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Product Info Summary
| SKU: | PB9006 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-IL18 Antibody Picoband®
View all IL-18/IL-1F4 Antibodies
SKU/Catalog Number
PB9006
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL18 Antibody Picoband® catalog # PB9006. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL18 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9006)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human IL-18 recombinant protein (Position: Y37-D193). Human IL-18 shares 66% and 64% amino acid (aa) sequences identity with mouse and rat IL-18, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9006 is reactive to IL18 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
22 kDa
Calculated molecular weight
22326 MW
Background of IL-18/IL-1F4
Interleukin-18, also known as IL18, is a protein which in humans is encoded by the IL18 gene. IL-18 is a cytokine produced by macrophages and other cells that belongs to the IL-1 superfamily. It is mapped to 11q22.2-q22.3. IL-18 works by binding to the interleukin-18 receptor, and together with IL-12, it induces cell-mediated immunity following infection with microbial products like lipopolysaccharide (LPS). After stimulation with IL-18, natural killer (NK) cells and certain T cells release another important cytokine called interferon-γ (IFN-γ) or type II interferon that plays an important role in activating the macrophages or other cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production in B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9006 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Positive Control
WB: human Hela whole cell, human HepG2 whole cell
IHC: human tonsil tissue
ICC/IF: A549 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IL18 using anti-IL18 antibody (PB9006).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL18 antigen affinity purified polyclonal antibody (Catalog # PB9006) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL18 at approximately 22 kDa. The expected band size for IL18 is at 22 kDa.
Click image to see more details
Figure 2. IHC analysis of IL18 using anti-IL18 antibody (PB9006).
IL18 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL18 Antibody (PB9006) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IF analysis of IL18 using anti-IL18 antibody (PB9006) and anti-Beta Tubulin antibody (M01857-3).
IL18 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-IL18 Antibody (PB9006) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For IL18 (Source: Uniprot.org, NCBI)
Gene Name
IL18
Full Name
Interleukin-18
Weight
22326 MW
Superfamily
IL-1 family
Alternative Names
Interleukin-18;IL-18;Iboctadekin;Interferon gamma-inducing factor;IFN-gamma-inducing factor;Interleukin-1 gamma;IL-1 gamma;IL18;IGIF, IL1F4; IL18 IGIF, IL-18, IL-1g, IL1F4 interleukin 18 interleukin-18|IFN-gamma-inducing factor|IL-1 gamma|iboctadekin|interleukin 18 (interferon-gamma-inducing factor)|interleukin-1 gamma
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL18, check out the IL18 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL18: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL18 Antibody Picoband® (PB9006)
Hello CJ!
PB9006 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The effect of salusin-? on expression of pro- and anti-inflammatory cytokines in human umbilical vein endothelial cells (HUVECs)
Yang Yj, Shen Y, Chen Sh, Ge Xr. World J Gastroenterol. 2005 Aug 7;11(29):4524-9. Role Of Interleukin 18 In Acute Lung Inflammation Induced By Gut Ischemia Reperfusion.
Wang Y, Zhang X, Zhang Y, Xu H, Fang G. J Reprod Immunol. 2011 Dec;92(1-2):45-53. Doi: 10.1016/J.Jri.2011.07.002. Epub 2011 Sep 13. Expression And Localization Of Il-18 In The Hypothalamic-Pituitary-Ovarian Axis Of Non-Pregnant, Pregnant, And Abor...
Si Lf, Zhang Sy, Gao Cs, Chen Sl, Zhao J, Cheng Xc. Asian-Australas J Anim Sci. 2013 Oct;26(10):1399-405. Doi: 10.5713/Ajas.2013.13101. Effects Of Ifn-?? On Il-18 Expression In Pregnant Rats And Pregnancy Outcomes.
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Customer Reviews
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Customer Q&As
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19 Customer Q&As for Anti-IL18 Antibody Picoband®
Question
Since the mature form of IL-18 was used as immunogen for PB9006, have you ever done any internal testing about its reacitivity with the active form after processing by CASP?
Verified customer
Asked: 2020-11-09
Answer
For the Anti-IL18 Antibody Picoband | Bosterbio (PB9006), our lab hasn't validated reacitivity with the active form after processing by CASP.
Boster Scientific Support
Answered: 2020-11-10
Question
Is a blocking peptide available for product anti-IL18 antibody (PB9006)?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
We do provide the blocking peptide for product anti-IL18 antibody (PB9006). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-03
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for peripheral blood using anti-IL18 antibody PB9006. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-20
Question
What form of IL-18 does PB9006 detect?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
According to the molecular weight of the target band our lab technicians detected, 22kD, PB9006 detects the native pro-form of IL-18. <br> The molecular weight of mature form of IL-18 is 18KD.
Boster Scientific Support
Answered: 2019-10-29
Question
I was wanting to use your anti-IL18 antibody for ICC for human peripheral blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human peripheral blood identification?
Verified Customer
Verified customer
Asked: 2019-09-12
Answer
As indicated on the product datasheet, PB9006 anti-IL18 antibody has been tested for IHC-P, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human peripheral blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-12
Question
We have seen staining in human liver. Do you have any suggestions? Is anti-IL18 antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2019-07-23
Answer
From literature liver does express IL18. From Uniprot.org, IL18 is expressed in lower esophagus mucosa, liver, urinary bladder, peripheral blood, among other tissues. Regarding which tissues have IL18 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 8666798, 15326478
Peripheral blood, Pubmed ID: 10653850
Urinary bladder, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-07-23
Question
I am interested in to test anti-IL18 antibody PB9006 on human peripheral blood for research purposes, then I may be interested in using anti-IL18 antibody PB9006 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
The products we sell, including anti-IL18 antibody PB9006, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-08
Question
Is this PB9006 anti-IL18 antibody reactive to the isotypes of IL18?
Verified Customer
Verified customer
Asked: 2019-02-14
Answer
The immunogen of PB9006 anti-IL18 antibody is E.coli-derived human IL-18 recombinant protein (Position: Y37-D193). Human IL-18 shares 66% and 64% amino acid (aa) sequences identity with mouse and rat IL-18, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-02-14
Question
Will anti-IL18 antibody PB9006 work for ICC with peripheral blood?
Verified Customer
Verified customer
Asked: 2019-01-03
Answer
According to the expression profile of peripheral blood, IL18 is highly expressed in peripheral blood. So, it is likely that anti-IL18 antibody PB9006 will work for ICC with peripheral blood.
Boster Scientific Support
Answered: 2019-01-03
Question
Can you help my question with product PB9006, anti-IL18 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-10-30
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9006 anti-IL18 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-10-30
Question
Is there a BSA free version of anti-IL18 antibody PB9006 available?
Verified Customer
Verified customer
Asked: 2018-04-26
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-IL18 antibody PB9006 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-04-26
Question
We purchased anti-IL18 antibody for IHC-P on peripheral blood a few months ago. I am using human, and We intend to use the antibody for WB next. My question regards examining peripheral blood as well as lower esophagus mucosa in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2018-02-14
Answer
I looked at the website and datasheets of our anti-IL18 antibody and I see that PB9006 has been validated on human in both IHC-P and WB. Thus PB9006 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-02-14
Question
We were well pleased with the WB result of your anti-IL18 antibody. However we have been able to see positive staining in urinary bladder secreted. using this antibody. Is that expected? Could you tell me where is IL18 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-10-27
Answer
From what I have seen in literature, urinary bladder does express IL18. Generally IL18 expresses in secreted. Regarding which tissues have IL18 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 8666798, 15326478
Peripheral blood, Pubmed ID: 10653850
Urinary bladder, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-10-27
Question
I am Fei He as a FAS from Abcam. I am sorry to bother you. One of our customer wants to know the targets of Anti-IL-18 antibody (PB9006). Anti-IL18 antibody (ab191152) was raised against the mature human IL-18 (Y37-D193), did you test whether tested this Ab is able to detect the native pro-form of IL-18? By the way, do you have any data showing the specificity of this Ab on native mature IL-8? Thanks in advance for your help.
Verified Customer
Verified customer
Asked: 2017-10-12
Answer
According to the molecular weight of the target band our lab technicians detected, PB9006 detects the native pro-form of IL-18. https://www.bosterbio.com/anti-il-18-picoband-trade-antibody-pb9006-boster.html?options=cart The molecular weight of mature form of IL-18 is 18KD.
Boster Scientific Support
Answered: 2017-10-12
Question
I was wanting to use using your anti-IL18 antibody for positive regulation of macrophage derived foam cell differentiation studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-07-21
Answer
We appreciate your inquiry. This PB9006 anti-IL18 antibody is tested on hela whole cell lysate. It is guaranteed to work for IHC-P, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-07-21
Question
Would PB9006 anti-IL18 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-05-31
Answer
You can see on the product datasheet, PB9006 anti-IL18 antibody as been tested on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-05-31
Question
I see that the anti-IL18 antibody PB9006 works with ICC, what is the protocol used to produce the result images on the product page?
V. Carter
Verified customer
Asked: 2015-04-15
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-04-15
Question
We are currently using anti-IL18 antibody PB9006 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?
J. Thomas
Verified customer
Asked: 2015-01-09
Answer
The anti-IL18 antibody (PB9006) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-01-09
Question
Here is the WB image, lot number and protocol we used for peripheral blood using anti-IL18 antibody PB9006. Please let me know if you require anything else.
B. Miller
Verified customer
Asked: 2014-10-31
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-10-31
