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Product Info Summary
| SKU: | EK0592 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Rat IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine®
View all IL-18/IL-1F4 ELISA kits
SKU/Catalog Number
EK0592
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat IL-18 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Il18 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0592)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: H37-S194
Sensitivity
<1 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0592 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0592 is reactive to Il18 in Rat samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0592 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL-18/IL-1F4
Interleukin (IL)-18, also called Interferon-gamma-inducing factor (IGIF), augments natural killer (NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids.1 IL-18 is a recently discovered cytokine that modulates both T helper type 1 (Th1) and Th2 responses.2 IL-18 is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.3
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0592-CAP | Anti-Rat Il18 Pre-coated 96-well strip microplate | 1 |
| EK0592-ST | Rat Il18 Standard | 2 vials, 10 ng/tube |
| EK0592-DA | Rat Il18 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.027 | 0.069 | 0.101 | 0.173 | 0.323 | 0.577 | 1.092 | 1.856 |
Data Example Images
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Rat IL-18 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IL-18/IL-1F4, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 15.6 pg/ml..
Some articles we found to cite concentrations of IL-18/IL-1F4 in samples: 23983360 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 29 | 166 | 511 | 29 | 165 | 490 |
| Standard deviation | 1.97 | 2.2 | 22.99 | 2.2 | 9.07 | 29.4 |
| CV (%) | 6.8% | 4.7% | 4.5% | 7.6% | 5.5% | 6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IL-18/IL-1F4 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 29 | 29 | 30 | 28 | 29 | 0.7 | 2.4% |
| Sample 2 | 166 | 149 | 165 | 152 | 158 | 7.58 | 4.7% |
| Sample 3 | 511 | 489 | 520 | 499 | 504 | 11.75 | 2.3% |
Protein Target Info & Infographic
Gene/Protein Information For Il18 (Source: Uniprot.Org, NCBI)
Gene Name
Il18
Full Name
Interleukin-18
Weight
22303 MW
Superfamily
IL-1 family
Alternative Names
Interleukin-18;IL-18;Interferon gamma-inducing factor;IFN-gamma-inducing factor;Interleukin-1 gamma;IL-1 gamma;Il18;Igif; IL18 IGIF, IL-18, IL-1g, IL1F4 interleukin 18 interleukin-18|IFN-gamma-inducing factor|IL-1 gamma|iboctadekin|interleukin 18 (interferon-gamma-inducing factor)|interleukin-1 gamma
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Il18, check out the Il18 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Il18: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine® (EK0592)
Hello CJ!
EK0592 has been cited in 68 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Rapamycin‑induced autophagy attenuates hormone‑imbalance‑induced chronic non‑bacterial prostatitis in rats via the inhibition of NLRP3 inflammasome‑mediated inflammation
Exogenous α-calcitonin gene-related peptide attenuates lipopolysaccharide-induced acute lung injury in rats
Parkin-Dependent Mitophagy Is Required for the Inhibition of ATF4 on NLRP3 Inflammasome Activation in Cerebral Ischemia-Reperfusion Injury in Rats
Berberine Ameliorates Spatial Learning Memory Impairment and Modulates Cholinergic Anti-Inflammatory Pathway in Diabetic Rats
Berberine Ameliorates Diabetes-Associated Cognitive Decline through Modulation of Aberrant Inflammation Response and Insulin Signaling Pathway in DM Rats
Melatonin inhibits the inflammation and apoptosis in rats with diabetic retinopathy via MAPK pathway.
Exposure to nickel oxide nanoparticles induces pulmonary inflammation through NLRP3 inflammasome activation in rats
Theaflavin alleviates inflammatory response and brain injury induced by cerebral hemorrhage via inhibiting the nuclear transcription factor kappa β-related pathway in rats
The activation of IL-17 signaling pathway promotes pyroptosis in pneumonia-induced sepsis
P2X7 Receptor (P2X7R) of Microglia Mediates Neuroinflammation by Regulating (NOD)-Like Receptor Protein 3 (NLRP3) Inflammasome-Dependent Inflammation After Spinal Cord Injury
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
11 Customer Q&As for Rat IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine®
Question
Is the detection antibody in EK0592 available apart from the kit, unconjugated? Are you able to reveal the clone ID, assuming it is monoclonal?
Verified customer
Asked: 2022-09-06
Answer
I am sorry to inform you that we don't sell detection antibody in EK0592 independently. Both the capture and detection antibodies in EK0592 are goat polyclonal antibodies.
Boster Scientific Support
Answered: 2022-09-07
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
W. Wilson
Verified customer
Asked: 2020-11-17
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-11-17
Question
Q: is it okay to use citrate plasma as samples in Rat IL-18 Picokine® ELISA Kit (Catalog # EK0592)?
Verified Customer
Verified customer
Asked: 2020-09-22
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of IL-18 causing disruption of its protein structure. IL-18 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the IL-18 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-09-22
Question
Has the serum / plasma MRD (Minimal Required Dilution) been calculated for EK0592 kit?
Verified customer
Asked: 2020-04-05
Answer
The serum / plasma MRD (Minimal Required Dilution) for Rat IL-18 ELISA Kit PicoKine EK0592 is 1:1.
Boster Scientific Support
Answered: 2020-04-07
Question
Q: can you suggest the dilution ratio of serum samples for detection of IL-18 in Rat serum? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
V. Mitchell
Verified customer
Asked: 2019-08-24
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL-18 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-08-24
Question
Was baculovirus expression system used for the manufacturing process of EK0592? How about recombinant Rat IL-18 standard and antibodies? Is this a recombinant protein?
Verified customer
Asked: 2019-05-15
Answer
For the Rat IL-18 ELISA Kit PicoKine™ (EK0592), E.coli expression system is used and it is a recombinant protein.
Boster Scientific Support
Answered: 2019-05-16
Question
Has EK0592 been validated on urine samples?
Verified customer
Asked: 2019-05-15
Answer
We have not tested nor validated the Rat IL-18 ELISA Kit PicoKine™ (EK0592) with urine sample. In theory the ELISA kit will work for all sample types if the target protein is present at a level that falls within the linear range of the ELISA kit detection range. For sample types that we have not tested for, we suggest to run pilot experiments and decide the optimal sample dilution.
Boster Scientific Support
Answered: 2019-05-16
Question
Q: Can IL-18 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2017-06-15
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2017-06-15
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
H. Williams
Verified customer
Asked: 2017-04-16
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2017-04-16
Question
Q: if the enzyme conjugated IL-18 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-18 antigen?
Verified Customer
Verified customer
Asked: 2016-03-19
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2016-03-19
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification IL-18 using the EK0592 Rat IL-18 Picokine® ELISA Kit?
D. Davis
Verified customer
Asked: 2014-01-28
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2014-01-28
