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Product Info Summary
| SKU: | EK1411 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin). |
Product info
Product Name
Mouse VEGF-B ELISA Kit PicoKine®
SKU/Catalog Number
EK1411
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse VEGF-B ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Vegfb in cell culture supernatants, serum and plasma (heparin). . Sensitivity: 2pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse VEGF-B ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1411)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: Q20-A207 & Q20-R148
Sensitivity
<2 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1411 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1411 is reactive to VEGFB in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
Application Guarantee
EK1411 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of VEGF-B
Vascular endothelial growth factor B also known as VEGF-B is a protein that, in humans, is encoded by the VEGF-B gene. VEGF-B is a growth factor that belongs to the vascular endothelial growth factor family, of which VEGF-A is the best-known member. In contrast to VEGF-A, VEGF-B plays a less pronounced role in the vascular system: Whereas VEGF-A is important for the formation of blood vessels, such as during development or in pathological conditions, VEGF-B seems to play a role only in the maintenance of newly formed blood vessels during pathological conditions. It also plays an important role on several types of neurons. And it is important for the protection of neurons in the retina and thecerebral cortex during stroke and of motoneurons during motor neuron diseases such as amyotrophic lateral sclerosis.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1411-CAP | Anti-Mouse VEGFB Pre-coated 96-well strip microplate | 1 |
| EK1411-ST | Mouse VEGFB Standard | 2 vials, 10 ng/tube |
| EK1411-DA | Mouse VEGFB Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.033 | 0.116 | 0.169 | 0.279 | 0.441 | 1.028 | 1.655 | 2.069 |
Data Example Images
Click image to see more details
Mouse VEGF-B PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect VEGF-B, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 31.2 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse VEGF-B ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 70 | 247 | 978 | 69 | 246 | 1037 |
| Standard deviation | 4.13 | 20.17 | 48.90 | 4.83 | 20.17 | 59.10 |
| CV (%) | 5.0% | 7.5% | 5.0% | 7.0% | 8.2% | 5.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of VEGF-B in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 70 | 68 | 79 | 79 | 74 | 5.04 | 6.8% |
| Sample 2 | 247 | 261 | 242 | 260 | 252 | 8.2 | 3.2% |
| Sample 3 | 978 | 875 | 882 | 968 | 925 | 47.44 | 5.1% |
Protein Target Info & Infographic
Gene/Protein Information For VEGFB (Source: Uniprot.Org, NCBI)
Gene Name
VEGFB
Full Name
Vascular endothelial growth factor B
Weight
21914 MW
Superfamily
PDGF/VEGF growth factor family
Alternative Names
vascular endothelial growth factor B; VEGFB; VEGF-B; VEGF-related factor; VRFVEGFL VEGFB VEGFL, VRF vascular endothelial growth factor B vascular endothelial growth factor B|VEGF-related factor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on VEGFB, check out the VEGFB Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for VEGFB: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse VEGF-B ELISA Kit PicoKine® (EK1411)
Hello CJ!
EK1411 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhao M, Shi X, Liang J, Miao Y, Xie W, Zhang Y, Li X. Exp Eye Res. 2011 Dec;93(6):921-6. Doi: 10.1016/J.Exer.2011.10.013. Epub 2011 Nov 4. Expression Of Pro- And Anti-Angiogenic Isoforms Of Vegf In The Mouse Model Of Oxygen-Induced Retinopathy.
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Customer Reviews
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Customer Q&As
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9 Customer Q&As for Mouse VEGF-B ELISA Kit PicoKine®
Question
Which isoforms of VEGF-B (VEGF-B167 and/or VEGF-B186) is the kit able to detect?
Verified customer
Asked: 2021-02-26
Answer
The Mouse VEGF-B ELISA Kit PicoKine™ (EK1411) is able to detect both VEGF-B167 and VEGF-B186.
Boster Scientific Support
Answered: 2021-02-26
Question
Are the EK1411 capture and detection antibodies KO validated?
Verified customer
Asked: 2021-02-26
Answer
The capture and detection antibodies in the Mouse VEGF-B ELISA Kit PicoKine™ (EK1411) are not KO validated.
Boster Scientific Support
Answered: 2021-02-26
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
H. Thompson
Verified customer
Asked: 2020-05-07
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-05-07
Question
Does EK1411 recognise both VEGF-B186 and VEGF-B167?
Verified customer
Asked: 2020-04-04
Answer
The Mouse VEGF-B ELISA Kit PicoKine EK1411 recognizes VEGF-B186 only.
Boster Scientific Support
Answered: 2020-04-06
Question
Q: What is the optimal O.D. value for VEGF-B ELISA kit? I used your VEGF-B ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain VEGF-B even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2020-03-08
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this VEGF-B ELISA kit to have sensitivity of 2pg/ml, that means the minimum amount of VEGF-B that can be declared/interpreted as positive by the above standard is 2pg/ml.
Boster Scientific Support
Answered: 2020-03-08
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Y. Li
Verified customer
Asked: 2019-06-01
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-06-01
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2016-06-13
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2016-06-13
Question
Q: which procedure should I follow in order to thaw whole blood sample for VEGF-B ELISA after freezing?
Verified Customer
Verified customer
Asked: 2015-08-21
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test VEGF-B for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2015-08-21
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of VEGF-B in Mouse plasma ? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2015-06-15
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the VEGF-B ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2015-06-15
