Mouse DHFR Recombinant Protein

Dihydrofolate Reductase/DHFR protein,

Mouse DHFR Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-187aa. Application(s): Enzyme Activity, SDS-PAGE. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method).

Product Info Summary

SKU: PROTP00375
Size: Starting from 20ug
Source: E. coli
Application: Enzyme Activity, SDS-PAGE

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Product Name

Mouse DHFR Recombinant Protein

View all Dihydrofolate Reductase/DHFR recombinant proteins

SKU/Catalog Number

PROTP00375

Size

Starting from 20ug

Tag

His-Tag

Description

Mouse DHFR Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-187aa. Application(s): Enzyme Activity, SDS-PAGE. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method).

Storage & Handling

Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles.

Cite This Product

Mouse DHFR Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP00375)

Form

Liquid

Formulation

20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.

Concentration

1mg/ml (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Predicted MW

23.8 kDa (207aa) confirmed by MALDI-TOF

Amino Acid Sequence

Mouse, P00375, 1-187aa; MVRPLNCIVA VSQNMGIGKN GDLPWPPLRN EFKYFQRMTT TSSVEGKQNL VIMGRKTWFS IPEKNRPLKD RINIVLSREL KEPPRGAHFL AKSLDDALRL IEQPELASKV DMVWIVGGSS VYQEAMNQPG HLRLFVTRIM QEFESDTFFP EIDLGKYKLL PEYPGVLSEV QEEKGIKYKF EVYEKKD

Bioactivity

Specific activity is > 2000 pmol/min/ug, and is defined as the amount of enzyme that converts 1.0 pmole of dihydrofolic acid to tetrahydrofolic acid per minute at pH 6.5 at 25°C.

Validation Images & Assay Conditions

Gene/Protein Information For DHFR (Source: Uniprot.org, NCBI)

Gene Name

DHFR

Full Name

Dihydrofolate reductase

Weight

23.8 kDa (207aa) confirmed by MALDI-TOF

Superfamily

dihydrofolate reductase family

Alternative Names

DHFR; DHFRP1; Dihydrofolate Reductase; DYR; EC 1.5.1.3 DHFR DHFRP1, DYR dihydrofolate reductase dihydrofolate reductase

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on DHFR, check out the DHFR Infographic

DHFR infographic

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In this infographic, you will see the following information for DHFR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].

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Size

Total: $250

SKU:PROTP00375

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Lead time for this item is typically 7-10 days

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