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Product Info Summary
| SKU: | EK1472 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human SFRP5/Sarp3 ELISA Kit PicoKine®
SKU/Catalog Number
EK1472
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human SFRP5/Sarp3 ELISA Kit PicoKine® (96 Tests). Quantitate Human SFRP5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human SFRP5/Sarp3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1472)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: CHO; Immunogen sequence: E30-H317
Sensitivity
<15 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1472 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1472 is reactive to SFRP5 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1472 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of sFRP-5
Secreted frizzled-related protein 5 is a protein that in humans is encoded by the SFRP5 gene. SFRP5 is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1472-CAP | Anti-Human SFRP5 Pre-coated 96-well strip microplate | 1 |
| EK1472-ST | Human SFRP5 Standard | 2 vials, 10 ng/tube |
| EK1472-DA | Human SFRP5 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.087 | 0.149 | 0.216 | 0.390 | 0.789 | 1.226 | 1.896 | 2.419 |
Data Example Images
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Human SFRP5 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect sFRP-5, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 156 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human SFRP5/Sarp3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 329 | 1068 | 4969 | 309 | 1159 | 5218 |
| Standard deviation | 20.39 | 69.42 | 203.72 | 21.93 | 96.19 | 250.46 |
| CV (%) | 6.2% | 6.5% | 4.1% | 7.1% | 8.3% | 4.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of sFRP-5 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 329 | 353 | 327 | 347 | 339 | 11.22 | 3.3% |
| Sample 2 | 1068 | 1216 | 1033 | 1211 | 1132 | 82.45 | 7.2% |
| Sample 3 | 4969 | 5003 | 5645 | 5635 | 5313 | 327.23 | 6.1% |
Protein Target Info & Infographic
Gene/Protein Information For SFRP5 (Source: Uniprot.Org, NCBI)
Gene Name
SFRP5
Full Name
Secreted frizzled-related protein 5
Weight
35563 MW
Superfamily
secreted frizzled-related protein (sFRP) family
Alternative Names
Secreted frizzled-related protein 5;sFRP-5;Frizzled-related protein 1b;FRP-1b;Secreted apoptosis-related protein 3;SARP-3;SFRP5;FRP1B, SARP3; SFRP5 SARP3 secreted frizzled related protein 5 secreted frizzled-related protein 5|FRP-1b|SARP-3|frizzled-related protein 1b|sFRP-5|secreted apoptosis related protein 3
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on SFRP5, check out the SFRP5 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SFRP5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human SFRP5/Sarp3 ELISA Kit PicoKine® (EK1472)
Hello CJ!
EK1472 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Reduction of Secreted Frizzled-Related Protein 5 Drives Vascular Calcification through Wnt3a-Mediated Rho/ROCK/JNK Signaling in Chronic Kidney Disease
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Human SFRP5/Sarp3 ELISA Kit PicoKine®
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification sFRP-5 using the EK1472 Human sFRP-5 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-10-09
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2020-10-09
Question
Q: can I use citrate plasma as samples in Human sFRP-5 Picokine® ELISA Kit (Catalog # EK1472)?
Verified Customer
Verified customer
Asked: 2019-03-26
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of sFRP-5 causing disruption of its protein structure. sFRP-5 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the sFRP-5 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-03-26
Question
Q: how to thaw whole blood sample for sFRP-5 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-03-18
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test sFRP-5 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2019-03-18
Question
Q: how to proceed with the analysis of ELISA data? I have performed sFRP-5 level in serum.
Verified Customer
Verified customer
Asked: 2018-10-27
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-10-27
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
B. Surve
Verified customer
Asked: 2018-05-26
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-05-26
Question
Q: if the enzyme conjugated sFRP-5 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the sFRP-5 antigen?
Verified Customer
Verified customer
Asked: 2018-05-17
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-05-17
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of sFRP-5 in Human serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-05-17
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the sFRP-5 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-05-17
