Human Dihydrofolate Reductase/DHFR Recombinant Protein

Dihydrofolate Reductase/DHFR protein,

Human Dihydrofolate Reductase/DHFR Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-187aa. Application(s): SDS-PAGE, Enzyme Activity.

Product Info Summary

SKU: PROTP00374
Size: Starting from 20ug
Source: E. coli
Application: Enzyme Activity, SDS-PAGE

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Product Name

Human Dihydrofolate Reductase/DHFR Recombinant Protein

View all Dihydrofolate Reductase/DHFR recombinant proteins

SKU/Catalog Number

PROTP00374

Size

Starting from 20ug

Tag

His-Tag

Description

Human Dihydrofolate Reductase/DHFR Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-187aa. Application(s): SDS-PAGE, Enzyme Activity.

Storage & Handling

Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)

Cite This Product

Human Dihydrofolate Reductase/DHFR Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP00374)

Form

Liquid

Formulation

20mM Tris-HCl buffer (pH 8.0) containing 0.1M Nacl 2mM DTT, and 30% glycerol

Concentration

1mg/ml (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Predicted MW

23.6 kDa (207aa) confirmed by MALDI-TOF

Amino Acid Sequence

Human, P00374, 1-187aa; MVGSLNCIVA VSQNMGIGKN GDLPWPPLRN EFRYFQRMTT TSSVEGKQNL VIMGKKTWFS IPEKNRPLKG RINLVLSREL KEPPQGAHFL SRSLDDALKL TEQPELANKV DMVWIVGGSS VYKEAMNHPG HLKLFVTRIM QDFESDTFFP EIDLEKYKLL PEYPGVLSDV QEEKGIKYKF EVYEKND

Bioactivity

Specific activity is > 2000 pmol/min/ug, and is defined as the amount of enzyme that converts 1.0 pmole of dihydrofolic acid to tetrahydrofolic acid per minute at pH 6.5 at 25°C.

Validation Images & Assay Conditions

Gene/Protein Information For DHFR (Source: Uniprot.org, NCBI)

Gene Name

DHFR

Full Name

Dihydrofolate reductase

Weight

23.6 kDa (207aa) confirmed by MALDI-TOF

Superfamily

dihydrofolate reductase family

Alternative Names

Dihydrofolate reductase, EC 1.5.1.3 DHFR DHFRP1, DYR dihydrofolate reductase dihydrofolate reductase

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on DHFR, check out the DHFR Infographic

DHFR infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for DHFR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].

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Size

Total: $234

SKU:PROTP00374

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