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Product Info Summary
| SKU: | LS005111 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of proliferating cell nuclear antigen (PCNA), transcript variant 1 |
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Product info
Product Name
PCNA (NM_002592) Human Over-expression Lysate
SKU/Catalog Number
LS005111
Size
100 µg
Description
Transient overexpression lysate of proliferating cell nuclear antigen (PCNA), transcript variant 1
Storage & Handling
The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Cite This Product
PCNA (NM_002592) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS005111)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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PCNA Human Over-expression Lysates NM_002592.
Western blot validation of overexpression lysate (LS005111) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PCNA (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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PCNA Human Over-expression Lysates NM_182649.
Western blot validation of overexpression lysate (LS005111) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PCNA (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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PCNA Human Over-expression Lysates NM_182649.
Western blot validation of overexpression lysate (LS005111) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PCNA (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for PCNA (Source: Uniprot.org, NCBI)
Gene Name
PCNA
Full Name
Proliferating cell nuclear antigen
Weight
Superfamily
PCNA family
Alternative Names
cyclin; DNA polymerase delta auxiliary protein; MGC8367; proliferating cell nuclear antigen PCNA ATLD2 proliferating cell nuclear antigen proliferating cell nuclear antigen|DNA polymerase delta auxiliary protein|cyclin
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on PCNA, check out the PCNA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PCNA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For PCNA (NM_002592) Human Over-expression Lysate (LS005111)
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