Human TIMP-2 PicoKine® Quick ELISA Kit

Introduction

Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Product Name

Human TIMP-2 PicoKine® Quick ELISA Kit

See all TIMP-2 products

SKU/Catalog Number

FEK0522

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human TIMP-2 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human TIMP2 in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human TIMP-2 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0522)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: CHO; Immunogen sequence: C27-P220

Sensitivity

<2 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

See datasheet of FEK0522 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0522 is reactive to TIMP2 in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.

Application Guarantee

FEK0522 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of TIMP-2

TIMP2 gen is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP2 is 83 kilobase pairs (kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. The standard product used in this kit is recombinant human TIMP-2 with the molecular mass of 22Kda and 194 amino acid.

Kit Components

Catalog number	Description	Quantity
FEK0522-CAP	96-well plate precoated with anti-Human TIMP-2 antibody	1
EK0522-ST	lyophilized recombinant Human TIMP-2 standard	10 ng/tube
FEK0522-DA	Biotinylated anti-Human TIMP-2 detection antibody (50x)	100 ul
-	Avidin-Biotin-Peroxidase Complex (30x)	400 µl
AR1106-1	Sample diluent	30 ml
AR1106-2	Antibody diluent	12 ml
-	Avidin-Biotin-Peroxidase Diluent	12 ml
AR1106-5	Wash Buffer (25x)	20 ml
AR1104	Color Developing Reagent (TMB)	10 ml
AR1105	Stop Solution	10 ml
PLA-SEA	Plate Sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	156	312	625	125000	25000	5000	10000
O.D.	0.005	0.109	0.198	0.418	0.787	1.397	2.109	2.513

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human TIMP-2 PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of TIMP-2 in ELISA kits from four different production batches/lots.

Gene/Protein Information For TIMP2 (Source: Uniprot.Org, NCBI)

Alternative Names

Metalloproteinase inhibitor 2;CSC-21K;Tissue inhibitor of metalloproteinases 2;TIMP-2;TIMP2; TIMP2 CSC-21K, DDC8 TIMP metallopeptidase inhibitor 2 metalloproteinase inhibitor 2|testicular secretory protein Li 57|tissue inhibitor of metalloproteinases 2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis