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Product Info Summary
| SKU: | EK1190 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) and urine. |
Product info
Product Name
Mouse SAA/SAA1 ELISA Kit PicoKine®
SKU/Catalog Number
EK1190
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse SAA/SAA1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Saa1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse SAA/SAA1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1190)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: G20-Y122
Sensitivity
<20 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1190 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1190 is reactive to Saa1 in Mouse samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
Application Guarantee
EK1190 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of SAA1
Serum amyloid A protein (SAA), also known as SAA1, is a protein that in humans is encoded by the SAA1 gene. This gene encodes a member of the serum amyloid A family of apolipoproteins. It is mapped to 11p15.1. SAA is a major acute phase protein that is highly expressed in response to inflammation and tissue injury. This protein also plays an important role in HDL metabolism and cholesterol homeostasis. High levels of this protein are associated with chronic inflammatory diseases including atherosclerosis, rheumatoid arthritis, Alzheimer's disease and Crohn's disease. This protein may also be a potential biomarker for certain tumors.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1190-CAP | Anti-Mouse Saa1 Pre-coated 96-well strip microplate | 1 |
| EK1190-ST | Mouse Saa1 Standard | 2 vials, 100 ng/tube |
| EK1190-DA | Mouse Saa1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.079 | 0.108 | 0.215 | 0.437 | 0.535 | 0.990 | 1.423 | 1.872 |
Data Example Images
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Mouse SAA PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect SAA1, Dilution ratio of 1:5, concentration in serum and plasma is 90-125 ng/ml..
Some articles we found to cite concentrations of SAA1 in samples: 24520213 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse SAA/SAA1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 270 | 1129 | 2924 | 194 | 1044 | 3393 |
| Standard deviation | 9.26 | 70.9 | 166.67 | 9.72 | 52.93 | 245.81 |
| CV (%) | 3.4% | 6.3% | 5.7% | 5% | 5.1% | 7.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of SAA1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 270 | 207 | 211 | 200 | 222 | 16.23 | 7.3% |
| Sample 2 | 1129 | 1020 | 856 | 655 | 915 | 32.12 | 3.5% |
| Sample 3 | 2924 | 4991 | 2925 | 4112 | 3738 | 240.73 | 6.4% |
Protein Target Info & Infographic
Gene/Protein Information For Saa1 (Source: Uniprot.Org, NCBI)
Gene Name
Saa1
Full Name
Serum amyloid A-1 protein
Weight
13770 MW
Superfamily
SAA family
Alternative Names
Serum amyloid A-1 protein;Saa1; SAA1 PIG4, SAA, SAA2, TP53I4 serum amyloid A1 serum amyloid A-1 protein|serum amyloid A protein|tumor protein p53 inducible protein 4
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Saa1, check out the Saa1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Saa1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse SAA/SAA1 ELISA Kit PicoKine® (EK1190)
Hello CJ!
EK1190 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse
Fluid supplementation accelerates epithelial repair during chemical colitis
Therapeutic effects of rosuvastatin in hypercholesterolemic prediabetic mice in the absence of low density lipoprotein receptor
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Customer Reviews
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Customer Q&As
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18 Customer Q&As for Mouse SAA/SAA1 ELISA Kit PicoKine®
Question
What is the capture host/clonality for EK1190?
Verified customer
Asked: 2020-11-12
Answer
For the Mouse SAA/SAA1 ELISA Kit PicoKine (EK1190), the capture host/clonality is Goat Polyclonal.
Boster Scientific Support
Answered: 2020-11-13
Question
What is the detection host/clonality for EK1190?
Verified customer
Asked: 2020-11-12
Answer
For the Mouse SAA/SAA1 ELISA Kit PicoKine (EK1190), the detection host/clonality is Goat Polyclonal.
Boster Scientific Support
Answered: 2020-11-13
Question
What is the capture purification for EK1190?
Verified customer
Asked: 2020-11-12
Answer
For the Mouse SAA/SAA1 ELISA Kit PicoKine (EK1190), the capture purification is Affinitty Purified.
Boster Scientific Support
Answered: 2020-11-13
Question
What is the detection purification for EK1190?
Verified customer
Asked: 2020-11-12
Answer
For the Mouse SAA/SAA1 ELISA Kit PicoKine (EK1190), the detection purification is Affinitty Purified.
Boster Scientific Support
Answered: 2020-11-13
Question
What is the standard source for EK1190?
Verified customer
Asked: 2020-11-12
Answer
For the Mouse SAA/SAA1 ELISA Kit PicoKine (EK1190), the standard source is Recombinant Protein.
Boster Scientific Support
Answered: 2020-11-13
Question
What is the standard expression for EK1190?
Verified customer
Asked: 2020-11-12
Answer
For the Mouse SAA/SAA1 ELISA Kit PicoKine (EK1190), the standard expression is E. coli Expression System.
Boster Scientific Support
Answered: 2020-11-13
Question
Does EK1190 contain any baculovirus or any related virus materials?
Verified customer
Asked: 2020-11-12
Answer
The Mouse SAA/SAA1 ELISA Kit PicoKine (EK1190) doesn't contain any baculovirus or any related virus materials.
Boster Scientific Support
Answered: 2020-11-13
Question
What are the tested samples for EK1190?
Verified customer
Asked: 2020-11-12
Answer
For the Mouse SAA/SAA1 ELISA Kit PicoKine (EK1190), the tested samples are Mouse cell culture supernates, serum, plasma(heparin, EDTA) and urine.
Boster Scientific Support
Answered: 2020-11-13
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-09-16
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-09-16
Question
Q: Can SAA1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-11-20
Question
Does EK1190 recognize only SAA1, or can it also recognize SAA2 and SAA3? Keywords: target protein, specificity, isoforms
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
EK1190 recognizes SAA2 as well, but no significant cross-reactivity was observed with mouse SAA3.
Boster Scientific Support
Answered: 2019-06-20
Question
Q: can I use citrate plasma as samples in Mouse SAA1 Picokine® ELISA Kit (Catalog # EK1190)?
L. Thompson
Verified customer
Asked: 2018-12-06
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of SAA1 causing disruption of its protein structure. SAA1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the SAA1 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-12-06
Question
Q: What is the optimal O.D. value for SAA1 ELISA kit? I performed your SAA1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain SAA1 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-11-22
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this SAA1 ELISA kit to have sensitivity of 150pg/ml, that means the minimum amount of SAA1 that can be declared/interpreted as positive by the above standard is 150pg/ml.
Boster Scientific Support
Answered: 2018-11-22
Question
Q: can you suggest the dilution ratio of serum samples for detection of SAA1 in Mouse serum? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-09-17
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the SAA1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-09-17
Question
Will this ELISA kit have cross reactions with SAA2 and SAA3? Keywords: cross reactivity, specificity, target protein
Verified Customer
Verified customer
Asked: 2017-07-21
Answer
EK1190 has high cross reaction with mouse SAA2 protein, and has a little cross reaction with mouse SAA3 protein.
Boster Scientific Support
Answered: 2017-07-21
Question
Q: if the enzyme conjugated SAA1 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the SAA1 antigen?
G. Wright
Verified customer
Asked: 2016-10-06
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2016-10-06
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2015-12-02
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2015-12-02
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring SAA1 using the EK1190 Mouse SAA1 Picokine® ELISA Kit?
W. Johnson
Verified customer
Asked: 2015-11-26
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2015-11-26
