Click image to see more details
Product Info Summary
| SKU: | EK0842 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human TNFSF11/RANKL ELISA Kit PicoKine®
View all TRANCE/TNFSF11/RANK L ELISA kits
SKU/Catalog Number
EK0842
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human TNFSF11/RANKL ELISA Kit PicoKine® (96 Tests). Quantitate Human TNFSF11 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human TNFSF11/RANKL ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0842)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: G64-D245
Sensitivity
<10 pg/ml
Assay Range
78 pg/ml - 5,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0842 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0842 is reactive to TNFSF11 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0842 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TRANCE/TNFSF11/RANK L
Receptor activator of nuclear factor kappa-B ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), is a protein that in humans is encoded by the TNFSF11 gene. This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This gene is mapped to chromosome13q14.11. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. This gene may play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0842-CAP | Anti-Human TNFSF11 Pre-coated 96-well strip microplate | 1 |
| EK0842-ST | Human TNFSF11 Standard | 2 vials, 10 ng/tube |
| EK0842-DA | Human TNFSF11 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 78 | 156 | 313 | 625 | 1250 | 2500 | 5000 |
| O.D. | 0.034 | 0.139 | 0.151 | 0.262 | 0.453 | 0.783 | 1.331 | 1.926 |
Data Example Images
Click image to see more details
Human TNFSF11/RANKL PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect TRANCE/TNFSF11/RANK L, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 78 pg/ml..
Some articles we found to cite concentrations of TRANCE/TNFSF11/RANK L in samples: 22057136 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human TNFSF11/RANKL ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 115 | 865 | 2242 | 114 | 797 | 2279 |
| Standard deviation | 5.52 | 53.63 | 105.37 | 4.72 | 58.97 | 116.22 |
| CV (%) | 4.8% | 6.2% | 4.7% | 5.2% | 7.4% | 5.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of TRANCE/TNFSF11/RANK L in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 115 | 126 | 117 | 122 | 120 | 4.3 | 3.5% |
| Sample 2 | 865 | 746 | 848 | 804 | 815 | 46.01 | 5.6% |
| Sample 3 | 2242 | 2225 | 2256 | 2223 | 2236 | 13.46 | 0.6% |
Protein Target Info & Infographic
Gene/Protein Information For TNFSF11 (Source: Uniprot.Org, NCBI)
Gene Name
TNFSF11
Full Name
Tumor necrosis factor ligand superfamily member 11
Weight
35478 MW
Superfamily
tumor necrosis factor family
Alternative Names
Tumor necrosis factor ligand superfamily member 11;Osteoclast differentiation factor;ODF;Osteoprotegerin ligand;OPGL;Receptor activator of nuclear factor kappa-B ligand;RANKL;TNF-related activation-induced cytokine;TRANCE;CD254;Tumor necrosis factor ligand superfamily member 11, membrane form;Tumor necrosis factor ligand superfamily member 11, soluble form;TNFSF11;OPGL, RANKL, TRANCE; TNFSF11 CD254, ODF, OPGL, OPTB2, RANKL, TNLG6B, TRANCE, hRANKL2, sOdf TNF superfamily member 11 tumor necrosis factor ligand superfamily member 11|TNF-related activation-induced cytokine|osteoclast differentiation factor|osteoprotegerin ligand|receptor activator of nuclear factor kappa B ligand|tumor necrosis factor (ligand) superfamily, member 11|tumor necrosis factor ligand 6B|tumor necrosis factor superfamily member 11
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TNFSF11, check out the TNFSF11 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TNFSF11: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human TNFSF11/RANKL ELISA Kit PicoKine® (EK0842)
Hello CJ!
EK0842 has been cited in 26 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Nitrogen Containing Bisphosphonates Impair the Release of Bone Homeostasis Mediators and Matrix Production by Human Primary Pre-Osteoblasts
Validity of Osteoprotegerin and Receptor Activator of NF-κB Ligand for the Detection of Bone Metastasis in Breast Cancer
Cajanolactone A from Cajanus cajan Promoted Osteoblast Differentiation in Human Bone Marrow Mesenchymal Stem Cells via Stimulating Wnt/LRP5/β-Catenin Signaling
Inflammatory Response of Human Peripheral Blood Mononuclear Cells and Osteoblasts Incubated With Metallic and Ceramic Submicron Particles
An in vitro 3D bone metastasis model by using a human bone tissue culture and human sex-related cancer cells
OPG / RANK / RANKL signaling axis in patients with type I diabetes: Associations with parathormone and vitamin D
Engineering the early bone metastatic niche through human vascularized immuno bone minitissues
Bioengineering the ameloblastoma tumour to study its effect on bone nodule formation
Osteolytic lesions, cytogenetic features and bone marrow levels of cytokines and chemokines in multiple myeloma patients: Role of chemokine (C-C motif) ligand 20
Gradient coatings of strontium hydroxyapatite/zinc β-tricalcium phosphate as a tool to modulate osteoblast/osteoclast response
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Human TNFSF11/RANKL ELISA Kit PicoKine®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Human TNFSF11/RANKL ELISA Kit PicoKine®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
33 Customer Q&As for Human TNFSF11/RANKL ELISA Kit PicoKine®
Question
The samples are undiluted as the protocol says in EK0842. Is it OK? Do you have any recommendation about the samples?
Verified customer
Asked: 2022-11-04
Answer
For the Human TNFSF11/RANKL ELISA Kit PicoKine® (EK0842), the samples need to be diluted in 1:1 to minimize matrix effect. The dilution ratio can be found in the datasheet. https://www.bosterbio.com/datasheet?sku=EK0842
Boster Scientific Support
Answered: 2022-11-06
Question
What exactly is the difference between Human TRANCE/RANKL/TNFSF11 PicoKine Quick ELISA Kit (FEK0842) versus Human TNFSF11/RANKL ELISA Kit PicoKine (EK0842)?
Verified customer
Asked: 2022-03-08
Answer
The main difference between FEK0842 and EK0842 is that FEK0842 (Quick ELISA) takes 90 minutes to run the assay while EK0842 takes the standard ~3 hours to run the assay. For FEK0842, the incubation times have been shortened while maintaining the quality of the product.
Boster Scientific Support
Answered: 2022-03-08
Question
Q: how can I thaw whole blood sample for TRANCE ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-10-21
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test TRANCE for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-10-21
Question
Q: is it okay to use citrate plasma as samples in Human TRANCE Picokine® ELISA Kit (Catalog # EK0842)?
H. Krishna
Verified customer
Asked: 2020-10-18
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of TRANCE causing degradation of its protein structure. TRANCE may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the TRANCE ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-10-18
Question
Does EK0842 have cross-reactivity with mouse and rat samples? Considering the homology between these species is relatively high and the kit is using polyclonal antibodies, does your comment: "There is no detectable cross-reactivity with other relevant proteins." refer to other human proteins in the sample or does this include cross-reactivity with other species?
Verified customer
Asked: 2020-06-29
Answer
Our lab tested recombinant mouse and rat TNFSF11 protein using Human TNFSF11/RANKL ELISA Kit PicoKine™ (EK0842) and didn't observe any cross-reactivity. "There is no detectable cross-reactivity with other relevant proteins." refer to other human proteins in the sample and cross-reactivity with other species we tested as well.
Boster Scientific Support
Answered: 2020-06-29
Question
Does EK0842 have cross-reactivity with mouse and rat samples? Considering the homology between these species is relatively high and the kit is using polyclonal antibodies, does your comment: "There is no detectable cross-reactivity with other relevant proteins." refer to other human proteins in the sample (this is how I read this) or does this include cross-reactivity with other species?
Verified customer
Asked: 2020-06-29
Answer
Our lab tested recombinant mouse and rat TNFSF11 protein using Human TNFSF11/RANKL ELISA Kit PicoKine™ (EK0842) and didn't observe any cross-reactivity. "There is no detectable cross-reactivity with other relevant proteins." refer to other human proteins in the sample and cross-reactivity with other species we tested as well.
Boster Scientific Support
Answered: 2020-06-30
Question
Q: how to proceed with the analysis of ELISA data? I have obtained TRANCE level in plasma.
V. Gonzalez
Verified customer
Asked: 2020-05-11
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-05-11
Question
Do you have any information whether this kit could detect both cytoplasm and membrane bound RANKL?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
According to the immunogen sequence G64-D245 of this kit, it can only detect extracellular secreted protein.
Boster Scientific Support
Answered: 2019-12-25
Question
We recently used your ELISA kit (EK0842) for detecting RANKL protein from cell lysate. 1. Would you have a relevant protocol/literatures where they looked at cell lysate/membrane bound RANKL? 2. We were wondering if you would recommend using protease inhibitor for this type of ELISA? 3. We lysed our samples in deionised H2O by freeze-thaw cycle, could you check if this step is suitable? 4. We were thinking if we should centrifuge the cell lysate before we perform the ELISA? Please could you clarify these steps?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
Please see the following answers for the questions. 1. Would you have a relevant protocol/literatures where they looked at cell lysate/membrane bound RANKL? Preparation of lysate from cell culture (Protein Extraction from Cultured Cells) 1. Rinse cells in ice-cold PBS. 2. Scrape cells or trypsinize cells and wash with PBS. 3. Centrifuge at ~3000 x g for 2-3 minutes, remove PBS. The centrifugation force and time can be varied depending on the cell type. 4. Add IP lysis buffer(Boster Cat#AR0107). 5. Sonicate for 10
Boster Scientific Support
Answered: 2019-12-16
Question
When performing initial estimation of sample concentration (page 10 protocol) for EK0842, how should the remaining empty wells be protected while going through the steps of the protocol?
Verified customer
Asked: 2019-12-04
Answer
For the Human TNFSF11/RANKL ELISA Kit PicoKine™ (EK0842), you can strip off the plate and use the required number of wells only. Meanwhile I can follow up with our labs and address your question about protecting the empty wells.
Boster Scientific Support
Answered: 2019-12-04
Question
I want to know whether your product EK0842 is from human or mouse?
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
The product EK0842 is a full sequence from human.
Boster Scientific Support
Answered: 2019-11-27
Question
What is the host for the capture and detection antibody for EK0842?
Verified customer
Asked: 2019-10-03
Answer
The host for the capture and detection antibody for the Human TNFSF11/RANKL ELISA Kit PicoKine™ (EK0842) is goat polyclonal.
Boster Scientific Support
Answered: 2019-10-03
Question
What is the capture purification method for EK0842?
Verified customer
Asked: 2019-10-03
Answer
For the Human TNFSF11/RANKL ELISA Kit PicoKine™ (EK0842), the capture purification is Affinity Purified.
Boster Scientific Support
Answered: 2019-10-03
Question
What is the detection purification method for EK0842?
Verified customer
Asked: 2019-10-03
Answer
For the Human TNFSF11/RANKL ELISA Kit PicoKine™ (EK0842), the detection purification is Affinity Purified.
Boster Scientific Support
Answered: 2019-10-03
Question
What is the standard source and expression for EK0842?
Verified customer
Asked: 2019-10-03
Answer
For the Human TNFSF11/RANKL ELISA Kit PicoKine™ (EK0842), the standard source is Recombinant Protein and expression is NSO.
Boster Scientific Support
Answered: 2019-10-03
Question
What is the immunogen for the capture antibody of EK0842?
Verified customer
Asked: 2019-10-03
Answer
The immunogen for the capture antibody of the Human TNFSF11/RANKL ELISA Kit PicoKine™ (EK0842) is G64-D245.
Boster Scientific Support
Answered: 2019-10-03
Question
Can EK0842 detect free RANKL and RANKL bound to osteoprotergerin?<br>Keywords: free, bound, osteoprotergerin
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
Yes, EK0842 can detect free RANKL and RANKL bound to osteoprotergerin.
Boster Scientific Support
Answered: 2019-09-24
Question
Can EK0842 kit detect both cytoplasm and membrane bound RANKL?
Verified customer
Asked: 2019-08-22
Answer
The Human TNFSF11/RANKL ELISA Kit PicoKine EK0842 can only detect extracellular secreted protein. The secreted protein is synthesized in the intracellular space of cells.
Boster Scientific Support
Answered: 2019-08-27
Question
The customer replied with the following, please assist. "You mean this kit can't it detect cytoplasm and membrane bound RANKL? Why does it only detect extracellular secreted protein? And Why Do You Guarantee Cell Lysates? It Won't Include Secreted Protein?"
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
The secreted protein is synthesized in the intracellular space of cells. And the kit is able to detect patial intracellular secreted protein which hasn't released into extracellular space.
Boster Scientific Support
Answered: 2019-08-19
Question
For these two kits: Human TNFSF11/RANKL PicoKine
Verified Customer
Verified customer
Asked: 2019-07-30
Answer
Our lab technician suggest to use serum. Please see the following expected range for plasma and serum from healthy human.
Boster Scientific Support
Answered: 2019-07-30
Question
Would you recommend using protease inhibitor for this type of ELISA?
Verified customer
Asked: 2019-07-26
Answer
We recommend using a Protease inhibitor for the Human TNFSF11/RANKL ELISA Kit PicoKine™ (EK0842).
Boster Scientific Support
Answered: 2019-07-29
Question
Would lysing samples in deionised H2O by freeze-thaw cycle be a suitable step for EK0842?
Verified customer
Asked: 2019-07-26
Answer
Lysing samples in deionised H2O by freeze-thaw cycle is not suitable for the Human TNFSF11/RANKL ELISA Kit PicoKine™ (EK0842).
Boster Scientific Support
Answered: 2019-07-29
Question
Is there a need to centrifuge the cell lysate before perfoming the ELISA?
Verified customer
Asked: 2019-07-26
Answer
Please centrifuge the cell lysate before performing the ELISA.
Boster Scientific Support
Answered: 2019-07-29
Question
Q: can you suggest the dilution ratio of serum samples for detection of TRANCE in Human plasma? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
P. Hernandez
Verified customer
Asked: 2019-06-14
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the TRANCE ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-06-14
Question
What cells were tested with this kit and how ere the cell lysate samples prepared?
Verified Customer
Verified customer
Asked: 2019-03-20
Answer
Human peripheral blood mononuclear cells (PBMCs) lysate was tested. RIPA lysis buffer was used at 1:5 by cell volume ratio, and the dilution is 1:1.
Boster Scientific Support
Answered: 2019-03-20
Question
Q: What is the optimal O.D. value for TRANCE ELISA kit? I performed your TRANCE ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain TRANCE even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-08-18
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this TRANCE ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of TRANCE that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2018-08-18
Question
I just want to know about Human TNFSF11/RANKL ELISA Kit; there were studies that detects endogenous levels of total RANKL, so does it mean that the antibody identifies all the three isoforms either in soluble and membrane forms?
Verified Customer
Verified customer
Asked: 2018-04-19
Answer
The Human TNFSF11/RANKL ELISA Kit binds to the C-terminal extracellular topological domain of isoforms 1, 2, and 3 (based on prediction). The amino acid range of this immunogen is aa 60-110 of human TNF ligand super family member 11 with accession number O14788 (http://www.uniprot.org/uniprot/O14788).
Boster Scientific Support
Answered: 2018-04-19
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
B. Perez
Verified customer
Asked: 2018-01-08
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-01-08
Question
To Boster Technical Support: We ordered your product EK0842 Human TNFSF11/RANKL ELISA Kit for protein stimulation, however we could not see information about the concentration/dilution conditions for the treatment. Could you give the approximate amounts of RANKL to use?
Verified Customer
Verified customer
Asked: 2017-07-28
Answer
We tested the activity of EK0842 Human TNFSF11/RANKL ELISA Kit by measuring the induction of NFkappaB and 50% of its maximum conduction reached 10-25ng/ml of RANKL.
Boster Scientific Support
Answered: 2017-07-28
Question
Q: if the enzyme conjugated TRANCE antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TRANCE antigen?
Verified Customer
Verified customer
Asked: 2016-02-24
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2016-02-24
Question
What is the specificity of the RANKL antibody? (i.e. Which epitopes are recognized?)
Verified Customer
Verified customer
Asked: 2015-05-19
Answer
The standard for this antibody is recombinant human TNFSF11 (NSO, G64-D245). Therefore, the ELISA kit can detect the soluble forms derived from the cleavage of membrane-bound protein (aa. 140-317) or alternative splicing (aa. 74-317).
Boster Scientific Support
Answered: 2015-05-19
Question
Q: Can TRANCE ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
M. Chen
Verified customer
Asked: 2015-02-07
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2015-02-07
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring TRANCE using the EK0842 Human TRANCE Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2014-02-05
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2014-02-05
