jacobs R, Lambrichts I, Bronckaers A. PLoS One. 2016 Dec 9;11(12):e0167807. doi: 10.1371/journal.pone.0167807. eCollection 2016. Angiogenic Capacity of Periodontal Ligament Stem Cells Pretreated with D...
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Customer Reviews
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Customer Q&As
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11 Customer Q&As for Human PLGF/PGF ELISA Kit PicoKine®
Question
Q: can you recommend the dilution ratio of serum samples for detection of PGF in Human serum? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
D. Thomas
Verified customer
Asked: 2020-05-22
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the PGF ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-05-22
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
K. Smith
Verified customer
Asked: 2019-05-13
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-05-13
Question
Is there any value for PLGF (EK0490) in third trimester pregnancy, with or without intra uterine growth restriction? If no can you provide normal range of PLFG in third trimester pregnancy?
Verified customer
Asked: 2019-04-10
Answer
Unfortunately for the Human PLGF ELISA Kit PicoKine™ (EK0490), we do not have any value for PLGF in third trimester pregnancy.
Boster Scientific Support
Answered: 2019-04-12
Question
What is the normal serum range of EK0490?
Verified Customer
Verified customer
Asked: 2019-04-08
Answer
Please see the normal serum range for the Human PLGF ELISA Kit PicoKine™ (EK0490) below; PLGF ( Catlog. No. EK0490), 0-30pg/mL
Boster Scientific Support
Answered: 2019-04-09
Question
Q: how to proceed with the analysis of ELISA data? I measured PGF level in serum.
B. Gandhi
Verified customer
Asked: 2018-12-31
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-12-31
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
B. Lee
Verified customer
Asked: 2018-11-27
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-11-27
Question
Q: how can I thaw whole blood sample for PGF ELISA after freezing?
N. Krishna
Verified customer
Asked: 2018-11-17
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test PGF for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-11-17
Question
Q: What is the optimal O.D. value for PGF ELISA kit? I performed your PGF ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain PGF even though the O.D. values are not very high?
S. Garcia
Verified customer
Asked: 2018-08-31
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this PGF ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of PGF that can be declared/interpreted as positive by the above standard is 1pg/ml.
Boster Scientific Support
Answered: 2018-08-31
Question
Q: Can PGF ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2018-08-08
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-08-08
Question
Q: can I use EDTA plasma as samples in Human PGF Picokine® ELISA Kit (Catalog # EK0490)?
J. Young
Verified customer
Asked: 2017-05-25
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of PGF causing degradation of its protein structure. PGF may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the PGF ELISA, treating samples with different anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2017-05-25
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis PGF using the EK0490 Human PGF Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2016-12-05
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2016-12-05
