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Product Info Summary
| SKU: | EK0522 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. |
Product info
Product Name
Human TIMP-2 ELISA Kit PicoKine®
SKU/Catalog Number
EK0522
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human TIMP-2 ELISA Kit PicoKine® (96 Tests). Quantitate Human TIMP2 in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human TIMP-2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0522)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: CHO; Immunogen sequence: C27-P220
Sensitivity
<2 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0522 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0522 is reactive to TIMP2 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.
Application Guarantee
EK0522 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TIMP-2
TIMP2 gen is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP2 is 83 kilobase pairs (kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. The standard product used in this kit is recombinant human TIMP-2 with the molecular mass of 22Kda and 194 amino acid.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0522-CAP | Anti-Human TIMP2 Pre-coated 96-well strip microplate | 1 |
| EK0522-ST | Human TIMP2 Standard | 2 vials, 10 ng/tube |
| EK0522-DA | Human TIMP2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.005 | 0.051 | 0.109 | 0.198 | 0.418 | 0.787 | 1.297 | 1.909 |
Data Example Images
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Human TIMP-2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect TIMP-2, Dilution ratio of 1:100, concentration in serum and plasma is 18-393 ng/ml..
Some articles we found to cite concentrations of TIMP-2 in samples: 24520285 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human TIMP-2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 249 | 1786 | 4399 | 239 | 1654 | 4356 |
| Standard deviation | 17.43 | 94.65 | 325.52 | 16.73 | 112.47 | 361.54 |
| CV (%) | 6.4% | 5.3% | 7.4% | 7% | 6.8% | 8.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of TIMP-2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 249 | 258 | 275 | 291 | 268 | 16.11 | 6% |
| Sample 2 | 1786 | 1798 | 1796 | 2014 | 1848 | 95.65 | 5.1% |
| Sample 3 | 4399 | 5066 | 4506 | 5016 | 4746 | 297.19 | 6.2% |
Protein Target Info & Infographic
Gene/Protein Information For TIMP2 (Source: Uniprot.Org, NCBI)
Gene Name
TIMP2
Full Name
Metalloproteinase inhibitor 2
Weight
24399 MW
Superfamily
protease inhibitor I35 (TIMP) family
Alternative Names
Metalloproteinase inhibitor 2;CSC-21K;Tissue inhibitor of metalloproteinases 2;TIMP-2;TIMP2; TIMP2 CSC-21K, DDC8 TIMP metallopeptidase inhibitor 2 metalloproteinase inhibitor 2|testicular secretory protein Li 57|tissue inhibitor of metalloproteinases 2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TIMP2, check out the TIMP2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TIMP2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human TIMP-2 ELISA Kit PicoKine® (EK0522)
Hello CJ!
EK0522 has been cited in 14 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Gelatinases and their tissue inhibitors in a group of subjects with obstructive sleep apnea syndrome
Behaviour of the plasma concentration of gelatinases and their tissue inhibitors in subjects with venous leg ulcers
Gelatinases and Their Tissue Inhibitors in a Group of Subjects With Metabolic Syndrome
MMP-2, MMP-9, TIMP-1, and TIMP-2 in the Peripheral Blood of Patients with Differentiated Thyroid Carcinoma
Expression pattern of matrix metalloproteinases-2 and -9 and their tissue inhibitors in patients with chronic inflammatory demyelinating polyneuropathy
Implication of MMP-9 and urokinase plasminogen activator (uPA) in the activation of pro-matrix metalloproteinase (MMP)-13
Cosentino G,Di Stefano V,Presti RL,Montana M,Todisco M,Gastaldi M,Cortese A,Alfonsi E,Tassorelli C,Fierro B,Caimi G,Brighina F.Expression pattern of matrix metalloproteinases-2 and -9 and their tissue inhibitors in patients with chronic inflammatory demyelinating polyneuropathy.Neurol Sci.2021 May 14.doi:10.1007/s10072-021-05314-y.Epub ahead of print.PMID:33988800.
Species: Human
EK0522 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Cavdar Z, Ozbal S, Celik A, Ergur Bu, Guneli E, Ural C, Camsari T, Guner Ga. Biotech Histochem. 2014 May;89(4):304-14. Doi: 10.3109/10520295.2013.847498. Epub 2013 Oct 28. The Effects Of Alpha-Lipoic Acid On Mmp-2 And Mmp-9 Activities In A Rat Ren...
Xu Xl, Ling Dy, Zhu Qy, Fan Wj, Zhang W. Eur J Pharmacol. 2013 Jan 5;698(1-3):370-8. Doi: 10.1016/J.Ejphar.2012.11.019. Epub 2012 Nov 21. The Effect Of 2,3,4',5-Tetrahydroxystilbene-2-0-??-D Glucoside On Neointima Formation In A Rat Artery Balloon...
Yu X, Zhu J, Mi M, Chen W, Pan Q, Wei M. Med Oncol. 2012 Mar;29(1):349-57. Doi: 10.1007/S12032-010-9770-2. Epub 2010 Dec 4. Anti-Angiogenic Genistein Inhibits Vegf-Induced Endothelial Cell Activation By Decreasing Ptk Activity And Mapk Activation.
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Customer Reviews
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Customer Q&As
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9 Customer Q&As for Human TIMP-2 ELISA Kit PicoKine®
Question
Q: What is the optimal O.D. value for TIMP-2 ELISA kit? I used your TIMP-2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain TIMP-2 even though the O.D. values are not very high?
Z. Robinson
Verified customer
Asked: 2020-05-08
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this TIMP-2 ELISA kit to have sensitivity of 2pg/ml, that means the minimum amount of TIMP-2 that can be declared/interpreted as positive by the above standard is 2pg/ml.
Boster Scientific Support
Answered: 2020-05-08
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
W. Hill
Verified customer
Asked: 2019-02-28
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-02-28
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification TIMP-2 using the EK0522 Human TIMP-2 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-11-10
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2018-11-10
Question
Q: how do I analyze ELISA data? I measured TIMP-2 level in serum.
D. Mehta
Verified customer
Asked: 2018-10-23
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-10-23
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2018-10-02
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-10-02
Question
Q: can I use citrate plasma as samples in Human TIMP-2 Picokine® ELISA Kit (Catalog # EK0522)?
R. Green
Verified customer
Asked: 2018-01-04
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of TIMP-2 causing disruption of its protein structure. TIMP-2 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the TIMP-2 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-01-04
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
J. Babu
Verified customer
Asked: 2018-01-03
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-01-03
Question
Q: how to thaw whole blood sample for TIMP-2 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2016-11-26
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test TIMP-2 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2016-11-26
Question
Q: if the enzyme conjugated TIMP-2 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TIMP-2 antigen?
A. Adams
Verified customer
Asked: 2016-07-04
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2016-07-04
