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Product Info Summary
| SKU: | FEK1287 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Mouse CD6 PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK1287
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse soluble CD6 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Mouse Cd6 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Mouse CD6 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1287)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: L18-G396
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK1287 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK1287 is reactive to CD6 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
FEK1287 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD6
CD6 (Cluster of Differentiation 6) is a human protein encoded by the CD6 gene. It is mapped to 11q12.2. CD6 is expressed predominantly in peripheral T cells and mature medullary thymocytes. This gene encodes a protein found on the outer membrane of T-lymphocytes as well as some other immune cells. The encoded protein contains three scavenger receptor cysteine-rich (SRCR) domains and a binding site for an activated leukocyte cell adhesion molecule. The gene product is important for continuation of T cell activation. What’s more, this gene may be associated with susceptibility to multiple sclerosis.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK1287-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK1287-ST | Mouse CD6 Standard | 2 | 10 ng/tube |
| FEK1287-A | Mouse CD6 Antibody A | 1 | 3ml |
| FEK1287-B | Mouse CD6 Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 | |
| O.D. | 0.014 | 0.059 | 0.1 | 0.185 | 0.349 | 0.666 | 1.217 | 2.030 |
Data Example Images
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Mouse CD6 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse CD6 PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 133 | 525 | 1268 | 129 | 485 | 1288 |
| Standard deviation | 5.58 | 38.9%7 | 92.56 | 6.45 | 37.83 | 114.63 |
| CV (%) | 4.2% | 6.7% | 7.3% | 5% | 7.8% | 8.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CD6 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 133 | 133 | 120 | 119 | 126 | 6.75 | 5.3% |
| Sample 2 | 525 | 507 | 540 | 469 | 510 | 26.52 | 5.2% |
| Sample 3 | 1268 | 1267 | 1220 | 1339 | 1273 | 42.5 | 3.3% |
Protein Target Info & Infographic
Gene/Protein Information For CD6 (Source: Uniprot.Org, NCBI)
Gene Name
CD6
Full Name
T-cell differentiation antigen CD6
Weight
71.801kDa
Alternative Names
CD6 antigenFLJ44171; CD6 molecule; CD6; T12; Tp120; TP120T-cell differentiation antigen CD6 CD6 TP120 CD6 molecule T-cell differentiation CD6|CD6 |T12
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CD6, check out the CD6 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CD6: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse CD6 PicoKine® Quick ELISA Kit (FEK1287)
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