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Product Info Summary
| SKU: | EKC2104 |
|---|---|
| Size: | 1 kit, containing two 96-well plates and all necessary reagents |
| Reactive Species: | Human, Mouse, Rat |
| Application: | ELISA |
| Sample Types: | Cell lines |
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Product info
Product Name
PDE4D (Phospho-Ser190/53) Colorimetric Cell-Based ELISA Kit
See all Phosphodiesterase 4D products
SKU/Catalog Number
EKC2104
Size
1 kit, containing two 96-well plates and all necessary reagents
Description
The PDE4D (Phospho-Ser190/53) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor PDE4D (Phospho-Ser190/53) protein expression profile in cells. The kit can be used for measuring the relative amounts of PDE4D (Phospho-Ser190/53) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on PDE4D (Phospho-Ser190/53).
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
PDE4D (Phospho-Ser190/53) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2104)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC2104 is reactive to PDE4D in Human, Mouse, Rat samples
Validated Sample Types
Cell lines
Application Guarantee
EKC2104 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 2 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-PDE4D Phospho-Ser190/53 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Red |
| 100x Anti-PDE4D Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 12ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 12ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 12ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from K562 cells treated with H2O2 100uM 30'
Protein Target Info & Infographic
Gene/Protein Information For PDE4D (Source: Uniprot.Org, NCBI)
Gene Name
PDE4D
Full Name
cAMP-specific 3',5'-cyclic phosphodiesterase 4D
Weight
91115 MW
Superfamily
cyclic nucleotide phosphodiesterase family
Alternative Names
DPDE3, PDE43, PDE4D4, cAMP-specific 3',5'-cyclic phosphodiesterase 4D PDE4D ACRDYS2, DPDE3, HSPDE4D, PDE43N2, STRK1, PDE4D phosphodiesterase 4D cAMP-specific 3,5-cyclic phosphodiesterase 4D|cAMP-specific phosphodiesterase PDE4D6|phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila)|testicular tissue protein Li 136
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on PDE4D, check out the PDE4D Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PDE4D: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For PDE4D (Phospho-Ser190/53) Colorimetric Cell-Based ELISA Kit (EKC2104)
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