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Product Info Summary
| SKU: | EK0861 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (EDTA) and saliva. |
Product info
Product Name
Human PTX3/Pentraxin 3 ELISA Kit PicoKine®
View all Pentraxin 3/TSG-14 ELISA kits
SKU/Catalog Number
EK0861
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human PTX3/Pentraxin 3 ELISA Kit PicoKine® (96 Tests). Quantitate Human PTX3 in cell culture supernatants, serum, plasma (EDTA) and saliva. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human PTX3/Pentraxin 3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0861)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: E18-S381
Sensitivity
<10 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0861 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0861 is reactive to PTX3 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (EDTA) and saliva.
Application Guarantee
EK0861 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Pentraxin 3/TSG-14
PTX3 (Pentraxin 3) is a member of the pentraxin superfamily. This super family characterized by cyclic multimeric structure. The PTX3 gene is mapped to 3q25.32. The predicted 381-amino acid PTX3 protein has homology to the pentraxin protein family. Significant levels of PTX3 were detected in plasma of neutropenic patients with systemic A. PTX3 is effective in preventing CMV infection and reactivation, as well as subsequent Aspergillus infection. PTX3 activates the classical pathway of complementactivation and facilitates pathogen recognition by macrophages and DCs.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0861-CAP | Anti-Human PTX3 Pre-coated 96-well strip microplate | 1 |
| EK0861-ST | Human PTX3 Standard | 2 vials, 20 ng/tube |
| EK0861-DA | Human PTX3 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
| O.D. | 0.052 | 0.150 | 0.278 | 0.420 | 0.649 | 1.111 | 1.605 | 1.941 |
Data Example Images
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Human PTX3/Pentraxin 3 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Pentraxin 3/TSG-14, Dilution ratio of 1:1, concentration in serum and plasma is around 350 pg/ml..
Some articles we found to cite concentrations of Pentraxin 3/TSG-14 in samples: 25435748 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human PTX3/Pentraxin 3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 662 | 1701 | 9194 | 718 | 1816 | 9601 |
| Standard deviation | 33.76 | 85.05 | 579.22 | 38.77 | 92.61 | 662.46 |
| CV (%) | 6.9% | 5% | 6.9% | 5.4% | 6.9% | 6.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Pentraxin 3/TSG-14 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 662 | 620 | 693 | 604 | 644 | 34.99 | 5.4% |
| Sample 2 | 1701 | 1556 | 1536 | 1612 | 1601 | 63.97 | 3.9% |
| Sample 3 | 9194 | 8999 | 8940 | 8763 | 8974 | 153.86 | 1.7% |
Protein Target Info & Infographic
Gene/Protein Information For PTX3 (Source: Uniprot.Org, NCBI)
Gene Name
PTX3
Full Name
Pentraxin-related protein PTX3
Weight
41976 MW
Alternative Names
Pentraxin-related protein PTX3;Pentaxin-related protein PTX3;Tumor necrosis factor alpha-induced protein 5;TNF alpha-induced protein 5;Tumor necrosis factor-inducible gene 14 protein;TSG-14;PTX3;TNFAIP5, TSG14; PTX3 TNFAIP5, TSG-14 pentraxin 3 pentraxin-related protein PTX3|TNF alpha-induced protein 5|long pentraxin 3|tumor necrosis factor alpha-induced protein 5|tumor necrosis factor-inducible gene 14 protein|tumor necrosis factor-inducible protein TSG-14
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on PTX3, check out the PTX3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PTX3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human PTX3/Pentraxin 3 ELISA Kit PicoKine® (EK0861)
Hello CJ!
EK0861 has been cited in 28 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
A new scoring system for early diagnosis of ventilator-associated pneumonia: LUPPIS
Pentraxin 3 as a New Cardiovascular Marker in Adrenal Adenomas
Tunicamycin enhances the suppressive effects of cisplatin on lung cancer growth through PTX3 glycosylation via AKT/NF-κB signaling pathway
Scorpion Venom Analgesic Peptide, BmK AGAP Inhibits Stemness, and Epithelial-Mesenchymal Transition by Down-Regulating PTX3 in Breast Cancer
Changes of serum pentraxin-3 and hypersensitive CRP levels during pregnancy and their relationship with gestational diabetes mellitus
Evaluating pentraxin-3 and hypersensitivity CRP expression in obese pregnancies
Clinical study of Presepsin and Pentraxin3 in critically ill children
The relationship of pentraxin-3 levels with IL-17, fetuin-A, insulin in patients with Behçets disease
Effect of combined exercise training on pentraxins and pro- inflammatory cytokines in people with multiple sclerosis as a function of disability status
Levels of serum pentraxin 3, IL-6, fetuin A and insulin in patients with rheumatoid arthritis
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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11 Customer Q&As for Human PTX3/Pentraxin 3 ELISA Kit PicoKine®
Question
Can MNB4 antibody be suitable in the measurement of the PTX3?
Verified customer
Asked: 2020-12-09
Answer
Unfortunatley, our lab hasn't used antibody MNB4 in the measurement of the PTX3 and we are uncertian if could be used.
Boster Scientific Support
Answered: 2020-12-09
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
M. Green
Verified customer
Asked: 2020-06-24
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-06-24
Question
Can the Human PTX3/Pentraxin 3 PicoKine™ ELISA Kit be used on heparinized plasma?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
The Human PTX3/Pentraxin 3 PicoKine™ ELISA Kit has been validated with plasma samples using EDTA as a coagulant. Serum and heparin plasma are not suitable for use in this assay.
Boster Scientific Support
Answered: 2019-09-24
Question
Q: is it okay to use edta plasma as samples in Human Pentraxin 3 Picokine® ELISA Kit (Catalog # EK0861)?
R. Zhou
Verified customer
Asked: 2019-09-12
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of Pentraxin 3 causing degradation of its protein structure. Pentraxin 3 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Pentraxin 3 ELISA, treating samples with various anticoagulants and decided that EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-09-12
Question
Q: Can Pentraxin 3 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
T. Singh
Verified customer
Asked: 2019-02-19
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-02-19
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2018-12-28
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-12-28
Question
Q: how to proceed with the analysis of ELISA data? I have obtained Pentraxin 3 level in plasma.
Verified Customer
Verified customer
Asked: 2018-06-24
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-06-24
Question
Q: What is the optimal O.D. value for Pentraxin 3 ELISA kit? I performed your Pentraxin 3 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Pentraxin 3 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-06-10
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Pentraxin 3 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Pentraxin 3 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2018-06-10
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-06-10
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-06-10
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of Pentraxin 3 in Human plasma? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2017-12-30
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Pentraxin 3 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2017-12-30
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Pentraxin 3 using the EK0861 Human Pentraxin 3 Picokine® ELISA Kit?
R. Zhou
Verified customer
Asked: 2017-11-27
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2017-11-27
