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Product Info Summary
| SKU: | EK0433 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Mouse IL-18 ELISA Kit PicoKine®
View all IL-18/IL-1F4 ELISA kits
SKU/Catalog Number
EK0433
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse IL-18 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il18 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse IL-18 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0433)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: N36-S192
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0433 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0433 is reactive to IL18 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK0433 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL-18/IL-1F4
Interleukin(IL)-18, also called Interferon-gamma-inducing factor(IGIF), augments natural killer(NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids.1 IL-18 is a recently discovered cytokine that modulates both T helper type 1(Th1) and Th2 responses.2 IL-18 is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.3
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0433-CAP | Anti-Mouse IL18 Pre-coated 96-well strip microplate | 1 |
| EK0433-ST | Mouse IL18 Standard | 2 vials, 10 ng/tube |
| EK0433-DA | Mouse IL18 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 | 2,000 |
| O.D. | 0.041 | 0.088 | 0.174 | 0.266 | 0.433 | 0.809 | 1.322 | 1.976 |
Data Example Images
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Mouse IL-18 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IL-18/IL-1F4, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 31.2 pg/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse IL-18 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 51 | 286 | 905 | 59 | 310 | 933 |
| Standard deviation | 2.14 | 11.15 | 41.63 | 3.07 | 17.05 | 45.72 |
| CV (%) | 4.2% | 3.9% | 4.6% | 5.2% | 5.5% | 4.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IL-18/IL-1F4 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 51 | 54 | 57 | 60 | 56 | 1.44 | 2.6% |
| Sample 2 | 286 | 302 | 292 | 297 | 294 | 9.12 | 3.1% |
| Sample 3 | 905 | 880 | 869 | 892 | 887 | 24.42 | 2.8% |
Protein Target Info & Infographic
Gene/Protein Information For IL18 (Source: Uniprot.Org, NCBI)
Gene Name
IL18
Full Name
Interleukin-18
Weight
Superfamily
IL-1 family
Alternative Names
Iboctadekin; IFN-gamma-inducing factor; IGIF; IGIFIL-1 gamma; IL18; IL-18; IL-18MGC12320; IL-1F4; IL1F4iboctadekin; IL-1g; Interferon gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; interleukin-18 IL18 IGIF, IL-18, IL-1g, IL1F4 interleukin 18 interleukin-18|IFN-gamma-inducing factor|IL-1 gamma|iboctadekin|interleukin 18 (interferon-gamma-inducing factor)|interleukin-1 gamma
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IL18, check out the IL18 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IL18: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse IL-18 ELISA Kit PicoKine® (EK0433)
Hello CJ!
EK0433 has been cited in 9 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Protective Effect of Oxytocin on Ventilator-Induced Lung Injury Through NLRP3-Mediated Pathways
LncRNA MALAT1 facilitates inflammasome activation via epigenetic suppression of Nrf2 in Parkinson’s disease
Protein kinase C is involved in the neuroprotective effect of berberine against intrastriatal injection of quinolinic acid‐induced biochemical alteration in mice
Anti-Alzheimers disease activity of secondary metabolites from Xanthoceras sorbifolia Bunge
Flavonoid VI-16 protects against DSS-induced colitis by inhibiting Txnip-dependent NLRP3 inflammasome activation in macrophages via reducing oxidative stress
Involvement of the microglial NLRP3 inflammasome in the anti-inflammatory effect of the antidepressant clomipramine
Piperine ameliorated lupus nephritis by targeting AMPK-mediated activation of NLRP3 inflammasome
Polysaccharide from Scutellaria baicalensis Georgi ameliorates colitis via suppressing NF-κB signaling and NLRP3 inflammasome activation
Knockdown of Long Noncoding RNAs of Maternally Expressed 3 Alleviates Hyperoxia-Induced Lung Injury via Inhibiting Thioredoxin-Interacting Protein–Mediated Pyroptosis by Binding to miR-18a
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