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Product Info Summary
| SKU: | A04540 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-DARC ACKR1 Antibody
SKU/Catalog Number
A04540
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-DARC ACKR1 Antibody (Catalog # A04540). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
DARC antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-DARC ACKR1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04540)
Host
Rabbit
Contents
DARC Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
DARC antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human DARC. The immunogen is located within the last 50 amino acids of DARC.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least three isoforms are known to exist; this antibody will recognize all three. ZIMP10 antibody is predicted to not cross-react with other PIAS protein family members.
Reactive Species
A04540 is reactive to DARC in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
35553 MW
Background of DARC
DARC, also known as the Duffy antigen/chemokine receptor, is a seven-transmembrane protein homologous to the classical chemokine G-protein coupled receptors (GPCRs) with the exception of the motif required for G protein coupling. DARC can bind with high affinity several chemokines without transducing any signal, suggesting it may modulate the signals normally induced by these chemokines. Recently, DARC was found to interact with KAI1, a four transmembrane protein recently identified as a tumor metastasis suppressor protein. It is thought that tumor cells dislodged from the primary tumor and expressing KAI1 interact with DARC proteins expressed on vascular cells, transmitting a senescent signal to the tumor cells, while tumor cells that have lost KAI1 expression can proliferate and potentially give rise to metastases. At least three isoforms of DARC are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04540 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
DARC antibody can be used for detection of DARC by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of DARC in human cerebellum tissue lysate with DARC antibody at 1 μg/mL.
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Immunohistochemistry of DARC in mouse brain tissue with DARC antibody at 2.5 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For DARC (Source: Uniprot.org, NCBI)
Gene Name
DARC
Full Name
Atypical chemokine receptor 1
Weight
35553 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
FY, Dfy, GPD, GpFy, ACKR1, CCBP1, CD234, WBCQ1, FY, Atypical chemokine receptor 1 ACKR1 CCBP1, CD234, DARC, DARC/ACKR1, Dfy, FY, GPD, GpFy, WBCQ1 atypical chemokine receptor 1 (Duffy blood group) atypical chemokine receptor 1|Duffy chemokine receptor|Duffy blood group |Duffy blood group system protein|Duffy blood group, chemokine receptor|Fy glycoprotein|glycoprotein D|plasmodium vivax receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DARC, check out the DARC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DARC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DARC ACKR1 Antibody (A04540)
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