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Product Info Summary
| SKU: | EK1544 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human SAA/SAA1 ELISA Kit PicoKine®
SKU/Catalog Number
EK1544
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human SAA/SAA1 ELISA Kit PicoKine® (96 Tests). Quantitate Human SAA1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 150pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human SAA/SAA1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1544)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: S20-Y122
Sensitivity
<150 pg/ml
Assay Range
1.56 ng/ml - 100 ng/ml
Standard Dilution Instructions
See datasheet of EK1544 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1544 is reactive to SAA1 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1544 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of SAA1
Serum amyloid A protein (SAA), also known as SAA1, is a protein that in humans is encoded by the SAA1 gene. This gene encodes a member of the serum amyloid A family of apolipoproteins. It is mapped to 11p15.1. SAA is a major acute phase protein that is highly expressed in response to inflammation and tissue injury. This protein also plays an important role in HDL metabolism and cholesterol homeostasis. High levels of this protein are associated with chronic inflammatory diseases including atherosclerosis, rheumatoid arthritis, Alzheimer's disease and Crohn's disease. This protein may also be a potential biomarker for certain tumors.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1544-CAP | Anti-Human SAA1 Pre-coated 96-well strip microplate | 1 |
| EK1544-ST | Human SAA1 Standard | 2 vials, 100 ng/tube |
| EK1544-DA | Human SAA1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (ng/ml) | 0 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 |
| O.D. | 0.001 | 0.085 | 0.167 | 0.356 | 0.711 | 1.083 | 1.773 | 2.149 |
Data Example Images
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Human SAA/SAA1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect SAA1, For serum, dilution ratio is 1:20 and concentration is around 140-287 ng/ml.
For plasma, dilution ratio is 1:1 and concentration is around 20 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human SAA/SAA1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 3858 | 13218 | 48138 | 4235 | 13787 | 47081 |
| Standard deviation | 293.2 | 581.59 | 2406.9 | 393.85 | 868.58 | 2777.77 |
| CV (%) | 7.6% | 4.4% | 5% | 9.3% | 6.3% | 5.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of SAA1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 3858 | 3499 | 3839 | 4125 | 3830 | 222.17 | 5.8% |
| Sample 2 | 13218 | 13736 | 13026 | 14070 | 13512 | 413.57 | 3% |
| Sample 3 | 48138 | 46567 | 52708 | 53611 | 50256 | 2973.33 | 5.9% |
Protein Target Info & Infographic
Gene/Protein Information For SAA1 (Source: Uniprot.Org, NCBI)
Gene Name
SAA1
Full Name
Serum amyloid A-1 protein
Weight
13532 MW
Superfamily
SAA family
Alternative Names
Serum amyloid A-1 protein;SAA;Amyloid protein A;Amyloid fibril protein AA;Serum amyloid protein A (2-104);Serum amyloid protein A (3-104);Serum amyloid protein A (2-103);Serum amyloid protein A (2-102);Serum amyloid protein A (4-101);SAA1; SAA1 PIG4, SAA, SAA2, TP53I4 serum amyloid A1 serum amyloid A-1 protein|serum amyloid A protein|tumor protein p53 inducible protein 4
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on SAA1, check out the SAA1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SAA1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human SAA/SAA1 ELISA Kit PicoKine® (EK1544)
Hello CJ!
EK1544 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse
Serum amyloid a protein as a potential biomarker in predicting acute onset and association with in-hospital death in acute aortic dissection
Recommended Resources
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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9 Customer Q&As for Human SAA/SAA1 ELISA Kit PicoKine®
Question
Does EK1544 Kit detect the C-Terminus? Which of the SAA (1,2,4 or even all) is detected?
Verified customer
Asked: 2020-06-10
Answer
Our lab hasn't performed epitope mapping using the Human SAA/SAA1 ELISA Kit PicoKine™ EK1544. It might be able to detect the C-Terminus. SAA1 is detected. Less than 5% cross reactivity with SAA2 was observed. Cross reactivity with SAA4 hasn't been tested.
Boster Scientific Support
Answered: 2020-06-12
Question
Q: how can I thaw whole blood sample for SAA1 ELISA after freezing?
P. Lopez
Verified customer
Asked: 2020-02-11
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test SAA1 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-02-11
Question
Q: Can SAA1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
T. Mangal
Verified customer
Asked: 2019-05-10
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-05-10
Question
Q: how do I analyze ELISA data? I measured SAA1 level in plasma.
Verified Customer
Verified customer
Asked: 2018-10-28
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-10-28
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis SAA1 using the EK1544 Human SAA1 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-05-17
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2018-05-17
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2017-12-09
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2017-12-09
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2017-03-10
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2017-03-10
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2016-04-23
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2016-04-23
Question
Q: can you recommend the dilution ratio of serum samples for detection of SAA1 in Human plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Y. Singh
Verified customer
Asked: 2016-02-16
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the SAA1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2016-02-16
