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Product Info Summary
| SKU: | EK0500 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin) |
Product info
Product Name
Mouse SDF-1/CXCL12 ELISA Kit PicoKine®
SKU/Catalog Number
EK0500
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse SDF-1/CXCL12 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse CXCL12 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse SDF-1/CXCL12 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0500)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: K22-M93
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0500 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0500 is reactive to CXCL12 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin)
Application Guarantee
EK0500 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CXCL12
SDF-1 (stromal cell-derived factor-1) is small cytokine belonging to the chemokine family that is officially designated Chemokine (C-X-C motif) ligand 12 (CXCL12). This gene is located on chromosome 10q11.1. SDF-1 is produced in two forms, SDF-1alpha/CXCL12a and SDF-1beta/CXCL12b, by alternate splicing of the same gene. Chemokines are characterized by the presence of four conserved cysteines, which form two disulfide bonds. The CXCL12 proteins belong to the group of CXC chemokines, whose initial pair of cysteines are separated by one intervening amino acid. CXCL12 is strongly chemotactic for lymphocytes. CXCL12 was shown to be expressed in many tissues in mice (including brain, thymus, heart, lung, liver, kidney, spleen and bone marrow). CXCL12 is a highly efficacious lymphocyte chemoattractant. In addition, CXCL12 induces intracellular actin polymerization in lymphocytes. CXCL12 is a substrate for the matrix metalloproteinase-2, which cleaves an CXCL12 N-terminal tetrapeptide. The standard product used in this kit is recombinant SDF-1 with the molecular mass of 8Kda.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0500-CAP | Anti-Mouse CXCL12 Pre-coated 96-well strip microplate | 1 |
| EK0500-ST | Mouse CXCL12 Standard | 2 vials, 10ng/tube |
| EK0500-DA | Mouse CXCL12 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1,000 | 2,000 | 4,000 |
| O.D. | 0.039 | 0.072 | 0.119 | 0.198 | 0.431 | 0.675 | 1.352 | 2.023 |
Data Example Images
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Mouse SDF-1/CXCL12 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse SDF-1/CXCL12 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 118 | 692 | 1469 | 115 | 707 | 1562 |
| Standard deviation | 5.31 | 49.13 | 60.23 | 5.64 | 36.76 | 90.6 |
| CV (%) | 4.5% | 7.1% | 4.1% | 4.9% | 5.2% | 5.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CXCL12 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 118 | 122 | 115 | 109 | 116 | 4.18 | 3.6% |
| Sample 2 | 692 | 705 | 712 | 699 | 702 | 42.82 | 6.1% |
| Sample 3 | 1469 | 1455 | 1545 | 1547 | 1504 | 82.72 | 5.5% |
Protein Target Info & Infographic
Gene/Protein Information For CXCL12 (Source: Uniprot.Org, NCBI)
Gene Name
CXCL12
Full Name
Stromal cell-derived factor 1
Weight
13634 MW
Superfamily
intercrine alpha (chemokine CxC) family
Alternative Names
Stromal cell-derived factor 1 ;Cxcl12 ; CXCL12 IRH, PBSF, SCYB12, SDF1, TLSF, TPAR1 C-X-C motif chemokine ligand 12 stromal cell-derived factor 1|chemokine (C-X-C motif) ligand 12|intercrine reduced in hepatomas|pre-B cell growth-stimulating factor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CXCL12, check out the CXCL12 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CXCL12: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse SDF-1/CXCL12 ELISA Kit PicoKine® (EK0500)
Hello CJ!
EK0500 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhang H, Teng X, Liu Z, Zhang L, Liu Z. J Exp Clin Cancer Res. 2015 Feb 12;34:16. Doi: 10.1186/S13046-015-0132-Y. Gene Expression Profile Analyze The Molecular Mechanism Of Cxcr7 Regulating Papillary Thyroid Carcinoma Growth And Metastasis.
Lin L, Han Mm, Wang F, Xu Ll, Yu Hx, Yang Py. Cell Death Dis. 2014 Oct 23;5:E1488. Doi: 10.1038/Cddis.2014.392. Cxcr7 Stimulates Mapk Signaling To Regulate Hepatocellular Carcinoma Progression.
Wu S, Li L, Wang G, Shen W, Xu Y, Liu Z, Zhuo Z, Xia H, Gao Y, Tan K. Int J Nanomedicine. 2014 Dec 3;9:5639-51. Doi: 10.2147/Ijn.S73950. Ecollection 2014. Ultrasound-Targeted Stromal Cell-Derived Factor-1-Loaded Microbubble Destruction Promotes Me...
Tong J, Ding J, Shen X, Chen L, Bian Y, Ma G, Yao Y, Yang F. Plos One. 2013 Nov 14;8(11):E80186. Doi: 10.1371/Journal.Pone.0080186. Ecollection 2013. Mesenchymal Stem Cell Transplantation Enhancement In Myocardial Infarction Rat Model Under Ultras...
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Customer Reviews
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Customer Q&As
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9 Customer Q&As for Mouse SDF-1/CXCL12 ELISA Kit PicoKine®
Question
Q: if the enzyme conjugated CXCL12 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CXCL12 antigen?
Verified Customer
Verified customer
Asked: 2020-07-10
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-07-10
Question
Q: Can CXCL12 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
B. Banerjee
Verified customer
Asked: 2020-06-23
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-06-23
Question
Q: can I use EDTA plasma as samples in Mouse CXCL12 Picokine® ELISA Kit (Catalog # EK0500)?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of CXCL12 causing degradation of its protein structure. CXCL12 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the CXCL12 ELISA, treating samples with different anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-11-19
Question
Q: What is the optimal O.D. value for CXCL12 ELISA kit? I performed your CXCL12 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain CXCL12 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2019-11-02
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CXCL12 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of CXCL12 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2019-11-02
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of CXCL12 in Mouse serum? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2019-04-20
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CXCL12 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-04-20
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis CXCL12 using the EK0500 Mouse CXCL12 Picokine® ELISA Kit?
M. Lee
Verified customer
Asked: 2018-09-21
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2018-09-21
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-09-10
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-09-10
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-07-12
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-07-12
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
J. Parker
Verified customer
Asked: 2017-06-30
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2017-06-30
