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Product Info Summary
| SKU: | EK0846 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants and cell lysates. |
Product info
Product Name
Human TrkA ELISA Kit PicoKine®
SKU/Catalog Number
EK0846
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human TrkA ELISA Kit PicoKine® (96 Tests). Quantitate Human NTRK1 in cell culture supernatants and cell lysates. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human TrkA ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0846)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: A33-E407
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0846 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0846 is reactive to NTRK1 in Human samples
Validated Sample Types
cell culture supernatants and cell lysates.
Application Guarantee
EK0846 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TrkA
Tropomyosin receptor kinase A (TrkA) are efficacious in attenuating skeletal pain.1 TrkA mutants were able to activate signaling cascades and were even more efficient in promoting neurite outgrowth than the wild-type receptor.2 TrkA is part of a sub-family of protein kinases which includes TrkB and TrkC. Also, there are other neurotrophic factors structurally related to NGF: BDNF (for Brain-Derived Neurotrophic Factor), NT-3 (for Neurotrophin-3) and NT-4 (for Neurotrophin-4). While TrkA mediates the effects of NGF, TrkB is bound and activated by BDNF, NT-4, and NT-3. Further, TrkC binds and is activated by NT-3.3 TrkA receptor was found in kehumanoconus-affected corneas, along with an increased level of repressor isoform of Sp3 transcription factor.4
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0846-CAP | Anti-Human NTRK1 Pre-coated 96-well strip microplate | 1 |
| EK0846-ST | Human NTRK1 Standard | 2 vials, 10 ng/tube |
| EK0846-DA | Human NTRK1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 125000 | 25000 | 5000 | 10000 |
| O.D. | 0.060 | 0.100 | 0.137 | 0.202 | 0.353 | 0.625 | 1.119 | 1.923 |
Data Example Images
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Human TrkA PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human TrkA ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 314 | 1718 | 5724 | 303 | 1722 | 6073 |
| Standard deviation | 22.29 | 92.77 | 360.61 | 25.45 | 117.09 | 406.89 |
| CV (%) | 7.1% | 5.4% | 6.8%% | 8.4% | 6.8% | 6.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of TrkA in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 314 | 293 | 280 | 296 | 295 | 12.13 | 4.1% |
| Sample 2 | 1718 | 1671 | 1601 | 1714 | 1676 | 47.05 | 2.8% |
| Sample 3 | 5724 | 5182 | 5771 | 4794 | 5367 | 404.1 | 7.5% |
Protein Target Info & Infographic
Gene/Protein Information For NTRK1 (Source: Uniprot.Org, NCBI)
Gene Name
NTRK1
Full Name
High affinity nerve growth factor receptor
Weight
87497 MW
Superfamily
protein kinase superfamily
Alternative Names
High affinity nerve growth factor receptor;2.7.10.1;Neurotrophic tyrosine kinase receptor type 1;TRK1-transforming tyrosine kinase protein;Tropomyosin-related kinase A;Tyrosine kinase receptor;Tyrosine kinase receptor A;Trk-A;gp140trk;p140-TrkA;NTRK1;MTC, TRK, TRKA; NTRK1 MTC, TRK, TRK1, TRKA, Trk-A, p140-TrkA neurotrophic receptor tyrosine kinase 1 high affinity nerve growth factor receptor|Oncogene TRK|TRK1-transforming tyrosine kinase protein|gp140trk|neurotrophic tyrosine kinase, receptor, type 1|tropomyosin-related kinase A|tyrosine kinase receptor A
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on NTRK1, check out the NTRK1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for NTRK1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human TrkA ELISA Kit PicoKine® (EK0846)
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Customer Reviews
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Customer Q&As
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9 Customer Q&As for Human TrkA ELISA Kit PicoKine®
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of TrkA in Human cell lysates? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2020-08-20
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the TrkA ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-08-20
Question
Q: What is the optimal O.D. value for TrkA ELISA kit? I used your TrkA ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain TrkA even though the O.D. values are not very high?
K. Perez
Verified customer
Asked: 2020-08-05
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this TrkA ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of TrkA that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2020-08-05
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
P. Hall
Verified customer
Asked: 2019-06-06
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-06-06
Question
Q: Can TrkA ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2018-05-18
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-05-18
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2018-05-02
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-05-02
Question
Q: how to proceed with the analysis of ELISA data? I measured TrkA level in cell lysates.
Verified Customer
Verified customer
Asked: 2016-11-04
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2016-11-04
Question
Q: which procedure should I follow in order to thaw whole blood sample for TrkA ELISA after freezing?
Verified Customer
Verified customer
Asked: 2016-04-06
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test TrkA for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2016-04-06
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
W. Collins
Verified customer
Asked: 2014-10-28
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2014-10-28
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis TrkA using the EK0846 Human TrkA Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2014-07-19
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2014-07-19
