PDGF BBHuman, Platelet Derived Growth Factor-BB Human Recombinant Protein, Yeast

Product Name

PDGF BBHuman, Platelet Derived Growth Factor-BB Human Recombinant Protein, Yeast

View all PDGFB recombinant proteins

SKU/Catalog Number

PROTP01127

Size

2ug, 10ug, 1mg

Description

Platelet-Derived Growth Factor BB Human Recombinant is a glycosylated homodimer produced in Saccharomyces cerevisiae, containing 2x109 amino acids and having a molecular mass of 32kDa. PDGF-BB is purified by proprietary chromatographic techniques.

Storage & Handling

Lyophilized Platelet-derived Growth Factor BB although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF BB should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.

Cite This Product

PDGF BBHuman, Platelet Derived Growth Factor-BB Human Recombinant Protein, Yeast (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP01127)

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The PDGF-BB was lyophilized from 0.2 µm filtered solution in 20mM sodium phosphate in absence of any carrier protein.

Purity

Greater than 98.0% as determined by SDS-PAGE.

Predicted MW

27.283kDa

Reconstitution

It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-BB in sterile 18M-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Biological Activity

The ED₅₀, as measured by its ability to stimulate proliferation of mouse 3T3 fibroblasts is typically 1 ng/ml, corresponding to a Specific Activity of 1,000,000IU/mg.

Reconstitution

It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-BB in sterile 18M-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Validation Images & Assay Conditions

Gene/Protein Information For PDGFB (Source: Uniprot.org, NCBI)

Alternative Names

Glioma-derived growth factor; GDGF; Osteosarcoma-derived Growth Factor; ODGF; SIS; SSV; PDGF2; c-sis; FLJ12858; PDGF-BB; PDGF B-chain; Platelet-derived growth factor beta polypeptide; Becaplermin PDGFB IBGC5, PDGF-2, PDGF2, SIS, SSV, c-sis platelet derived growth factor subunit B platelet-derived growth factor subunit B|PDGF subunit B|PDGF, B chain|becaplermin|epididymis secretory sperm binding protein|platelet-derived growth factor 2|platelet-derived growth factor B chain|platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)|platelet-derived growth factor, beta polypeptide (oncogene SIS)|proto-oncogene c-Sis

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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