Product Info Summary
SKU: | PROTP01583 |
---|---|
Size: | 2ug, 10ug, 0.1mg |
Origin Species: | Human |
Source: | HEK |
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Product info
Product Name
IL1A Human, Interleukin-1 alpha Human Recombinant Protein, HEK
View all IL-1 alpha/IL-1F1 recombinant proteins
SKU/Catalog Number
PROTP01583
Size
2ug, 10ug, 0.1mg
Description
Interleukin-1 alpha Human Recombinant produced in HEK cells is a glycosylated monomer, having a molecular weight of 18kDa due to glycosylation. The IL1A is purified by proprietary chromatographic techniques.
Storage & Handling
Lyophilized IL-1A although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL1A should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Cite This Product
IL1A Human, Interleukin-1 alpha Human Recombinant Protein, HEK (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP01583)
Form
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The IL-1A protein was lyophilized from a 0.2µm filtered solution of PBS pH 7.4 with 10% trehalose as protectant.
Purity
Greater than 97.0% as determined by SDS-PAGE.
Predicted MW
30.607kDa
Reconstitution
It is recommended to reconstitute the lyophilized IL1A in deionized water to a stock solution of 0.5mg/ml.
Biological Activity
Assay # 1: The biological activity was measured by its binding ability in a functional ELISA, the ED50 is typically 0.5-5 µg/ml. Assay # 2: The biological activity was determined by the dose-dependent stimulation of the proliferation of mouse D10S cells, the ED50 is typically less than 1pg/ml, corresponding to a specific activity of greater than 1x109 unit/mg.
Assay dilution & Images
Reconstitution
It is recommended to reconstitute the lyophilized IL1A in deionized water to a stock solution of 0.5mg/ml.
Validation Images & Assay Conditions
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Protein Target Info & Infographic
Gene/Protein Information For IL1A (Source: Uniprot.org, NCBI)
Gene Name
IL1A
Full Name
Interleukin-1 alpha
Weight
30.607kDa
Superfamily
IL-1 family
Alternative Names
Hematopoietin-1; Lymphocyte-activating factor (LAF); Endogenous Pyrogen (EP); Leukocyte Endogenous Mediator (LEM); Mononuclear Cell Factor (MCF); IL-1 alpha;IL1; IL-1A; IL1F1 IL1A IL-1 alpha, IL-1A, IL1, IL1-ALPHA, IL1F1 interleukin 1 alpha interleukin-1 alpha|hematopoietin-1|preinterleukin 1 alpha|pro-interleukin-1-alpha
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on IL1A, check out the IL1A Infographic
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In this infographic, you will see the following information for IL1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For IL1A Human, Interleukin-1 alpha Human Recombinant Protein, HEK (PROTP01583)
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