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Product Info Summary
| SKU: | A04494-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-VEGFB Antibody Picoband®
SKU/Catalog Number
A04494-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-VEGFB Antibody Picoband® catalog # A04494-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VEGFB Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04494-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human VEGFB recombinant protein (Position: Q25-A138).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04494-1 is reactive to VEGFB in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
29 kDa
Calculated molecular weight
21.602kDa
Background of VEGF-B
Vascular endothelial growth factor B also known as VEGF-B is a protein that, in humans, is encoded by the VEGF-B gene. VEGF-B is a growth factor that belongs to the vascular endothelial growth factor family, of which VEGF-A is the best-known member. In contrast to VEGF-A, VEGF-B plays a less pronounced role in the vascular system: Whereas VEGF-A is important for the formation of blood vessels, such as during development or in pathological conditions, VEGF-B seems to play a role only in the maintenance of newly formed blood vessels during pathological conditions. It also plays an important role on several types of neurons. And it is important for the protection of neurons in the retina and thecerebral cortex during stroke and of motoneurons during motor neuron diseases such as amyotrophic lateral sclerosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04494-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human placenta tissue, rat pancreas tissue, mouse cardiac muscle tissue, mouse skeletal muscle tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of VEGFB using anti-VEGFB antibody (A04494-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: rat pancreas tissue lysates,
Lane 3: mouse cardiac muscle tissue lysates,
Lane 4: mouse skeletal muscle tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGFB antigen affinity purified polyclonal antibody (Catalog # A04494-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGFB at approximately 29KD. The expected band size for VEGFB is at 22KD.
Protein Target Info & Infographic
Gene/Protein Information For VEGFB (Source: Uniprot.org, NCBI)
Gene Name
VEGFB
Full Name
Vascular endothelial growth factor B
Weight
21.602kDa
Superfamily
PDGF/VEGF growth factor family
Alternative Names
Vascular endothelial growth factor B; VEGF-B; VEGF-related factor; VRF; VEGFB; VRF; VEGFB VEGFL, VRF vascular endothelial growth factor B vascular endothelial growth factor B|VEGF-related factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VEGFB, check out the VEGFB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VEGFB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-VEGFB Antibody Picoband® (A04494-1)
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