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Product Info Summary
| SKU: | A01111-Dyl488 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human PDE4D DyLight® 488 conjugated Antibody
View all Phosphodiesterase 4D Antibodies
SKU/Catalog Number
A01111-Dyl488
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human PDE4D DyLight® 488 conjugated Antibody catalog # A01111-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human PDE4D DyLight® 488 conjugated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01111-Dyl488)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human PDE4D recombinant protein (Position: H466-Q709).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01111-Dyl488 is reactive to PDE4D in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
91.115kDa
Background of Phosphodiesterase 4D
cAMP-specific 3',5'-cyclic phosphodiesterase 4D is an enzyme that in humans is encoded by the PDE4D gene. This gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01111-Dyl488 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Validation Images & Assay Conditions
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Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For PDE4D (Source: Uniprot.org, NCBI)
Gene Name
PDE4D
Full Name
cAMP-specific 3',5'-cyclic phosphodiesterase 4D
Weight
91.115kDa
Superfamily
cyclic nucleotide phosphodiesterase family
Alternative Names
cAMP-specific 3',5'-cyclic phosphodiesterase 4D; DPDE3; PDE43; PDE4D; DPDE3 PDE4D ACRDYS2, DPDE3, HSPDE4D, PDE43N2, STRK1, PDE4D phosphodiesterase 4D cAMP-specific 3,5-cyclic phosphodiesterase 4D|cAMP-specific phosphodiesterase PDE4D6|phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila)|testicular tissue protein Li 136
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PDE4D, check out the PDE4D Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PDE4D: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human PDE4D DyLight® 488 conjugated Antibody (A01111-Dyl488)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Human PDE4D DyLight® 488 conjugated Antibody
Question
Do you have a BSA free version of anti-Human PDE4D DyLight® 488 conjugated antibody A01111-Dyl488 available?
J. Baker
Verified customer
Asked: 2020-02-20
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Human PDE4D DyLight® 488 conjugated antibody A01111-Dyl488 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-20
Question
My question regarding product A01111-Dyl488, anti-Human PDE4D DyLight® 488 conjugated antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01111-Dyl488 anti-Human PDE4D DyLight® 488 conjugated antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-30
Question
I was wanting to use your anti-Human PDE4D DyLight® 488 conjugated antibody for Flow Cytometry for human cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
It shows on the product datasheet, A01111-Dyl488 anti-Human PDE4D DyLight® 488 conjugated antibody has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-20
Question
Is this A01111-Dyl488 anti-Human PDE4D DyLight® 488 conjugated antibody reactive to the isotypes of PDE4D?
Verified Customer
Verified customer
Asked: 2017-09-08
Answer
The immunogen of A01111-Dyl488 anti-Human PDE4D DyLight® 488 conjugated antibody is E. coli-derived human PDE4D recombinant protein (Position: H466-Q709). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-09-08
Question
Is a blocking peptide available for product anti-Human PDE4D DyLight® 488 conjugated antibody (A01111-Dyl488)?
J. Anderson
Verified customer
Asked: 2015-10-05
Answer
We do provide the blocking peptide for product anti-Human PDE4D DyLight® 488 conjugated antibody (A01111-Dyl488). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-10-05
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-Human PDE4D DyLight® 488 conjugated antibody A01111-Dyl488. Let me know if you need anything else.
S. Carter
Verified customer
Asked: 2014-10-09
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-10-09
