Product Info Summary
SKU: | PROTP00375 |
---|---|
Size: | Starting from 20ug |
Source: | E. coli |
Application: | Enzyme Activity, SDS-PAGE |
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Product info
Product Name
Mouse DHFR Recombinant Protein
View all Dihydrofolate Reductase/DHFR recombinant proteins
SKU/Catalog Number
PROTP00375
Size
Starting from 20ug
Tag
His-Tag
Description
Mouse DHFR Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-187aa. Application(s): Enzyme Activity, SDS-PAGE. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method).
Storage & Handling
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Cite This Product
Mouse DHFR Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP00375)
Form
Liquid
Formulation
20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.
Concentration
1mg/ml (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Predicted MW
23.8 kDa (207aa) confirmed by MALDI-TOF
Amino Acid Sequence
Mouse, P00375, 1-187aa;Bioactivity
Specific activity is > 2000 pmol/min/ug, and is defined as the amount of enzyme that converts 1.0 pmole of dihydrofolic acid to tetrahydrofolic acid per minute at pH 6.5 at 25°C.Assay dilution & Images
Validation Images & Assay Conditions
Click image to see more details
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Protein Target Info & Infographic
Gene/Protein Information For Dhfr (Source: Uniprot.org, NCBI)
Gene Name
Dhfr
Full Name
Dihydrofolate reductase
Weight
23.8 kDa (207aa) confirmed by MALDI-TOF
Superfamily
dihydrofolate reductase family
Alternative Names
Dihydrofolate reductase, EC 1.5.1.3 DHFR DHFRP1, DYR dihydrofolate reductase dihydrofolate reductase
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on Dhfr, check out the Dhfr Infographic
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In this infographic, you will see the following information for Dhfr: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For Mouse DHFR Recombinant Protein (PROTP00375)
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