Rat IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine®

IL-18/IL-1F4 ELISA kit for Rat

Rat IL-18 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Il18 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml. Cited in 10 publication(s).

Product Info Summary

SKU: EK0592
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Rat IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine®

View all IL-18/IL-1F4 ELISA kits

SKU/Catalog Number

EK0592

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat IL-18 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Il18 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Rat IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0592)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: H37-S194

Sensitivity

<1 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0592 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0592 is reactive to IL18 in Rat samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0592 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of IL-18/IL-1F4

Interleukin (IL)-18, also called Interferon-gamma-inducing factor (IGIF), augments natural killer (NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids.1 IL-18 is a recently discovered cytokine that modulates both T helper type 1 (Th1) and Th2 responses.2 IL-18 is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.3

Kit Components

Catalog Number Description Quantity
EK0592-CAP Anti-Rat IL18 Pre-coated 96-well strip microplate 1
EK0592-ST Rat IL18 Standard 2 vials, 10 ng/tube
EK0592-DA Rat IL18 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0270.0690.1010.1730.3230.5771.0921.856

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect IL-18/IL-1F4, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 15.6 pg/ml..

Some articles we found to cite concentrations of IL-18/IL-1F4 in samples: 23983360 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2916651129165490
Standard deviation1.972.222.992.29.0729.4
CV (%)6.8%4.7%4.5%7.6%5.5%6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IL-18/IL-1F4 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 129293028290.72.4%
Sample 21661491651521587.584.7%
Sample 351148952049950411.752.3%
*number of samples for each test n=16.

Gene/Protein Information For IL18 (Source: Uniprot.Org, NCBI)

Gene Name

IL18

Full Name

Interleukin-18

Weight

22.326kDa

Superfamily

IL-1 family

Alternative Names

Iboctadekin; IFN-gamma-inducing factor; IGIF; IGIFIL-1 gamma; IL18; IL-18; IL-18MGC12320; IL-1F4; IL1F4iboctadekin; IL-1g; Interferon gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; interleukin-18 IL18 IGIF, IL-18, IL-1g, IL1F4 interleukin 18 interleukin-18|IFN-gamma-inducing factor|IL-1 gamma|iboctadekin|interleukin 18 (interferon-gamma-inducing factor)|interleukin-1 gamma

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IL18, check out the IL18 Infographic

IL18 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IL18: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

EK0592 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Rapamycin‑induced autophagy attenuates hormone‑imbalance‑induced chronic non‑bacterial prostatitis in rats via the inhibition of NLRP3 inflammasome‑mediated inflammation

Exogenous α-calcitonin gene-related peptide attenuates lipopolysaccharide-induced acute lung injury in rats

Parkin-Dependent Mitophagy Is Required for the Inhibition of ATF4 on NLRP3 Inflammasome Activation in Cerebral Ischemia-Reperfusion Injury in Rats

Berberine Ameliorates Spatial Learning Memory Impairment and Modulates Cholinergic Anti-Inflammatory Pathway in Diabetic Rats

Berberine Ameliorates Diabetes-Associated Cognitive Decline through Modulation of Aberrant Inflammation Response and Insulin Signaling Pathway in DM Rats

Melatonin inhibits the inflammation and apoptosis in rats with diabetic retinopathy via MAPK pathway.

Exposure to nickel oxide nanoparticles induces pulmonary inflammation through NLRP3 inflammasome activation in rats

Theaflavin alleviates inflammatory response and brain injury induced by cerebral hemorrhage via inhibiting the nuclear transcription factor kappa β-related pathway in rats

The activation of IL-17 signaling pathway promotes pyroptosis in pneumonia-induced sepsis

P2X7 Receptor (P2X7R) of Microglia Mediates Neuroinflammation by Regulating (NOD)-Like Receptor Protein 3 (NLRP3) Inflammasome-Dependent Inflammation After Spinal Cord Injury

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Find answers in Q&As, reviews.

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11 Customer Q&As for Rat IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine®

Question

Is the detection antibody in EK0592 available apart from the kit, unconjugated? Are you able to reveal the clone ID, assuming it is monoclonal?

Verified customer

Asked: 2022-09-06

Answer

I am sorry to inform you that we don't sell detection antibody in EK0592 independently. Both the capture and detection antibodies in EK0592 are goat polyclonal antibodies.

Boster Scientific Support

Answered: 2022-09-07

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

W. Wilson

Verified customer

Asked: 2020-11-17

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-11-17

Question

Q: is it okay to use citrate plasma as samples in Rat IL-18 Picokine® ELISA Kit (Catalog # EK0592)?

Verified Customer

Verified customer

Asked: 2020-09-22

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of IL-18 causing disruption of its protein structure. IL-18 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the IL-18 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-09-22

Question

Has the serum / plasma MRD (Minimal Required Dilution) been calculated for EK0592 kit?

Verified customer

Asked: 2020-04-05

Answer

The serum / plasma MRD (Minimal Required Dilution) for Rat IL-18 ELISA Kit PicoKine EK0592 is 1:1.

Boster Scientific Support

Answered: 2020-04-07

Question

Q: can you suggest the dilution ratio of serum samples for detection of IL-18 in Rat serum? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.

V. Mitchell

Verified customer

Asked: 2019-08-24

Answer

A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL-18 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2019-08-24

Question

Was baculovirus expression system used for the manufacturing process of EK0592? How about recombinant Rat IL-18 standard and antibodies? Is this a recombinant protein?

Verified customer

Asked: 2019-05-15

Answer

For the Rat IL-18 ELISA Kit PicoKine™ (EK0592), E.coli expression system is used and it is a recombinant protein.

Boster Scientific Support

Answered: 2019-05-16

Question

Has EK0592 been validated on urine samples?

Verified customer

Asked: 2019-05-15

Answer

We have not tested nor validated the Rat IL-18 ELISA Kit PicoKine™ (EK0592) with urine sample. In theory the ELISA kit will work for all sample types if the target protein is present at a level that falls within the linear range of the ELISA kit detection range. For sample types that we have not tested for, we suggest to run pilot experiments and decide the optimal sample dilution.

Boster Scientific Support

Answered: 2019-05-16

Question

Q: Can IL-18 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2017-06-15

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2017-06-15

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

H. Williams

Verified customer

Asked: 2017-04-16

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2017-04-16

Question

Q: if the enzyme conjugated IL-18 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-18 antigen?

Verified Customer

Verified customer

Asked: 2016-03-19

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2016-03-19

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification IL-18 using the EK0592 Rat IL-18 Picokine® ELISA Kit?

D. Davis

Verified customer

Asked: 2014-01-28

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2014-01-28

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