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Product Info Summary
| SKU: | FEK0862 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (EDTA). |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Mouse PTX3/Pentraxin 3 PicoKine® Quick ELISA Kit
See all Pentraxin 3/TSG-14 products
SKU/Catalog Number
FEK0862
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse PTX3/Pentraxin 3 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Ptx3 in cell culture supernatants, serum and plasma (EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Mouse PTX3/Pentraxin 3 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0862)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: E18-S381
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK0862 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0862 is reactive to ptx3 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (EDTA).
Application Guarantee
FEK0862 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Pentraxin 3/TSG-14
PTX3 (Pentraxin 3) is a member of the pentraxin superfamily. This super family characterized by cyclic multimeric structure. The PTX3 gene is mapped to 3q25.32. The predicted 381-amino acid PTX3 protein has homology to the pentraxin protein family. Significant levels of PTX3 were detected in plasma of neutropenic patients with systemic A. PTX3 is effective in preventing CMV infection and reactivation, as well as subsequent Aspergillus infection. PTX3 activates the classical pathway of complementactivation and facilitates pathogen recognition by macrophages and DCs.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK0862-CAP | Anti-Mouse Pentraxin 3/TSG-14 Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK0862-ST | Mouse Pentraxin 3/TSG-14 Standard | 2 | 10 ng/tube |
| FEK0862-H | HRP Linked Anti-Mouse ptx3 Antibody | 1 | 6ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.035 | 0.119 | 0.209 | 0.344 | 0.626 | 1.028 | 1.542 | 2.207 |
Data Example Images
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Mouse PTX3/Pentraxin 3 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse PTX3/Pentraxin 3 PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 262 | 1334 | 4146 | 264 | 1451 | 3917 |
| Standard deviation | 15.19 | 98.71 | 211.44 | 15.84 | 113.17 | 274.19 |
| CV (%) | 5.8% | 7.4% | 7% | 6% | 7.8% | 7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Pentraxin 3/TSG-14 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 262 | 259 | 249 | 272 | 260 | 8.2 | 3.1% |
| Sample 2 | 1334 | 1350 | 1444 | 1511 | 1409 | 71.99 | 5.1% |
| Sample 3 | 4146 | 4596 | 4000 | 4523 | 4316 | 250 | 5.7% |
Protein Target Info & Infographic
Gene/Protein Information For ptx3 (Source: Uniprot.Org, NCBI)
Gene Name
ptx3
Full Name
Pentraxin-related protein PTX3
Weight
41.976kDa
Alternative Names
alpha-induced protein 5; pentaxin-related gene, rapidly induced by IL-1 beta, tumor necrosis factor; Pentaxin-related protein PTX3; Pentraxin 3; pentraxin 3, long; pentraxin-3; pentraxin-related gene, rapidly induced by IL-1 beta; pentraxin-related protein PTX3; PTX3; TNF alpha-induced protein 5; TNFAIP5; TSG14; TSG-14; TSG-14pentaxin-related gene, rapidly induced by IL-1 beta; Tumor necrosis factor alpha-induced protein 5; tumor necrosis factor, alpha-induced protein 5; Tumor necrosis factor-inducible gene 14 protein; tumor necrosis factor-inducible protein TSG-14 PTX3 TNFAIP5, TSG-14 pentraxin 3 pentraxin-related protein PTX3|TNF alpha-induced protein 5|long pentraxin 3|tumor necrosis factor alpha-induced protein 5|tumor necrosis factor-inducible gene 14 protein|tumor necrosis factor-inducible protein TSG-14
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on ptx3, check out the ptx3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ptx3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse PTX3/Pentraxin 3 PicoKine® Quick ELISA Kit (FEK0862)
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