Product Info Summary
SKU: | LS003556 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of interleukin 1 receptor accessory protein (IL1RAP), transcript variant 1 |
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Product info
Product Name
IL1RAP (NM_002182) Human Over-expression Lysate
See all IL-1 RAcP/IL-1 R3 products
SKU/Catalog Number
LS003556
Size
100 µg
Description
Transient overexpression lysate of interleukin 1 receptor accessory protein (IL1RAP), transcript variant 1
Storage & Handling
The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Cite This Product
IL1RAP (NM_002182) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS003556)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
![ls003556 il1rap human over expression lysates nm 001167928 wb testing 3 ls003556 il1rap human over expression lysates nm 001167928 wb testing 3](https://www.bosterbio.com/media/catalog/product/l/s/ls003556-il1rap-human-over-expression-lysates-nm-001167928-wb-testing-3.jpg)
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IL1RAP Human Over-expression Lysates NM_001167928.
Western blot validation of overexpression lysate (LS003556) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with IL1RAP (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
![ls003556 il1rap human over expression lysates nm 002182 wb testing 1 ls003556 il1rap human over expression lysates nm 002182 wb testing 1](https://www.bosterbio.com/media/catalog/product/l/s/ls003556-il1rap-human-over-expression-lysates-nm-002182-wb-testing-1.jpg)
Click image to see more details
IL1RAP Human Over-expression Lysates NM_002182.
Western blot validation of overexpression lysate (LS003556) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with IL1RAP (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
![ls003556 il1rap human over expression lysates nm 134470 wb testing 2 ls003556 il1rap human over expression lysates nm 134470 wb testing 2](https://www.bosterbio.com/media/catalog/product/l/s/ls003556-il1rap-human-over-expression-lysates-nm-134470-wb-testing-2.jpg)
Click image to see more details
IL1RAP Human Over-expression Lysates NM_134470.
Western blot validation of overexpression lysate (LS003556) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with IL1RAP (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for IL1RAP (Source: Uniprot.org, NCBI)
Gene Name
IL1RAP
Full Name
Interleukin-1 receptor accessory protein
Weight
Superfamily
interleukin-1 receptor family
Alternative Names
C3orf13IL-1RAcPIL1R3Interleukin-1 receptor 3; FLJ37788; IL-1 R3; IL-1 RAcP; IL-1 receptor accessory protein; IL-1R3; IL-1R-3; IL1RAcP; IL-1RAcP; IL1RAP; interleukin 1 receptor accessory protein; interleukin-1 receptor accessory protein beta; interleukin-1 receptor accessory protein IL1RAP C3orf13, IL-1RAcP, IL1R3 interleukin 1 receptor accessory protein interleukin-1 receptor accessory protein|IL-1 receptor accessory protein|interleukin-1 receptor 3|interleukin-1 receptor accessory protein beta
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on IL1RAP, check out the IL1RAP Infographic
![IL1RAP infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL1RAP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For IL1RAP (NM_002182) Human Over-expression Lysate (LS003556)
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